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AB156858

Anti-Centrin 1 antibody

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(1 Publication)

Rabbit Polyclonal Centrin 1 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Xenopus laevis samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CETN1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CEN1, CETN, CETN1, Centrin-1, Caltractin isoform 2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Centrin 1 antibody (AB156858)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Centrin 1 antibody (AB156858)

ab156858 staining Centrin 1 in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody at a dilution of 1/1000. DAPI (Blue) was used as a nuclear counterstain.

Western blot - Anti-Centrin 1 antibody (AB156858)
  • WB

Unknown

Western blot - Anti-Centrin 1 antibody (AB156858)

All lanes:

Western blot - Anti-Centrin 1 antibody (ab156858) at 1/1000 dilution

Lane 1:

HeLa crude cell lysate at 10 µg

Lane 2:

MCF7 crude cell lysate at 20 µg

Predicted band size: 20 kDa

false

Western blot - Anti-Centrin 1 antibody (AB156858)
  • WB

Supplier Data

Western blot - Anti-Centrin 1 antibody (AB156858)

All lanes:

Western blot - Anti-Centrin 1 antibody (ab156858) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

NIH3T3 cell lysate at 20 µg

Lane 4:

Xenopus eggs cell lysate at 50 µg

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Xenopus laevis

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human CETN1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q12798

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a fundamental role in microtubule-organizing center structure and function (PubMed : 8175926). Plays a role in sperm cilia formation (By similarity).
See full target information CETN1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Food chemistry: X 19:100806 PubMed37780314

2023

Morphological changes of mitochondria-related to apoptosis during postmortem aging of beef muscles.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmei Liu,Zhenjiang Ding,Zihan Zhang,Laiyu Zhao,Chunhui Zhang,Feng Huang
View all publications
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Product promise

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