Rabbit Polyclonal CEP170 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CEP170.
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Publications
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- eLife 11:2022α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDandan Ma,Fulin Wang,Rongyi Wang,Yingchun Hu,Zhiquan Chen,Ning Huang,Yonglu Tian,Yuqing Xia,Junlin Teng,Jianguo ChenPubMed 35119360
- Journal of cell science 135:2022The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas Tischer,Jing Yang,David BarfordPubMed 34878135
- eLife 9:2020Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeng Man Chong et. al.PubMed 32242819
- Journal of cell science 132:2019A SEPT1-based scaffold is required for Golgi integrity and function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyungyeun Song et. al.PubMed 30709970
- Molecular biology of the cell 29:3105-31182018Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFelix Bärenz et. al.PubMed 30354798
- eLife 7:2018Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Ren Lin et. al.PubMed 29993362
- Nature communications 8:142572017Epstein-Barr virus particles induce centrosome amplification and chromosomal instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnatoliy Shumilov,Ming-Han Tsai,Yvonne T Schlosser,Anne-Sophie Kratz,Katharina Bernhardt,Susanne Fink,Tuba Mizani,Xiaochen Lin,Anna Jauch,Josef Mautner,Annette Kopp-Schneider,Regina Feederle,Ingrid Hoffmann,Henri-Jacques DeleclusePubMed 28186092
- Nature genetics 42:1015-202010Mutations in WDR62, encoding a centrosome-associated protein, cause microcephaly with simplified gyri and abnormal cortical architecture.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CEP170. The exact immunogen used to generate this antibody is proprietary information. Database link Q5SW79
Reactivity data
Select an application| ICC | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Chimpanzee | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 6 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Plays a role in microtubule organization (PubMed:15616186). Required for centriole subdistal appendage assembly (PubMed:28422092).
Alternative names
FAM68A, KAB, KIAA0470, CEP170, Centrosomal protein of 170 kDa, Cep170, KARP-1-binding protein, KARP1-binding protein
Recommended products
Rabbit Polyclonal CEP170 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CEP170.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CEP170. The exact immunogen used to generate this antibody is proprietary information. Database link Q5SW79
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab72505 was affinity purified using an epitope specific to CEP170 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Centrosomal Protein 170 (CEP170) also known as KIAA0470 is a significant component of centrosomal structures. This protein weighs around 170 kDa and finds widespread expression in various human tissues. At a cellular level CEP170 localizes to the spindle apparatus during mitosis where it plays a role in microtubule organization. Through its mechanical function CEP170 contributes to maintaining the structural integrity of centrosomes and helps in organizing microtubules.
- Biological function summary
CEP170 participates in cell cycle regulation and aids in the proper division of cells. It engages in a complex with other proteins at the centrosome such as CDK5RAP2 and PCM1 to ensure proper microtubule attachment and alignment during mitosis. Its interactions help to maintain centrosome cohesion and spindle formation therefore ensuring accurate chromosome segregation. Failure in these processes can lead to aneuploidy and potentially to cellular malfunction.
- Pathways
CEP170 operates within the microtubule organizing center and the cell cycle checkpoint pathways. It interacts closely with proteins like PLK1 which is important for cell cycle progression and spindle formation. Such interactions indicate its role in multiple cellular pathways that orchestrate successful division and prevent genomic instability. CEP170 also plays a part in the regulation of the p53 signaling pathway influencing cellular responses to DNA damage.
- Associated diseases and disorders
CEP170 has associations with certain cancers such as breast cancer and glioblastoma. In these contexts changes in CEP170 expression or malfunction can disrupt normal cell division therefore contributing to tumorigenesis. Its deregulation often accompanies alterations in other proteins like Aurora A kinase which may complicate normal mitotic processes. These associations indicate that CEP170's role in maintaining genomic integrity can impact disease progression and offers potential avenues for targeted therapy.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunoprecipitation - Anti-CEP170 antibody (ab72505)
CEP170 was immunoprecipitated from HeLa whole cell lysate with ab72505. Western blot was performed on the immunoprecipitate using ab72505 at 0.04μg/ml.
Lane 1 : HeLa whole cell lysate
Lane 2 : Control IgG
Chemiluminescence with an exposure time of 10 seconds.
All lanes: Immunoprecipitation - Anti-CEP170 antibody (ab72505)
Predicted band size: 175 kDa
Western blot - Anti-CEP170 antibody (ab72505)
All lanes: Western blot - Anti-CEP170 antibody (ab72505) at 0.04 µg/mL
Lane 1: HEK293T whole cell lysate at 15 µg
Lane 2: A-549 whole cell lysate at 15 µg
Lane 3: K-562 whole cell lysate at 15 µg
Lane 4: HeLa whole cell lysate at 15 µg
Predicted band size: 175 kDa
Immunocytochemistry - Anti-CEP170 antibody (ab72505)
Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling CEP170 with ab72505 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.
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