Rabbit Recombinant Monoclonal Centaurin alpha 1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ICC/IF | IP | WB | |
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Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
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Species Human | Dilution info - | Notes - |
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Species Mouse | Dilution info - | Notes - |
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Species Human | Dilution info - | Notes - |
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Species Mouse | Dilution info - | Notes - |
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Species Human | Dilution info - | Notes - |
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Species Mouse | Dilution info - | Notes - |
GTPase-activating protein for the ADP ribosylation factor family (Probable). Binds phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4).
CENTA1, ADAP1, Arf-GAP with dual PH domain-containing protein 1, Centaurin-alpha-1, Putative MAPK-activating protein PM25, Cnt-a1
Rabbit Recombinant Monoclonal Centaurin alpha 1 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ab250650 is the carrier-free version of Anti-Centaurin alpha 1 antibody [EPR14285] ab183038.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Centaurin alpha 1 antibody [EPR14285] ab183038, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of Human fetal brain lysate labeling Centaurin alpha 1 using Anti-Centaurin alpha 1 antibody [EPR14285] ab183038 at 1/50 dilution. A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.
All lanes: Immunoprecipitation - Anti-Centaurin alpha 1 antibody [EPR14285] (Anti-Centaurin alpha 1 antibody [EPR14285] ab183038)
Predicted band size: 42 kDa
This data was developed using Anti-Centaurin alpha 1 antibody [EPR14285] ab183038, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed U87 MG cells labeling Centaurin alpha 1 with Anti-Centaurin alpha 1 antibody [EPR14285] ab183038 at 1/100 followed by Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 (red). The cells are also seen stained with dapi (blue).
This data was developed using Anti-Centaurin alpha 1 antibody [EPR14285] ab183038, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Centaurin alpha 1 antibody [EPR14285] (Anti-Centaurin alpha 1 antibody [EPR14285] ab183038) at 1/20000 dilution
All lanes: Human fetal brain tissue lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 42 kDa
This data was developed using Anti-Centaurin alpha 1 antibody [EPR14285] ab183038, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Centaurin alpha 1 antibody [EPR14285] (Anti-Centaurin alpha 1 antibody [EPR14285] ab183038) at 1/5000 dilution
All lanes: A431 cell line lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 42 kDa
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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