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AB88593

Anti-CENPH antibody

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(2 Publications)

Mouse Polyclonal CENPH antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CENPH.

View Alternative Names

ICEN35, CENPH, Centromere protein H, CENP-H, Interphase centromere complex protein 35

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-CENPH antibody (AB88593)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CENPH antibody (AB88593)

ab88593 at 10 μg/ml detecting CENPH in HeLa cells by immunofluorescence.

Western blot - Anti-CENPH antibody (AB88593)
  • WB

Unknown

Western blot - Anti-CENPH antibody (AB88593)

All lanes:

Western blot - Anti-CENPH antibody (ab88593) at 5 µg/mL

All lanes:

Raw 264.7 cell lysate at 25 µg

Predicted band size: 28 kDa

Observed band size: 28 kDa

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Western blot - Anti-CENPH antibody (AB88593)
  • WB

Unknown

Western blot - Anti-CENPH antibody (AB88593)

All lanes:

Western blot - Anti-CENPH antibody (ab88593) at 5 µg/mL

All lanes:

NIH/3T3 cell lysate at 25 µg

Predicted band size: 28 kDa

Observed band size: 28 kDa,~19 kDa,~60 kDa,~70 kDa

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Western blot - Anti-CENPH antibody (AB88593)
  • WB

Unknown

Western blot - Anti-CENPH antibody (AB88593)

All lanes:

Western blot - Anti-CENPH antibody (ab88593) at 5 µg/mL

Lane 1:

cell lysate from CENPH transfected 293T cells at 25 µg

Lane 2:

cell lysate from non transfected 293T cells at 25 µg

Predicted band size: 28 kDa

Observed band size: 28 kDa

true

Western blot - Anti-CENPH antibody (AB88593)
  • WB

Unknown

Western blot - Anti-CENPH antibody (AB88593)

All lanes:

Western blot - Anti-CENPH antibody (ab88593) at 5 µg/mL

All lanes:

HeLa cell lysate at 25 µg

Predicted band size: 28 kDa

Observed band size: 28 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CENPH.

Q9H3R5

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Alternative Products

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AB118796

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPH also known as Centromere Protein H is part of the inner kinetochore complex which plays an important role in chromosome segregation during cell division. The protein has a molecular mass of approximately 29 kDa. CENPH is expressed primarily in dividing cells including those in the bone marrow and lymphoid tissues. High expression levels are observed in cancerous tissues suggesting a link to cell proliferation.
Biological function summary

CENPH plays an essential role in the assembly and stabilization of the kinetochore complex. It forms part of the CENP-A nucleosome-associated complex (NAC) critical for kinetochore function. CENPH ensures accurate attachment of spindle microtubules to the kinetochore which is necessary for proper chromosome alignment and segregation. The presence of CENPH in the kinetochore safeguards against chromosomal instability.

Pathways

CENPH contributes significantly to the cell cycle and mitotic spindle checkpoint pathways. It interacts intricately with other centromere proteins like CENP-C and CENP-A coordinating the dynamics of kinetochore and centromere function during cell division. Disruptions in any of these interactions can lead to errors in chromosome segregation which highlights CENPH's importance within these pathways.

CENPH has been associated with cancer and genetic instability disorders. Aberrant expression or mutation in CENPH can lead to missegregation of chromosomes a hallmark of cancerous cells. It is often studied in conjunction with proteins such as CENP-A given their collective contribution to the integrity of the centromere. Understanding CENPH's role in these conditions provides insight into potential therapeutic targets for cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
See full target information CENPH
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 47:468-479 PubMed30407575

2018

Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment.

Applications

Unspecified application

Species

Unspecified reactive species

Liqiao Hu,Hao Huang,Mohan Hei,Yang Yang,Sheng Li,Yunshan Liu,Zhen Dou,Mengying Wu,Jie Li,Guang-Zhong Wang,Xuebiao Yao,Hong Liu,Xiaojing He,Wei Tian

Development (Cambridge, England) 144:305-312 PubMed27993978

2016

CenpH regulates meiotic G2/M transition by modulating the APC/CCdh1-cyclin B1 pathway in oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Zhang,Yang Zhou,Li Li,Zhen-Bo Wang,Wei Shen,Heide Schatten,Qing-Yuan Sun
View all publications
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Product promise

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