Goat Polyclonal Centaurin alpha 1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADAP1 aa 350 to C-terminus.
Images
Publications
Filter by
- The Journal of cell biology 220:2021KIF13A drives AMPA receptor synaptic delivery for long-term potentiation via endosomal remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYolanda Gutiérrez,Sergio López-García,Argentina Lario,Silvia Gutiérrez-Eisman,Cédric Delevoye,José A EstebanPubMed 33999113
- Life science alliance 2:2019ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAvery Van Duzer et. al.PubMed 31792062
- The Journal of neuroscience : the official journal of the Society for Neuroscience 33:5367-742013Centaurin-α1-Ras-Elk-1 signaling at mitochondria mediates β-amyloid-induced synaptic dysfunction.Applications:WBReactive species:RatErzsebet M Szatmari,Ana F Oliveira,Elizabeth J Sumner,Ryohei YasudaPubMed 23516302
- Journal of cell science 120:2683-932007The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation.Applications:IHC-P, ICC/IF, WBReactive species:Rat, HumanCarlene D Moore,Erin E Thacker,Jennifer Larimore,David Gaston,Alison Underwood,Brian Kearns,Sean I Patterson,Trevor Jackson,Chris Chapleau,Lucas Pozzo-Miller,Anne TheibertPubMed 17635995
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ADAP1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75689
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Pig | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1-3 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Rat | Dilution info 1-3 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Pig | Dilution info 1-3 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Human | Dilution info 1-3 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).
Alternative names
CENTA1, ADAP1, Arf-GAP with dual PH domain-containing protein 1, Centaurin-alpha-1, Putative MAPK-activating protein PM25, Cnt-a1
Recommended products
Goat Polyclonal Centaurin alpha 1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADAP1 aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ADAP1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O75689
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-Centaurin alpha 1 antibody (ab27476)
Primary incubation was 1 hour. Detected by chemiluminescence.
All lanes: Western blot - Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/mL
Lane 1: Human brain tissue lysate at 35 µg
Lane 2: Mouse brain tissue lysate at 35 µg
Lane 3: Rat brain tissue lysate at 35 µg
Lane 4: Pig brain tissue lysate at 35 µg
Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centaurin alpha 1 antibody (ab27476)
IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com