AB118796

Anti-CENPI antibody

Name: Anti-CENPI antibody (ab118796) | Abcam
Brand: Abcam Limited
SKU: AB118796
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal CENPI antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human CENPI aa 50-100.

View Alternative Names

FSHPRH1, ICEN19, LRPR1, CENPI, Centromere protein I, CENP-I, FSH primary response protein 1, Follicle-stimulating hormone primary response protein, Interphase centromere complex protein 19, Leucine-rich primary response protein 1

2 Images

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Immunoprecipitation - Anti-CENPI antibody (AB118796)

Detection of CENPI in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118796 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-CENPI antibody (ab118796)

Predicted band size: 87 kDa

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Western blot - Anti-CENPI antibody (AB118796)

All lanes:

Western blot - Anti-CENPI antibody (ab118796) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 87 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CENPI aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

Q92674

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPI also known as centromere protein I is part of the CENP family and plays a mechanical role in centromere assembly and function. It has a molecular mass of approximately 97 kDa. CENPI is expressed in various tissues but it shows higher expression levels in proliferating cells especially during cell division. It localizes to the centromere region of chromosomes and is essential for the proper functioning of the kinetochore the protein structure on chromosomes where spindle fibers attach during cell division.

Biological function summary

The role of CENPI involves its participation in the CENP-A nucleosome-associated complex. This complex is essential for the recruitment of other kinetochore proteins ensuring accurate chromosome segregation during mitosis. The proper functioning of CENPI contributes to maintaining genomic stability as errors in chromosome segregation can lead to aneuploidy which is an abnormal number of chromosomes in cells. Therefore CENPI acts as an important mediator in the accurate distribution of chromosomes during cell division.

Pathways

CENPI plays a significant role in the cell cycle regulation and mitotic checkpoint pathways. It aids in the activation and stability of proteins like CENP-H and CENP-K which cooperate during the early stages of centromere and kinetochore formation. This activity ensures that the assembling of the mitotic spindle is well-regulated and that cells do not prematurely progress through mitosis. Properly functioning CENPI and its pathway partners are critical to preventing cell division errors that could lead to various cellular dysfunctions.

Malfunction or aberrant expression of CENPI has been linked to certain cancers such as colorectal and breast cancer. Disrupted CENPI function can lead to improper chromosome segregation contributing to genetic instability and tumor progression. Additionally studies have pointed to connections between CENPI dysregulation and CENP-E another centromere protein. Together abnormalities in these proteins can exacerbate the risk of oncogenesis and promote aggressive tumor behavior highlighting the importance of the CENP family in controlling cell cycle fidelity and preventing disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Required for the localization of CENPF, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to kinetochores. Involved in the response of gonadal tissues to follicle-stimulating hormone.

See full target information CENPI

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Technology in cancer research & treatment 20:15330338211045510 PubMed34617858

2021

Centromere Protein I (CENP-I) Is Upregulated in Gastric Cancer, Predicts Poor Prognosis, and Promotes Tumor Cell Proliferation and Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Wang,Xin Liu,Hong-Jin Chu,Ning Li,Liu-Ye Huang,Jian Chen

Frontiers in oncology 10:162 PubMed32133296

2020

Validation of Novel Prognostic Biomarkers for Early-Stage Clear-Cell, Endometrioid and Mucinous Ovarian Carcinomas Using Immunohistochemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Engqvist,Toshima Z Parris,Anikó Kovács,Elisabeth Werner Rönnerman,Karin Sundfeldt,Per Karlsson,Khalil Helou

Epigenetics & chromatin 11:68 PubMed30445992

2018

High-resolution mapping of centromeric protein association using APEX-chromatin fibers.

Applications

Unspecified application

Species

Unspecified reactive species

Eftychia Kyriacou,Patrick Heun

Oncotarget 8:62167-62182 PubMed28977935

2017

Overexpression of the E2F target gene promotes chromosome instability and predicts poor prognosis in estrogen receptor-positive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pulari U Thangavelu,Cheng-Yu Lin,Srividya Vaidyanathan,Thu H M Nguyen,Eloise Dray,Pascal H G Duijf

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CENPI antibody

Immunoprecipitation - Anti-CENPI antibody (AB118796)

Western blot - Anti-CENPI antibody (AB118796)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise