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AB224159

Anti-CENPH antibody

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(1 Publication)

Rabbit Polyclonal CENPH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CENPH aa 100 to C-terminus.

View Alternative Names

ICEN35, CENPH, Centromere protein H, CENP-H, Interphase centromere complex protein 35

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPH antibody (AB224159)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPH antibody (AB224159)

Paraffin-embedded human small intestine tissue stained for CENPH using ab224159 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CENPH antibody (AB224159)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CENPH antibody (AB224159)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CENPH (green) using ab224159 at 4 μg/ml in ICC/IF.

Western blot - Anti-CENPH antibody (AB224159)
  • WB

Supplier Data

Western blot - Anti-CENPH antibody (AB224159)

All lanes:

Western blot - Anti-CENPH antibody (ab224159)

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 28 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CENPH aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H3R5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPH also known as Centromere Protein H is part of the inner kinetochore complex which plays an important role in chromosome segregation during cell division. The protein has a molecular mass of approximately 29 kDa. CENPH is expressed primarily in dividing cells including those in the bone marrow and lymphoid tissues. High expression levels are observed in cancerous tissues suggesting a link to cell proliferation.
Biological function summary

CENPH plays an essential role in the assembly and stabilization of the kinetochore complex. It forms part of the CENP-A nucleosome-associated complex (NAC) critical for kinetochore function. CENPH ensures accurate attachment of spindle microtubules to the kinetochore which is necessary for proper chromosome alignment and segregation. The presence of CENPH in the kinetochore safeguards against chromosomal instability.

Pathways

CENPH contributes significantly to the cell cycle and mitotic spindle checkpoint pathways. It interacts intricately with other centromere proteins like CENP-C and CENP-A coordinating the dynamics of kinetochore and centromere function during cell division. Disruptions in any of these interactions can lead to errors in chromosome segregation which highlights CENPH's importance within these pathways.

CENPH has been associated with cancer and genetic instability disorders. Aberrant expression or mutation in CENPH can lead to missegregation of chromosomes a hallmark of cancerous cells. It is often studied in conjunction with proteins such as CENP-A given their collective contribution to the integrity of the centromere. Understanding CENPH's role in these conditions provides insight into potential therapeutic targets for cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
See full target information CENPH
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of oncology 57:1001-1012 PubMed32945386

2020

lncRNA NEAT1 regulates the proliferation and migration of hepatocellular carcinoma cells by acting as a miR‑320a molecular sponge and targeting L antigen family member 3.

Applications

Unspecified application

Species

Unspecified reactive species

Yixi Zhang,Changjun Huang,Zebin Zhu,Yufei Hou,Shanzhou Huang,Chengjun Sun,Yunhua Tang,Zhiheng Zhang,Linhe Wang,Huadi Chen,Weiqiang Ju,Xin Qiao,Maogen Chen
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Product promise

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For full details, please see our Terms & Conditions

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