AB168778

Anti-CENPI antibody

Name: Anti-CENPI antibody (ab168778) | Abcam
Brand: Abcam Limited
SKU: AB168778

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(3 Publications)

Mouse Polyclonal CENPI antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CENPI.

View Alternative Names

FSHPRH1, ICEN19, LRPR1, CENPI, Centromere protein I, CENP-I, FSH primary response protein 1, Follicle-stimulating hormone primary response protein, Interphase centromere complex protein 19, Leucine-rich primary response protein 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CENPI antibody (AB168778)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CENPI antibody (AB168778)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling CENPI using ab168778 at 10 µg/ml.

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Western blot - Anti-CENPI antibody (AB168778)

All lanes:

Western blot - Anti-CENPI antibody (ab168778) at 1 µg/mL

Lane 1:

CENPI-transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Predicted band size: 87 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CENPI.

Q92674

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPI also known as centromere protein I is part of the CENP family and plays a mechanical role in centromere assembly and function. It has a molecular mass of approximately 97 kDa. CENPI is expressed in various tissues but it shows higher expression levels in proliferating cells especially during cell division. It localizes to the centromere region of chromosomes and is essential for the proper functioning of the kinetochore the protein structure on chromosomes where spindle fibers attach during cell division.

Biological function summary

The role of CENPI involves its participation in the CENP-A nucleosome-associated complex. This complex is essential for the recruitment of other kinetochore proteins ensuring accurate chromosome segregation during mitosis. The proper functioning of CENPI contributes to maintaining genomic stability as errors in chromosome segregation can lead to aneuploidy which is an abnormal number of chromosomes in cells. Therefore CENPI acts as an important mediator in the accurate distribution of chromosomes during cell division.

Pathways

CENPI plays a significant role in the cell cycle regulation and mitotic checkpoint pathways. It aids in the activation and stability of proteins like CENP-H and CENP-K which cooperate during the early stages of centromere and kinetochore formation. This activity ensures that the assembling of the mitotic spindle is well-regulated and that cells do not prematurely progress through mitosis. Properly functioning CENPI and its pathway partners are critical to preventing cell division errors that could lead to various cellular dysfunctions.

Malfunction or aberrant expression of CENPI has been linked to certain cancers such as colorectal and breast cancer. Disrupted CENPI function can lead to improper chromosome segregation contributing to genetic instability and tumor progression. Additionally studies have pointed to connections between CENPI dysregulation and CENP-E another centromere protein. Together abnormalities in these proteins can exacerbate the risk of oncogenesis and promote aggressive tumor behavior highlighting the importance of the CENP family in controlling cell cycle fidelity and preventing disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Required for the localization of CENPF, MAD1L1 and MAD2 (MAD2L1 or MAD2L2) to kinetochores. Involved in the response of gonadal tissues to follicle-stimulating hormone.

See full target information CENPI

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:348 PubMed41074145

2025

Centromere protein I facilitates breast cancer tumorigenesis and disease progression through modulation of Wnt/β-Catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chaoshen Wu,Yibing Zhou,Yuxiao Mu,Yijie Cheng,Wenqian Xu,Mengna Huang,Jingyuan Xu,Ming Li,Jianfeng Gu,Xuli Meng,Da Qian

Frontiers in oncology 15:1546723 PubMed40406242

2025

The mA reader YTHDF3 promotes TNBC progression by regulating CENPI stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Yulu Zhang,Shunji Chen,Qiaosheng Wu

Nucleic acids research 47:468-479 PubMed30407575

2018

Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment.

Applications

Unspecified application

Species

Unspecified reactive species

Liqiao Hu,Hao Huang,Mohan Hei,Yang Yang,Sheng Li,Yunshan Liu,Zhen Dou,Mengying Wu,Jie Li,Guang-Zhong Wang,Xuebiao Yao,Hong Liu,Xiaojing He,Wei Tian

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CENPI antibody

Immunocytochemistry/ Immunofluorescence - Anti-CENPI antibody (AB168778)

Western blot - Anti-CENPI antibody (AB168778)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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