AB86595

Anti-CENPT antibody

Name: Anti-CENPT antibody (ab86595) | Abcam
Brand: Abcam Limited
SKU: AB86595
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal CENPT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CENPT aa 450-550.

View Alternative Names

C16orf56, ICEN22, CENPT, Centromere protein T, CENP-T, Interphase centromere complex protein 22

2 Images

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Western blot - Anti-CENPT antibody (AB86595)

All lanes:

Western blot - Anti-CENPT antibody (ab86595) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 60 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-CENPT antibody (AB86595)

1mg whole cell lysate from Hela cells was immunoprecipited using 10ug ab86594.
20% of IP was loaded per lane, and probed with ab86549 at 1ug/ml (lane 1) or with a control IgG (lane 2).
Detection : hemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-CENPT antibody (ab86595)

Predicted band size: 60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CENPT aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q96BT3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Centromere protein T commonly known as CENPT is an important component of the kinetochore complex. This protein has a molecular mass of approximately 94 kDa and plays an essential role in chromosome segregation during cell division. CENPT primarily localizes to the centromere region of chromosomes and is ubiquitously expressed in dividing cells particularly in human tissues with high cellular turnover. Often referred to as CENP-T it several times forms part of the inner kinetochore binding and directing microtubules for accurate chromosomal movement.

Biological function summary

Centromere protein T engages in stabilizing kinetochores ensuring proper chromosome alignment and segregation. As a part of the constitutive centromere-associated network (CCAN) complex CENPT cooperates closely with other proteins such as CENP-C and CENP-A to maintain kinetochore structure and function. This protein actively participates in facilitating the attachment of spindle microtubules to the centromere orchestrating the dynamic interactions necessary for efficient cell division.

Pathways

The role of CENPT integrates into the mechanistic regulation of the mitotic cell cycle. CENPT is pivotal in the pathway driven by the anaphase-promoting complex/cyclosome (APC/C) which regulates the separation of sister chromatids. Moreover CENPT works in conjunction with the spindle assembly checkpoint ensuring that chromosomes are properly aligned before anaphase onset. This coordination involves interactions with proteins like BUB1 and MAD2 safeguarding the fidelity of chromosome transmission during mitosis.

Centromere protein T has been implicated in chromosomal instability which is a hallmark of certain cancers. Abnormal expression or function of CENPT can lead to aneuploidy contributing to the progression of conditions such as colorectal cancer. Additionally CENPT-related defects have connections to Aurora B kinase a mitotic regulator whose dysfunction associates with cancerous phenotypes. Understanding the role of CENPT in these disorders opens avenues for therapeutic exploration targeting the mitotic machinery to combat malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.

See full target information CENPT

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 32:1241-1255 PubMed33956511

2021

Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts.

Applications

Unspecified application

Species

Unspecified reactive species

Ekaterina V Tarasovetc,Praveen Kumar Allu,Robert T Wimbish,Jennifer G DeLuca,Iain M Cheeseman,Ben E Black,Ekaterina L Grishchuk

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CENPT antibody

Western blot - Anti-CENPT antibody (AB86595)

Immunoprecipitation - Anti-CENPT antibody (AB86595)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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