Rabbit Polyclonal CENPF antibody. Suitable for WB, ICC, IP, Flow Cyt, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 108 publications. Immunogen corresponding to Fusion protein corresponding to Centromere protein F, fused to Centromere protein F.
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- iScience 26:1068622023macrophages promote hepatocellular carcinoma tumor stemness and progression through /β-catenin/ axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Chao Zhao,Chang-Zhou Chen,Yan-Zhang Tian,Huang-Qin Song,Xiao-Xiao Wang,Yan-Jun Li,Jie-Feng He,Hao-Liang ZhaoPubMed 37275516
- Molecular cell 82:1924-1939.e102022H3K4 methylation by SETD1A/BOD1L facilitates RIF1-dependent NHEJ.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRachel Bayley,Valerie Borel,Rhiannon J Moss,Ellie Sweatman,Philip Ruis,Alice Ormrod,Amalia Goula,Rachel M A Mottram,Tyler Stanage,Graeme Hewitt,Marco Saponaro,Grant S Stewart,Simon J Boulton,Martin R HiggsPubMed 35439434
- Bioengineered 12:7780-77932021Long non-coding RNA LINC01207 promotes cell proliferation and migration but suppresses apoptosis and autophagy in oral squamous cell carcinoma by the microRNA-1301-3p/lactate dehydrogenase isoform A axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolin Lu,Liling Chen,Yang Li,Rong Huang,Xiangfeng Meng,Fangfang SunPubMed 34463208
- Signal transduction and targeted therapy 6:3222021VAV2 is required for DNA repair and implicated in cancer radiotherapy resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiling Liu,Chuanwang Miao,Shaosen Zhang,Yachen Liu,Xiangjie Niu,Yiyi Xi,Wenjia Guo,Jiahui Chu,Ai Lin,Hongjin Liu,Xinyu Yang,Xinjie Chen,Ce Zhong,Yuling Ma,Yuqian Wang,Shihao Zhu,Shuning Liu,Wen Tan,Dongxin Lin,Chen WuPubMed 34462423
- Molecular medicine reports 24:2021Role of the CXCR3‑mediated TLRs/MyD88 signaling pathway in promoting the development of hepatitis B into cirrhosis and liver cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Yuan,Bin Chen,Yina Meng,Jialin Lu,Xiaojun Shi,Airong Hu,Yaoren Hu,Donghui WangPubMed 34435646
- Yonsei medical journal 62:843-8492021Temozolomide Drives Ferroptosis via a DMT1-Dependent Pathway in Glioblastoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingxin Song,Shanxin Peng,Zhiqing Sun,Xueyuan Heng,Xiaosong ZhuPubMed 34427071
- Cancer management and research 13:6599-66092021CircRNA DUSP16 Knockdown Suppresses Colorectal Cancer Progression by Regulating the miR-432-5p/E2F6 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangyao Wang,Haojun YangPubMed 34456589
- Journal of cellular and molecular medicine 25:8973-89842021In vivo visualization and characterization of inflamed intestinal wall: the exploration of targeted microbubbles in assessing NF-κB expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenyang Zhao,Li Ma,Yanwen Luo,Wenbo Li,Mengsu Xiao,Qingli Zhu,Yuxin JiangPubMed 34409723
- BioMed research international 2021:34563212021Melatonin Inhibits Annulus Fibrosus Cell Senescence through Regulating the ROS/NF-B Pathway in an Inflammatory Environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Li,Jianghua Li,Chengzhang Cao,Jianhua Sun,Sibo Wang,Zhi Ruan,Lei Chen,Ke LiPubMed 34458366
- Biology 10:2021Connexin 43 and Sonic Hedgehog Pathway Interplay in Glioblastoma Cell Proliferation and Migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFilippo Torrisi,Cristiana Alberghina,Debora Lo Furno,Agata Zappalà,Samuel Valable,Giovanni Li Volti,Daniele Tibullo,Nunzio Vicario,Rosalba ParentiPubMed 34439999
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Fusion protein corresponding to Centromere protein F, fused to Centromere protein F. The exact immunogen used to generate this antibody is proprietary information. Database link P49454
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Reactivity data
Select an application| WB | ICC | IP | Flow Cyt | IHC-P | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Expected | Expected | Expected | Tested | Tested |
| Mouse | Predicted | Expected | Expected | Expected | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Block membranes for 1 hour with 5% nonfat dry milk/TBS-T. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 - 1/750 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.
Alternative names
Centromere protein F, CENP-F, AH antigen, Kinetochore protein CENPF, Mitosin, CENPF
Recommended products
Rabbit Polyclonal CENPF antibody. Suitable for WB, ICC, IP, Flow Cyt, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 108 publications. Immunogen corresponding to Fusion protein corresponding to Centromere protein F, fused to Centromere protein F.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Fusion protein corresponding to Centromere protein F, fused to Centromere protein F. The exact immunogen used to generate this antibody is proprietary information. Database link P49454
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CENPF also known as centromere protein F or mitosin is a significant kinetochore-associated protein. It has a molecular mass of approximately 367 kDa. Expressed chiefly in the nucleus CENPF displays elevated levels during cell division particularly mitosis. The protein accumulates at the nuclear matrix during the G2 phase of the cell cycle and moves to the kinetochores in mitosis highlighting its role in cell cycle regulation.
- Biological function summary
It plays a role in chromosome segregation and spindle attachment during cell division. CENPF associates with various cellular complexes including the nuclear matrix and kinetochores. Its function is critical to maintaining stability and proper chromosome movement during mitosis. This involvement ensures accurate chromosomal alignment and segregation preventing genomic instability in daughter cells.
- Pathways
CENPF plays an essential role in the regulation of the mitotic cell cycle and chromosomal dynamics. The protein interfaces with several elements in pathways like the spindle assembly checkpoint. It interacts notably with proteins such as CENP-E and other kinetochore constituents. These interactions safeguard the precise attachment of microtubules to the kinetochores facilitating proper cell cycle progression and division.
- Associated diseases and disorders
Alterations in CENPF expression and function have been linked to cancer and congenital heart defects. Dysregulation of CENPF can lead to chromosomal instability a known hallmark of many cancers. Additionally its interactions with proteins like CENP-E and Bub1 further relate to its involvement in the proper execution of cell cycle checkpoints. Such associations make CENPF a potential target for therapeutic interventions in related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
This image is courtesy of Kirk McManus, University of British ColumbiaImmunocytochemistry/ Immunofluorescence - Anti-CENPF antibody (ab5)
HeLa cells were labelled with anti-ACA and anti-CENPF (ab5). ab5 was used at a working dilution of 1/400. This image demonstrates the dramatic increase in fluorescence that occurs late in G2 cells (indicated by arrows). In the final panel DAPI is pseudo-coloured blue, while ACA and CENPF are coloured green and red respectively. 40x magnification.
Immunocytochemistry/ Immunofluorescence - Anti-CENPF antibody (ab5)
ICC/IF image of ab5 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab5, 1μg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPF antibody (ab5)
ab5 staining Human normal colon tissue. Staining is localised to nuclear compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
This image is courtesy of Kirk McManus, University of British ColumbiaImmunocytochemistry/ Immunofluorescence - Anti-CENPF antibody (ab5)
Interpahse and Prophase HeLa cells were labelled with anti-ACA and anti-CENPF (ab5). ab5 was used at a working dilution of 1/400. This image emphasizes the redistribution of CENPF from the nuclear matrix during late G2 following entry into the initial stages of mitosis (see the accompanying image). Distinct punctate CENPF patterns proximally located in relation to the centromeres can be seen. In the final panel DAPI is pseudo-coloured blue, while ACA and CENPF are green and red respectively. 100x magnification.
Western blot - Anti-CENPF antibody (ab5)
All lanes: Western blot - Anti-CENPF antibody (ab5) at 1/1500 dilution
Lane 1: Mitotic HeLa Lysate at 25 µg
Lane 2: Asynchronous HeLa Lysate at 25 µg
Lane 3: 50gamma Mitotic HeLa Lysate at 25 µg
Lane 4: 50gamma Asynchronous HeLa Lysate at 25 µg
Predicted band size: 367 kDa
Observed band size: 310 kDa
This image is courtesy of a customer review submitted by Dr. Stuart Rulten.Immunocytochemistry/ Immunofluorescence - Anti-CENPF antibody (ab5)
Paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for CENPF (red) using ab5 at 1/500 dilution in ICC/IF. ). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) at 1/500 dilution was used as the secondary antibody.
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