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AB133583

Anti-CENPE antibody [EPR4542(2)]

5

(1 Review)

|

(13 Publications)

Rabbit Recombinant Monoclonal CENPE antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications.

View Alternative Names

Centromere-associated protein E, Centromere protein E, Kinesin-7, Kinesin-related protein CENPE, CENP-E, CENPE

3 Images
Western blot - Anti-CENPE antibody [EPR4542(2)] (AB133583)
  • WB

Unknown

Western blot - Anti-CENPE antibody [EPR4542(2)] (AB133583)

All lanes:

Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583) at 1/1000 dilution

Lane 1:

HeLa treated with aphidicolin and Nocodazole cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 316 kDa

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Western blot - Anti-CENPE antibody [EPR4542(2)] (AB133583)
  • WB

Lab

Western blot - Anti-CENPE antibody [EPR4542(2)] (AB133583)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CENPE knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab133583 observed at 310 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133583 was shown to specifically react with CENPE when CENPE knockout samples were used. Wild-type and CENPE knockout samples were subjected to SDS-PAGE. ab133583 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CENPE antibody [EPR4542(2)] (ab133583)

Predicted band size: 316 kDa

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OI-RD Scanning - Anti-CENPE antibody [EPR4542(2)] (AB133583)
  • OI-RD Scanning

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OI-RD Scanning - Anti-CENPE antibody [EPR4542(2)] (AB133583)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-CENPE antibody [EPR4542(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4542(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPE also known as centromere-associated protein E or kinesin-like protein CENP-E plays a critical role in the alignment of chromosomes during mitosis. This protein with a molecular mass of approximately 312 kDa acts as a motor protein involved in chromosome movement. CENPE is mainly expressed in dividing cells and plays an important role during the metaphase stage of the cell cycle. The protein is localized at the kinetochores the protein complexes on chromatids where the spindle fibers attach during cell division.
Biological function summary

CENPE is a vital component for chromosome congression which ensures proper chromosome alignment and segregation. It is part of several essential complexes necessary for mitotic checkpoint signaling and the correction of chromosome-microtubule attachments. Without CENPE's function cells face increased risks of aneuploidy. The protein interacts with microtubules to drive the movement toward the center of the cell and is important in ensuring accurate cell division.

Pathways

CENPE is involved in the mitotic checkpoint and spindle assembly pathways. These pathways ensure the correct distribution of chromosomes during cell division and prevent genetic instability. CENPE interacts with other proteins such as BUBR1 and Mad2 which are integral parts of the mitotic checkpoint complex. Proper functioning of CENPE ensures the activation of these checkpoints preventing premature separation of chromatids and maintaining genomic integrity.

CENPE has links to various conditions characterized by chromosomal instability such as cancer. Aberrant expression or dysfunction of CENPE can lead to improper chromosome segregation contributing to aneuploidy a hallmark of many cancers. Moreover its function connects with other proteins like Aurora B kinase which also plays a role in chromosomal alignment and stability. Disruption in the functions of these proteins can promote tumor progression and malignancy.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules (PubMed : 23891108, PubMed : 25395579, PubMed : 7889940). The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated (PubMed : 25908662). Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends (PubMed : 23955301). Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (PubMed : 25743205). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed : 17535814) The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (By similarity).
See full target information CENPE
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cancer biomarkers : section A of Disease markers 41:165-178 PubMed39331093

2024

KIF18A promotes cervical squamous cell carcinoma progression by activating the PI3K/AKT pathway through upregulation of CENPE.

Applications

Unspecified application

Species

Unspecified reactive species

Fengyi Sun,Tiantian Zhao

Nature communications 14:5317 PubMed37658044

2023

CENP-E activation by Aurora A and B controls kinetochore fibrous corona disassembly.

Applications

Unspecified application

Species

Unspecified reactive species

Susana Eibes,Girish Rajendraprasad,Claudia Guasch-Boldu,Mirela Kubat,Yulia Steblyanko,Marin Barisic

EMBO reports 24:e56100 PubMed37291955

2023

The GCN2/eIF2αK stress kinase regulates PP1 to ensure mitotic fidelity.

Applications

Unspecified application

Species

Unspecified reactive species

Vilte Stonyte,Maria Mastrangelopoulou,Romy Timmer,Lilian Lindbergsengen,Marina Vietri,Coen Campsteijn,Beáta Grallert

Nature communications 13:7307 PubMed36435852

2022

Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Jurica Matković,Subhadip Ghosh,Mateja Ćosić,Susana Eibes,Marin Barišić,Nenad Pavin,Iva M Tolić

Current biology : CB 32:4240-4254.e5 PubMed36057259

2022

Micronuclei from misaligned chromosomes that satisfy the spindle assembly checkpoint in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Margarida Gomes,Bernardo Orr,Marco Novais-Cruz,Filipe De Sousa,Joana Macário-Monteiro,Carolina Lemos,Cristina Ferrás,Helder Maiato

Cell reports 39:110610 PubMed35385739

2022

Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Ana C Almeida,Joana Soares-de-Oliveira,Danica Drpic,Liam P Cheeseman,Joana Damas,Harris A Lewin,Denis M Larkin,Paulo Aguiar,António J Pereira,Helder Maiato

The EMBO journal 39:e105432 PubMed33073400

2020

Microtubule poleward flux in human cells is driven by the coordinated action of four kinesins.

Applications

Unspecified application

Species

Unspecified reactive species

Yulia Steblyanko,Girish Rajendraprasad,Mariana Osswald,Susana Eibes,Ariana Jacome,Stephan Geley,António J Pereira,Helder Maiato,Marin Barisic

European journal of cell biology 99:151107 PubMed32800279

2020

Kinesin-7 CENP-E regulates cell division, gastrulation and organogenesis in development.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Wei Yu,Zhen-Yu She,Ya-Lan Wei,Ning Zhong

Translational cancer research 9:717-725 PubMed35117417

2020

CENPE promotes glioblastomas proliferation by directly binding to WEE1.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanling Ma,Jianchao Wang,Jie Zhou,Kaisen Liao,Min Yang,Fangqiong Li,Meng Zhang

International journal of oncology 55:257-266 PubMed31115500

2019

CENPE promotes lung adenocarcinoma proliferation and is directly regulated by FOXM1.

Applications

Unspecified application

Species

Unspecified reactive species

Lina Shan,Minjie Zhao,Ya Lu,Hongjuan Ning,Shuman Yang,Yonggui Song,Wenshu Chai,Xianbao Shi
View all publications
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