AB124733

Anti-CENPE antibody [EPR4543(2)]

Name: Anti-CENPE antibody [EPR4543(2)] (ab124733) | Abcam
Brand: Abcam Limited
SKU: AB124733
Price: 380 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal CENPE antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Centromere-associated protein E, Centromere protein E, Kinesin-7, Kinesin-related protein CENPE, CENP-E, CENPE

2 Images

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Western blot - Anti-CENPE antibody [EPR4543(2)] (AB124733)

All lanes:

Western blot - Anti-CENPE antibody [EPR4543(2)] (ab124733) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 316 kDa

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Lab

Western blot - Anti-CENPE antibody [EPR4543(2)] (AB124733)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CENPE knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : HepG2 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab124733 observed at 310 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124733 was shown to specifically react with CENPE when CENPE knockout samples were used. Wild-type and CENPE knockout samples were subjected to SDS-PAGE. ab124733 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CENPE antibody [EPR4543(2)] (ab124733)

Predicted band size: 316 kDa

false

Carrier free

Anti-CENPE antibody [EPR4543(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4543(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPE also known as centromere-associated protein E or kinesin-like protein CENP-E plays a critical role in the alignment of chromosomes during mitosis. This protein with a molecular mass of approximately 312 kDa acts as a motor protein involved in chromosome movement. CENPE is mainly expressed in dividing cells and plays an important role during the metaphase stage of the cell cycle. The protein is localized at the kinetochores the protein complexes on chromatids where the spindle fibers attach during cell division.

Biological function summary

CENPE is a vital component for chromosome congression which ensures proper chromosome alignment and segregation. It is part of several essential complexes necessary for mitotic checkpoint signaling and the correction of chromosome-microtubule attachments. Without CENPE's function cells face increased risks of aneuploidy. The protein interacts with microtubules to drive the movement toward the center of the cell and is important in ensuring accurate cell division.

Pathways

CENPE is involved in the mitotic checkpoint and spindle assembly pathways. These pathways ensure the correct distribution of chromosomes during cell division and prevent genetic instability. CENPE interacts with other proteins such as BUBR1 and Mad2 which are integral parts of the mitotic checkpoint complex. Proper functioning of CENPE ensures the activation of these checkpoints preventing premature separation of chromatids and maintaining genomic integrity.

CENPE has links to various conditions characterized by chromosomal instability such as cancer. Aberrant expression or dysfunction of CENPE can lead to improper chromosome segregation contributing to aneuploidy a hallmark of many cancers. Moreover its function connects with other proteins like Aurora B kinase which also plays a role in chromosomal alignment and stability. Disruption in the functions of these proteins can promote tumor progression and malignancy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules (PubMed : 23891108, PubMed : 25395579, PubMed : 7889940). The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated (PubMed : 25908662). Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends (PubMed : 23955301). Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized (PubMed : 25743205). Plays an important role in the formation of stable attachments between kinetochores and spindle microtubules (PubMed : 17535814) The stabilization of kinetochore-microtubule attachment also requires CENPE-dependent localization of other proteins to the kinetochore including BUB1B, MAD1 and MAD2. Plays a role in spindle assembly checkpoint activation (SAC) via its interaction with BUB1B resulting in the activation of its kinase activity, which is important for activating SAC. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss (By similarity).

See full target information CENPE

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Respiratory research 25:426 PubMed39633451

2024

Loss of PTPN21 disrupted mitochondrial metabolic homeostasis and aggravated experimental pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Lian,Kai Xu,Airu Chang,Yaxuan Wang,Shuaichen Ma,Lianhui Cheng,Wenyu Zhao,Cong Xia,Lan Wang,Guoying Yu

Canadian respiratory journal 2022:9663354 PubMed36247080

2022

Inhibition of MAD2L1 Mediates Pulmonary Fibrosis through Impairment of Mitochondrial Function and Induction of Cell Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Wang,Ruyan Wan,Xinyu Chen,Xiaoshu Guo,Zhongzheng Li,Weiming Zhao,Peishuo Yan,Guoying Yu

Frontiers in neuroscience 16:814430 PubMed35368248

2022

Neuroprotective Effects of a Cholecystokinin Analogue in the 1-Methyl-4-Phenyl-1,2,3,6-Tetrahydropyridine Parkinson's Disease Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Zijuan Zhang,Hai Li,Yunfang Su,Jinlian Ma,Ye Yuan,Ziyang Yu,Ming Shi,Simai Shao,Zhenqiang Zhang,Christian Hölscher

Bioengineered 12:5950-5970 PubMed34482803

2021

Centromere protein E as a novel biomarker and potential therapeutic target for retinoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Shi,Xinyue Zhu,Jiali Wu,Yuhong Chen,Jingfa Zhang,Xiaodong Sun

Nature communications 7:13887 PubMed27976684

2016

Profiling DNA damage response following mitotic perturbations.

Applications

Unspecified application

Species

Unspecified reactive species

Ronni S Pedersen,Gopal Karemore,Thorkell Gudjonsson,Maj-Britt Rask,Beate Neumann,Jean-Karim Hériché,Rainer Pepperkok,Jan Ellenberg,Daniel W Gerlich,Jiri Lukas,Claudia Lukas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CENPE antibody [EPR4543(2)]

Western blot - Anti-CENPE antibody [EPR4543(2)] (AB124733)

Western blot - Anti-CENPE antibody [EPR4543(2)] (AB124733)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise