Rabbit Polyclonal Centrin 3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CETN3.
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Publications
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- Communications biology 5:892022Planar polarity in primate cone photoreceptors: a potential role in Stiles Crawford effect phototropism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Verschueren,Leyna Boucherit,Ulisse Ferrari,Stéphane Fouquet,Céline Nouvel-Jaillard,Michel Paques,Serge Picaud,José-Alain SahelPubMed 35075261
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CETN3. The exact immunogen used to generate this antibody is proprietary information. Database link O15182
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Expected | Expected |
| Cow | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Rhesus monkey | Dilution info - | Notes - |
Target data
Function
Plays a fundamental role in microtubule-organizing center structure and function. As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores.
Alternative names
CEN3, CETN3, Centrin-3
Recommended products
Rabbit Polyclonal Centrin 3 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CETN3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CETN3. The exact immunogen used to generate this antibody is proprietary information. Database link O15182
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-Centrin 3 antibody (ab228690)
15% SDS-PAGE gel.
All lanes: Western blot - Anti-Centrin 3 antibody (ab228690) at 1/1000 dilution
Lane 1: A549 (human lung carcinoma cell line) whole cell extract at 30 µg
Lane 2: NCI-H1299 (human lung carcinoma cell line) whole cell extract at 30 µg
Lane 3: HCT 116 (human colorectal carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 20 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Centrin 3 antibody (ab228690)
Paraffin-embedded mouse cervix tissue stained for Centrin 3 using ab228690 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-Centrin 3 antibody (ab228690)
15% SDS-PAGE gel.
All lanes: Western blot - Anti-Centrin 3 antibody (ab228690) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg
Lane 2: C8D30 whole cell extract at 30 µg
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg
Lane 4: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
Lane 5: C2C12 (mouse myoblast cell line) whole cell extract at 30 µg
Lane 6: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Lane 7: Rat2 (rat fibroblast cell line) whole cell extract at 30 µg
Predicted band size: 20 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Centrin 3 antibody (ab228690)
Methanol-fixed A549 (human lung carcinoma cell line) cells stained for Centrin 3 (green) using ab228690 at 1/1000 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue). alpha Tubulin was stained with an anti-alpha Tubulin antibody (red) at 1/500 dilution.
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