AB27476

Anti-Centaurin alpha 1 antibody

BOND RX™ Validated
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(4 Publications)

Goat Polyclonal Centaurin alpha 1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ADAP1 aa 350 to C-terminus.

View Alternative Names

CENTA1, ADAP1, Arf-GAP with dual PH domain-containing protein 1, Centaurin-alpha-1, Putative MAPK-activating protein PM25, Cnt-a1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centaurin alpha 1 antibody (AB27476)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centaurin alpha 1 antibody (AB27476)

IHC image of ab27476 staining in human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab27476, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Supplier Data

Western blot - Anti-Centaurin alpha 1 antibody (AB27476)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Centaurin alpha 1 antibody (ab27476) at 1 µg/mL

Lane 1:

Human brain tissue lysate at 35 µg

Lane 2:

Mouse brain tissue lysate at 35 µg

Lane 3:

Rat brain tissue lysate at 35 µg

Lane 4:

Pig brain tissue lysate at 35 µg

Predicted band size: 42 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ADAP1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75689

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).

See full target information ADAP1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 220: PubMed33999113

2021

KIF13A drives AMPA receptor synaptic delivery for long-term potentiation via endosomal remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Yolanda Gutiérrez,Sergio López-García,Argentina Lario,Silvia Gutiérrez-Eisman,Cédric Delevoye,José A Esteban

Life science alliance 2: PubMed31792062

2019

ADAP1 promotes invasive squamous cell carcinoma progression and predicts patient survival.

Applications

Unspecified application

Species

Unspecified reactive species

Avery Van Duzer,Sachiko Taniguchi,Ajit Elhance,Takahiro Tsujikawa,Naoki Oshimori

The Journal of neuroscience : the official journal of the Society for Neuroscience 33:5367-74 PubMed23516302

2013

Centaurin-α1-Ras-Elk-1 signaling at mitochondria mediates β-amyloid-induced synaptic dysfunction.

Applications

Species

Rat

Erzsebet M Szatmari,Ana F Oliveira,Elizabeth J Sumner,Ryohei Yasuda

Journal of cell science 120:2683-93 PubMed17635995

2007

The neuronal Arf GAP centaurin alpha1 modulates dendritic differentiation.

Applications

IHC-P, ICC/IF, IHC-P, WB, ICC/IF, WB

Species

Rat, Human, Human, Human, Rat, Rat

Carlene D Moore,Erin E Thacker,Jennifer Larimore,David Gaston,Alison Underwood,Brian Kearns,Sean I Patterson,Trevor Jackson,Chris Chapleau,Lucas Pozzo-Miller,Anne Theibert

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Centaurin alpha 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centaurin alpha 1 antibody (AB27476)

Western blot - Anti-Centaurin alpha 1 antibody (AB27476)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

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