AB84870

Anti-CEP290 antibody

Name: Anti-CEP290 antibody (ab84870) | Abcam
Brand: Abcam Limited
SKU: AB84870
Price: 565 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(36 Publications)

Rabbit Polyclonal CEP290 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human CEP290 aa 2400 to C-terminus.

View Alternative Names

BBS14, KIAA0373, NPHP6, CEP290, Centrosomal protein of 290 kDa, Cep290, Bardet-Biedl syndrome 14 protein, Cancer/testis antigen 87, Nephrocystin-6, Tumor antigen se2-2, CT87

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CEP290 antibody (AB84870)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CEP290 antibody (AB84870)

Staining of human CEP290 in NBF fixed asynchronous HeLa cells by Immunocytochemistry, using ab84870 at a dilution of 1/250. Detection : Red fluorescent goat anti-rabbit IgG used at a dilution of 1/100.

Lab

Western blot - Anti-CEP290 antibody (AB84870)

Western blot : Anti-CEP290 antibody (ab84870) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab84870 was shown to bind specifically to CEP290. A band was observed at 290 kDa in wild-type A549 cell lysates with no signal observed at this size in CEP290 knockout cell line. To generate this image, wild-type and CEP290 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CEP290 antibody (ab84870) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

CEP290 knockout A549 cell lysate at 20 µg

Lane 3:

Wild-type A549 ab288558 cell lysate at 10 µg

Lane 4:

CEP290 knockout A549 STN-436528 cell lysate at 40 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 290 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CEP290 aa 2400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15078

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p>Fix with formaldehyde. Acetone fixation is not recommended.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Elephant": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified using an epitope specific to CEP290 immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEP290 also known as centrosomal protein of 290 kDa acts as an important structural component in cells. It has an approximate mass of 290 kilodaltons and is primarily expressed in ciliated cells across various tissues including the retina and kidneys. CEP290 localizes at the centrosome and is integral to the formation and stability of cilia and flagella. The protein plays an important mechanic role in maintaining the structural integrity of cilia which are essential for cellular signaling and motility.

Biological function summary

CEP290 contributes significantly to the formation and maintenance of the primary cilium a cellular organelle. It forms part of the transition zone complex a critical region at the base of cilia. This zone functions as a barrier regulating the movement of molecules in and out of the cilia. As a result CEP290 supports the transport and localization of ciliary proteins affecting ciliary homeostasis and signal transduction mechanisms within the cell.

Pathways

CEP290 is intricately involved in the ciliary transport and the phototransduction pathway within photoreceptor cells. CEP290 operates in coordination with proteins such as RPGR in these pathways ensuring proper ciliary trafficking and function. This activity is important for sensory perception processes including vision. The disruption of CEP290 affects these pathways leading to compromised sensory functions.

CEP290 mutations associate with specific ciliopathies such as Joubert syndrome and Leber congenital amaurosis. These conditions result from defective ciliary function and altered cellular pathways. In the context of Joubert syndrome CEP290 interacts with proteins such as AHI1 where both play roles in neurodevelopment and function. The understanding of these interactions provides insights into the mechanistic implications of CEP290-related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in early and late steps in cilia formation. Its association with CCP110 is required for inhibition of primary cilia formation by CCP110 (PubMed : 18694559). May play a role in early ciliogenesis in the disappearance of centriolar satellites and in the transition of primary ciliar vesicles (PCVs) to capped ciliary vesicles (CCVs). Required for the centrosomal recruitment of RAB8A and for the targeting of centriole satellite proteins to centrosomes such as of PCM1 (PubMed : 24421332). Required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes (By similarity). Required for efficient recruitment of RAB8A to primary cilium (PubMed : 17705300). In the ciliary transition zone is part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (By similarity). Involved in regulation of the BBSome complex integrity, specifically for presence of BBS2, BBS5 and BBS8/TTC8 in the complex, and in ciliary targeting of selected BBSome cargos. May play a role in controlling entry of the BBSome complex to cilia possibly implicating IQCB1/NPHP5 (PubMed : 25552655). Activates ATF4-mediated transcription (PubMed : 16682973).

See full target information CEP290

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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:911 PubMed37670137

2023

INPP5E regulates CD3ζ enrichment at the immune synapse by phosphoinositide distribution control.

Applications

Unspecified application

Species

Unspecified reactive species

Tzu-Yuan Chiu,Chien-Hui Lo,Yi-Hsuan Lin,Yun-Di Lai,Shan-Shan Lin,Ya-Tian Fang,Wei-Syun Huang,Shen-Yan Huang,Pei-Yuan Tsai,Fu-Hua Yang,Weng Man Chong,Yi-Chieh Wu,Hsing-Chen Tsai,Ya-Wen Liu,Chia-Lin Hsu,Jung-Chi Liao,Won-Jing Wang

Cells 12: PubMed37371046

2023

Eupatilin Improves Cilia Defects in Human CEP290 Ciliopathy Models.

Applications

Unspecified application

Species

Unspecified reactive species

Julio C Corral-Serrano,Paul E Sladen,Daniele Ottaviani,Olivia F Rezek,Dimitra Athanasiou,Katarina Jovanovic,Jacqueline van der Spuy,Brian C Mansfield,Michael E Cheetham

Disease models & mechanisms 15: PubMed36533556

2022

Variable phenotypes and penetrance between and within different zebrafish ciliary transition zone mutants.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Holly R Thomas,Robert G Thompson,Stephanie C Waldrep,Joseph Fogerty,Ping Song,Zhang Li,Yongjie Ma,Peu Santra,Jonathan D Hoover,Nan Cher Yeo,Iain A Drummond,Bradley K Yoder,Jeffrey D Amack,Brian Perkins,John M Parant

EMBO reports 24:e54935 PubMed36314725

2022

Actin filaments form a size-dependent diffusion barrier around centrosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hsuan Cheng,Yu-Lin Kao,Ting Chen,Lohitaksh Sharma,Wen-Ting Yang,Yi-Chien Chuang,Shih-Han Huang,Hong-Rui Lin,Yao-Shen Huang,Chi-Ling Kao,Lee-Wei Yang,Rachel Bearon,Hui-Chun Cheng,Kuo-Chiang Hsia,Yu-Chun Lin

Science advances 7: PubMed34162535

2021

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Chen,Qingwen Xu,Yuxia Zhang,Brian A Davies,Yan Huang,David J Katzmann,Peter C Harris,Jinghua Hu,Kun Ling

Methods in molecular biology (Clifton, N.J.) 2329:249-263 PubMed34085228

2021

Analyzing Centrioles and Cilia by Expansion Microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Kong,Jadranka Loncarek

The Journal of cell biology 220: PubMed33983387

2021

TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity.

Applications

Unspecified application

Species

Unspecified reactive species

Franz Meitinger,Dong Kong,Midori Ohta,Arshad Desai,Karen Oegema,Jadranka Loncarek

Molecular biology of the cell 32:675-689 PubMed33625872

2021

Rpgrip1l controls ciliary gating by ensuring the proper amount of Cep290 at the vertebrate transition zone.

Applications

Unspecified application

Species

Unspecified reactive species

Antonia Wiegering,Renate Dildrop,Christine Vesque,Hemant Khanna,Sylvie Schneider-Maunoury,Christoph Gerhardt

Stem cell research 52:102243 PubMed33626495

2021

Knockout of the CEP290 gene in human induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph Fogerty,Brian D Perkins

PLoS biology 18:e3000679 PubMed32555591

2020

Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.

Applications

Unspecified application

Species

Unspecified reactive species

Özge Z Aydin,Sevket Onur Taflan,Can Gurkaslar,Elif Nur Firat-Karalar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CEP290 antibody

Immunocytochemistry/ Immunofluorescence - Anti-CEP290 antibody (AB84870)

Western blot - Anti-CEP290 antibody (AB84870)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (36)

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