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AB220280

Anti-CENPT antibody

5

(1 Review)

|

(6 Publications)

Rabbit Polyclonal CENPT antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CENPT aa 50-200.

View Alternative Names

C16orf56, ICEN22, CENPT, Centromere protein T, CENP-T, Interphase centromere complex protein 22

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CENPT antibody (AB220280)
  • ICC/IF

AbReview60987****

Immunocytochemistry/ Immunofluorescence - Anti-CENPT antibody (AB220280)

ab14404 staining CENPT in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldahyde, permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 21°C. ab150081 (1/200) used as the secondary antibody.

This image is courtesy of an abreview by Kirk Mcmanus

Immunocytochemistry/ Immunofluorescence - Anti-CENPT antibody (AB220280)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CENPT antibody (AB220280)

Immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) whole cells labeling CENPT (green) in the nuclear bodies with ab220280 at 2 μg/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CENPT aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q96BT3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Cynomolgus monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Centromere protein T commonly known as CENPT is an important component of the kinetochore complex. This protein has a molecular mass of approximately 94 kDa and plays an essential role in chromosome segregation during cell division. CENPT primarily localizes to the centromere region of chromosomes and is ubiquitously expressed in dividing cells particularly in human tissues with high cellular turnover. Often referred to as CENP-T it several times forms part of the inner kinetochore binding and directing microtubules for accurate chromosomal movement.
Biological function summary

Centromere protein T engages in stabilizing kinetochores ensuring proper chromosome alignment and segregation. As a part of the constitutive centromere-associated network (CCAN) complex CENPT cooperates closely with other proteins such as CENP-C and CENP-A to maintain kinetochore structure and function. This protein actively participates in facilitating the attachment of spindle microtubules to the centromere orchestrating the dynamic interactions necessary for efficient cell division.

Pathways

The role of CENPT integrates into the mechanistic regulation of the mitotic cell cycle. CENPT is pivotal in the pathway driven by the anaphase-promoting complex/cyclosome (APC/C) which regulates the separation of sister chromatids. Moreover CENPT works in conjunction with the spindle assembly checkpoint ensuring that chromosomes are properly aligned before anaphase onset. This coordination involves interactions with proteins like BUB1 and MAD2 safeguarding the fidelity of chromosome transmission during mitosis.

Centromere protein T has been implicated in chromosomal instability which is a hallmark of certain cancers. Abnormal expression or function of CENPT can lead to aneuploidy contributing to the progression of conditions such as colorectal cancer. Additionally CENPT-related defects have connections to Aurora B kinase a mitotic regulator whose dysfunction associates with cancerous phenotypes. Understanding the role of CENPT in these disorders opens avenues for therapeutic exploration targeting the mitotic machinery to combat malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.
See full target information CENPT
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115259 PubMed39893637

2025

The homologous recombination factors BRCA2 and PALB2 interplay with mismatch repair pathways to maintain centromere stability and cell viability.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Graham,Lucia Rampazzo,Chin Wei Brian Leung,Jacob Wall,Emőke Zsanett Gerőcz,Mikhail Liskovykh,Nikolay Goncharov,Xanita Saayman,Ramazan Gundogdu,Masato T Kanemaki,Hiroshi Masumoto,Vladimir Larionov,Natalay Kouprina,Fumiko Esashi

Cancer science 116:420-431 PubMed39604214

2024

Fibrous corona is reduced in cancer cell lines that attenuate microtubule nucleation from kinetochores.

Applications

Unspecified application

Species

Unspecified reactive species

Yudai Ishikawa,Hirotaka Fukue,Runa Iwakami,Masanori Ikeda,Kenji Iemura,Kozo Tanaka

The EMBO journal 42:e111587 PubMed37063065

2023

Targeted assembly of ectopic kinetochores to induce chromosome-specific segmental aneuploidies.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Tovini,Sarah C Johnson,Molly A Guscott,Alexander M Andersen,Diana Carolina Johanna Spierings,René Wardenaar,Floris Foijer,Sarah E McClelland

Molecular biology of the cell 34:br6 PubMed36989032

2023

p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastiaan J W van den Berg,Samuel East,Sreyoshi Mitra,Lars E T Jansen

Disease markers 2022:2893336 PubMed35371344

2022

Evaluation of Immune Infiltration Based on Image Plus Helps Predict the Prognosis of Stage III Gastric Cancer Patients with Significantly Different Outcomes in Northeastern China.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Fang,Zhidong Wang,Xin Yin,Hao Wang,Lei Zhang,Xuan Lin,Xinghai Zhang,Yimin Wang,Yingwei Xue

Open biology 10:200227 PubMed33081635

2020

Reprogramming of human cells to pluripotency induces CENP-A chromatin depletion.

Applications

Unspecified application

Species

Unspecified reactive species

Inês Milagre,Carolina Pereira,Raquel A Oliveira,Lars E T Jansen
View all publications
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