AB72505

Anti-CEP170 antibody

Name: Anti-CEP170 antibody (ab72505) | Abcam
Brand: Abcam Limited
SKU: AB72505

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(8 Publications)

Rabbit Polyclonal CEP170 antibody. Suitable for ICC, IP, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CEP170.

View Alternative Names

FAM68A, KAB, KIAA0470, CEP170, Centrosomal protein of 170 kDa, Cep170, KARP-1-binding protein, KARP1-binding protein

3 Images

Supplier Data

Immunocytochemistry - Anti-CEP170 antibody (AB72505)

Immunocytochemistry/Immunofluorescence analysis of NBF-fixed asynchronous HeLa cells labelling CEP170 with ab72505 at 1/200. A red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.

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Immunoprecipitation - Anti-CEP170 antibody (AB72505)

CEP170 was immunoprecipitated from HeLa whole cell lysate with ab72505. Western blot was performed on the immunoprecipitate using ab72505 at 0.04μg/ml.

Lane 1 : HeLa whole cell lysate

Lane 2 : Control IgG

Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-CEP170 antibody (ab72505)

Predicted band size: 175 kDa

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Western blot - Anti-CEP170 antibody (AB72505)

All lanes:

Western blot - Anti-CEP170 antibody (ab72505) at 0.04 µg/mL

Lane 1:

HEK293T whole cell lysate at 15 µg

Lane 2:

A-549 whole cell lysate at 15 µg

Lane 3:

K-562 whole cell lysate at 15 µg

Lane 4:

HeLa whole cell lysate at 15 µg

Predicted band size: 175 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IP, WB

applications

Immunogen

Synthetic Peptide within Human CEP170. The exact immunogen used to generate this antibody is proprietary information.

Q5SW79

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "6 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04 µg/mL", "WB-species-notes": "" }, "Chimpanzee": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab72505 was affinity purified using an epitope specific to CEP170 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Centrosomal Protein 170 (CEP170) also known as KIAA0470 is a significant component of centrosomal structures. This protein weighs around 170 kDa and finds widespread expression in various human tissues. At a cellular level CEP170 localizes to the spindle apparatus during mitosis where it plays a role in microtubule organization. Through its mechanical function CEP170 contributes to maintaining the structural integrity of centrosomes and helps in organizing microtubules.

Biological function summary

CEP170 participates in cell cycle regulation and aids in the proper division of cells. It engages in a complex with other proteins at the centrosome such as CDK5RAP2 and PCM1 to ensure proper microtubule attachment and alignment during mitosis. Its interactions help to maintain centrosome cohesion and spindle formation therefore ensuring accurate chromosome segregation. Failure in these processes can lead to aneuploidy and potentially to cellular malfunction.

Pathways

CEP170 operates within the microtubule organizing center and the cell cycle checkpoint pathways. It interacts closely with proteins like PLK1 which is important for cell cycle progression and spindle formation. Such interactions indicate its role in multiple cellular pathways that orchestrate successful division and prevent genomic instability. CEP170 also plays a part in the regulation of the p53 signaling pathway influencing cellular responses to DNA damage.

CEP170 has associations with certain cancers such as breast cancer and glioblastoma. In these contexts changes in CEP170 expression or malfunction can disrupt normal cell division therefore contributing to tumorigenesis. Its deregulation often accompanies alterations in other proteins like Aurora A kinase which may complicate normal mitotic processes. These associations indicate that CEP170's role in maintaining genomic integrity can impact disease progression and offers potential avenues for targeted therapy.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a role in microtubule organization (PubMed : 15616186). Required for centriole subdistal appendage assembly (PubMed : 28422092).

See full target information CEP170

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35119360

2022

α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Ma,Fulin Wang,Rongyi Wang,Yingchun Hu,Zhiquan Chen,Ning Huang,Yonglu Tian,Yuqing Xia,Junlin Teng,Jianguo Chen

Journal of cell science 135: PubMed34878135

2022

The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Tischer,Jing Yang,David Barford

eLife 9: PubMed32242819

2020

Super-resolution microscopy reveals coupling between mammalian centriole subdistal appendages and distal appendages.

Applications

Unspecified application

Species

Unspecified reactive species

Weng Man Chong,Won-Jing Wang,Chien-Hui Lo,Tzu-Yuan Chiu,Ting-Jui Chang,You-Pi Liu,Barbara Tanos,Gregory Mazo,Meng-Fu Bryan Tsou,Wann-Neng Jane,T Tony Yang,Jung-Chi Liao

Journal of cell science 132: PubMed30709970

2019

A SEPT1-based scaffold is required for Golgi integrity and function.

Applications

Unspecified application

Species

Unspecified reactive species

Kyungyeun Song,Claudia Gras,Gabrielle Capin,Niclas Gimber,Martin Lehmann,Saif Mohd,Dmytro Puchkov,Maria Rödiger,Ilka Wilhelmi,Oliver Daumke,Jan Schmoranzer,Annette Schürmann,Michael Krauss

Molecular biology of the cell 29:3105-3118 PubMed30354798

2018

Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Bärenz,Yvonne T Kschonsak,Annalena Meyer,Aliakbar Jafarpour,Holger Lorenz,Ingrid Hoffmann

eLife 7: PubMed29993362

2018

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ren Lin,Benjamin Izar,Shu Wang,Clarence Yapp,Shaolin Mei,Parin M Shah,Sandro Santagata,Peter K Sorger

Nature communications 8:14257 PubMed28186092

2017

Epstein-Barr virus particles induce centrosome amplification and chromosomal instability.

Applications

Unspecified application

Species

Unspecified reactive species

Anatoliy Shumilov,Ming-Han Tsai,Yvonne T Schlosser,Anne-Sophie Kratz,Katharina Bernhardt,Susanne Fink,Tuba Mizani,Xiaochen Lin,Anna Jauch,Josef Mautner,Annette Kopp-Schneider,Regina Feederle,Ingrid Hoffmann,Henri-Jacques Delecluse

Nature genetics 42:1015-20 PubMed20890278

2010

Mutations in WDR62, encoding a centrosome-associated protein, cause microcephaly with simplified gyri and abnormal cortical architecture.

Applications

ICC/IF

Species

Human

Timothy W Yu,Ganeshwaran H Mochida,David J Tischfield,Sema K Sgaier,Laura Flores-Sarnat,Consolato M Sergi,Meral Topçu,Marie T McDonald,Brenda J Barry,Jillian M Felie,Christine Sunu,William B Dobyns,Rebecca D Folkerth,A James Barkovich,Christopher A Walsh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CEP170 antibody

Immunocytochemistry - Anti-CEP170 antibody (AB72505)

Immunoprecipitation - Anti-CEP170 antibody (AB72505)

Western blot - Anti-CEP170 antibody (AB72505)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise