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AB105742

Anti-CENPQ antibody

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(2 Publications)

Rabbit Polyclonal CENPQ antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CENPQ.

View Alternative Names

C6orf139, CENPQ, Centromere protein Q, CENP-Q

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CENPQ antibody (AB105742)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CENPQ antibody (AB105742)

Immunofluorescence microscopy using ab105742 shows detection of endogenous CENPQ in HeLa whole cell lysate. Primary antibody was used at 1 : 100 followed by secondary antibody diluted 1 : 150. Red punctate anti-CENPQ signal colocalizes in overlay images with green punctate anti-CREST signals at the kinetochores (attached points of sister chromatids). Visible are colocalized CENPQ and CREST signal at various stages of the cell cycle as indicated from interphase to the end of mitosis. Nuclei are counter stained with bisbenzimide.

Western blot - Anti-CENPQ antibody (AB105742)
  • WB

Unknown

Western blot - Anti-CENPQ antibody (AB105742)

All lanes:

Western blot - Anti-CENPQ antibody (ab105742) at 0.5 µg/mL

Lane 1:

Lysates from HeLa cells transfected with control sh-virus

Lane 2:

Lysates from HeLa cells transfected with CENPQ sh-virus

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CENPQ.

Q7L2Z9

Specificity

This antibody is specific for human CENP-Q protein.  A BLAST analysis of the full length sequence was used to suggest partial cross-reactivity with CENP-Q based on the following percentage homologies: macaque (91%), horse (76%), bovine (74%), dog (73%), swine (71%), mouse (65%) and rat (60%). Cross-reactivity with CENP-Q from other sources has not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CENPQ also known as Centromere Protein Q is a component of the inner kinetochore plate. This protein has an approximate molecular mass of 25 kDa. CENPQ is expressed in various tissues. It localizes at the centromere during cell division. The presence of CENPQ ensures the proper attachment of microtubules to the kinetochore from spindle fibers critical for chromosome alignment and segregation.
Biological function summary

Centromere Protein Q plays a role in the maintenance of centromere structure and function. It forms part of the CENP-H/I/K complex which is involved in assembling the kinetochore. This complex stabilizes the kinetochore-microtubule interactions facilitating the accurate transmission of chromosomes during mitosis. The precise coordination of these activities helps prevent chromosomal instability which could lead to genetic disorders.

Pathways

CENPQ participates in the mitotic spindle checkpoint pathway. This pathway ensures that cells do not progress to anaphase until all chromosomes are properly aligned at the metaphase plate preventing aneuploidy. In addition to this CENPQ interacts with other proteins such as CENP-C and CENP-E. These interactions are essential to kinetochore function and the overall fidelity of cell division.

CENPQ plays a role in cancer and chromosomal instability syndromes. Malfunction or mutations in CENPQ can compromise its ability to maintain proper chromosome segregation which is associated with various forms of cancer. CENPQ's interactions with other centromere proteins like CENP-E are critical for maintaining genomic stability their disruption can lead to tumorigenesis or progression of chromosomal aberrations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex (PubMed : 16622420). Plays an important role in chromosome congression and in the recruitment of CENP-O complex (which comprises CENPO, CENPP, CENPQ and CENPU), CENPE and PLK1 to the kinetochores (PubMed : 25395579).
See full target information CENPQ
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 17:1357-1367 PubMed36046633

2022

LncRNA LINC01857 reduces metastasis and angiogenesis in breast cancer cells via regulating miR-2052/CENPQ axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Qian,Linlin Yang,Yi Ni,Fei Yin,Lili Qin,Yang Yang

Animal bioscience 34:993-1005 PubMed32898953

2020

Zearalenone exposure affects the Wnt/β-catenin signaling pathway and related genes of porcine endometrial epithelial cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Song,Weiren Yang,Libo Huang,Zaibin Yang,Shuzhen Jiang
View all publications
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