AB101332

Anti-Centrin 1 antibody

Name: Anti-Centrin 1 antibody (ab101332) | Abcam
Brand: Abcam Limited
SKU: AB101332
Rating: 4 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal Centrin 1 antibody. Suitable for WB, ICC/IF, IP and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CETN1 aa 1 to C-terminus.

View Alternative Names

CEN1, CETN, CETN1, Centrin-1, Caltractin isoform 2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Centrin 1 antibody (AB101332)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Centrin 1 antibody (AB101332)

Immunofluorescence analysis of A431 cells fixed in ice-cold MeOH for 5 min labelling Centrin 1 protein at centrosome using ab101332 at a 1/500 dilution (Green).
Hoechst 33342 was used for nuclear staining (Blue).

Supplier Data

Immunoprecipitation - Anti-Centrin 1 antibody (AB101332)

Immunoprecipitation of Centrin-1 protein from 293T whole cell extracts using 5 µg of Centrin-1 antibody [N1C3] . Western blot analysis was performed using Centrin-1 antibody [N1C3] diluted at 1/500 dilution.

All lanes:

Immunoprecipitation - Anti-Centrin 1 antibody (ab101332) at 5 µg

Lane 1:

293T whole cell extracts

Lane 2:

293T whole cell extracts with control IgG

Lane 3:

Immunoprecipitated 293T whole cell extracts using ab101332

Secondary

All lanes:

EasyBlot anti-Rabbit IgG

Predicted band size: 20 kDa

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Unknown

Western blot - Anti-Centrin 1 antibody (AB101332)

12% SDS PAGE

All lanes:

Western blot - Anti-Centrin 1 antibody (ab101332) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 15 kDa,17 kDa,18 kDa,20 kDa,22 kDa,23 kDa,34 kDa,38 kDa,39 kDa,40 kDa

false

Supplier Data

Western blot - Anti-Centrin 1 antibody (AB101332)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Centrin 1 antibody (ab101332) at 1/1000 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human CETN1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q12798

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a fundamental role in microtubule-organizing center structure and function (PubMed : 8175926). Plays a role in sperm cilia formation (By similarity).

See full target information CETN1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0325016 PubMed40440343

2025

Two cancer cell lines utilize Myosin 10 and the kinesin HSET differentially to maintain mitotic spindle bipolarity.

Applications

Unspecified application

Species

Unspecified reactive species

Yang-In Yim,Xufeng Wu,Anjelika Gasilina,John A Hammer

Nature communications 15:9857 PubMed39543170

2024

An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Cornelia Sala,Martin Würtz,Enrico Salvatore Atorino,Annett Neuner,Patrick Partscht,Thomas Hoffmann,Sebastian Eustermann,Elmar Schiebel

Molecular biology of the cell 35:ar14 PubMed38019611

2023

Mechanisms underlying Myosin 10's contribution to the maintenance of mitotic spindle bipolarity.

Applications

Unspecified application

Species

Unspecified reactive species

Yang-In Yim,Antonio Pedrosa,Xufeng Wu,Krishna Chinthalapudi,Richard E Cheney,John A Hammer

Current biology : CB 33:912-925.e6 PubMed36720222

2023

Microtubule nucleation from the fibrous corona by LIC1-pericentrin promotes chromosome congression.

Applications

Unspecified application

Species

Unspecified reactive species

Jingchao Wu,Ainhoa Larreategui-Aparicio,Maaike L A Lambers,Dani L Bodor,Sjoerd J Klaasen,Eveline Tollenaar,Marta de Ruijter-Villani,Geert J P L Kops

The Journal of cell biology 221: PubMed36315013

2022

The central scaffold protein CEP350 coordinates centriole length, stability, and maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Onur Rojhat Karasu,Annett Neuner,Enrico Salvatore Atorino,Gislene Pereira,Elmar Schiebel

The EMBO journal 40:e106536 PubMed34009673

2021

Chromosomal instability by mutations in the novel minor spliceosome component CENATAC.

Applications

Unspecified application

Species

Unspecified reactive species

Bas de Wolf,Ali Oghabian,Maureen V Akinyi,Sandra Hanks,Eelco C Tromer,Jolien J E van Hooff,Lisa van Voorthuijsen,Laura E van Rooijen,Jens Verbeeren,Esther C H Uijttewaal,Marijke P A Baltissen,Shawn Yost,Philippe Piloquet,Michiel Vermeulen,Berend Snel,Bertrand Isidor,Nazneen Rahman,Mikko J Frilander,Geert J P L Kops

The Journal of cell biology 219: PubMed33053167

2020

Cell division requires RNA eviction from condensing chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Judith A Sharp,Carlos Perea-Resa,Wei Wang,Michael D Blower

Journal of cell science 133: PubMed32467330

2020

Transgelin-2 and phosphoregulation of the LIC2 subunit of dynein govern mitotic spindle orientation.

Applications

Unspecified application

Species

Unspecified reactive species

Amit Sharma,Sunayana Dagar,Sivaram V S Mylavarapu

eLife 6: PubMed28869035

2017

A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Muroyama,Terry Lechler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Centrin 1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Centrin 1 antibody (AB101332)

Immunoprecipitation - Anti-Centrin 1 antibody (AB101332)

Western blot - Anti-Centrin 1 antibody (AB101332)

Western blot - Anti-Centrin 1 antibody (AB101332)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (9)

Product promise