Anti-CEP152 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEP152 antibody (AB183911)

Immunofluorescent analysis of HeLa cells (fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min) labeling CEP152 with ab183911 at 1/2000 dilution. Right panel costained with Hoechst 33342 (blue) and alpha 1b tubulin antibody at 1/5000 dilution (red).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEP152 antibody (AB183911)

Immunofluorescence analysis of HeLa cells fixed in MeOH labelling CEP152 protein at cytoskeleton and cytoplasm using ab183911 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody used at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).

• IP

Supplier Data

Immunoprecipitation - Anti-CEP152 antibody (AB183911)

Immunoprecipitation of CEP152 protein from HeLa whole cell extracts using 5 µg of ab183911. Western blot analysis was performed using ab183911.

All lanes:

Immunoprecipitation - Anti-CEP152 antibody (ab183911) at 5 µg

Lane 1:

HeLa whole cell extract

Lane 2:

HeLa whole cell extract with Control IgG

Lane 3:

HeLa whole cell extract with ab183911

Secondary

All lanes:

EasyBlot anti Rabbit IgG

Predicted band size: 189 kDa

false

• WB

Supplier Data

Western blot - Anti-CEP152 antibody (AB183911)

Samples were separated by 5% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-CEP152 antibody (ab183911) at 1/500 dilution

Lane 1:

293T whole cell extracts at 30 µg

Lane 2:

A431 whole cell extracts at 30 µg

Lane 3:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false