Rabbit Polyclonal CENPH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CENPH aa 100 to C-terminus.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CENPH aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H3R5
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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Target data
Function
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
Alternative names
ICEN35, CENPH, Centromere protein H, CENP-H, Interphase centromere complex protein 35
Recommended products
Rabbit Polyclonal CENPH antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CENPH aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CENPH aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H3R5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CENPH also known as Centromere Protein H is part of the inner kinetochore complex which plays an important role in chromosome segregation during cell division. The protein has a molecular mass of approximately 29 kDa. CENPH is expressed primarily in dividing cells including those in the bone marrow and lymphoid tissues. High expression levels are observed in cancerous tissues suggesting a link to cell proliferation.
- Biological function summary
CENPH plays an essential role in the assembly and stabilization of the kinetochore complex. It forms part of the CENP-A nucleosome-associated complex (NAC) critical for kinetochore function. CENPH ensures accurate attachment of spindle microtubules to the kinetochore which is necessary for proper chromosome alignment and segregation. The presence of CENPH in the kinetochore safeguards against chromosomal instability.
- Pathways
CENPH contributes significantly to the cell cycle and mitotic spindle checkpoint pathways. It interacts intricately with other centromere proteins like CENP-C and CENP-A coordinating the dynamics of kinetochore and centromere function during cell division. Disruptions in any of these interactions can lead to errors in chromosome segregation which highlights CENPH's importance within these pathways.
- Associated diseases and disorders
CENPH has been associated with cancer and genetic instability disorders. Aberrant expression or mutation in CENPH can lead to missegregation of chromosomes a hallmark of cancerous cells. It is often studied in conjunction with proteins such as CENP-A given their collective contribution to the integrity of the centromere. Understanding CENPH's role in these conditions provides insight into potential therapeutic targets for cancer treatment.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-CENPH antibody (ab224159)
All lanes: Western blot - Anti-CENPH antibody (ab224159)
All lanes: RT4 (human urinary bladder cancer cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 28 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CENPH antibody (ab224159)
Paraffin-embedded human small intestine tissue stained for CENPH using ab224159 at 1/200 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-CENPH antibody (ab224159)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CENPH (green) using ab224159 at 4 μg/ml in ICC/IF.
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