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Rabbit Recombinant Monoclonal CEP290 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse samples.

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Images

Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (AB318279), expandable thumbnail
  • Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (AB318279), expandable thumbnail
  • Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (AB318279), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (AB318279), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Dog
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Dog, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Dog, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Dog
Dilution info
-
Notes

-

Target data

Function

Involved in early and late steps in cilia formation. Its association with CCP110 is required for inhibition of primary cilia formation by CCP110 (PubMed:18694559). May play a role in early ciliogenesis in the disappearance of centriolar satellites and in the transition of primary ciliar vesicles (PCVs) to capped ciliary vesicles (CCVs). Required for the centrosomal recruitment of RAB8A and for the targeting of centriole satellite proteins to centrosomes such as of PCM1 (PubMed:24421332). Required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes (By similarity). Required for efficient recruitment of RAB8A to primary cilium (PubMed:17705300). In the ciliary transition zone is part of the tectonic-like complex which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (By similarity). Involved in regulation of the BBSome complex integrity, specifically for presence of BBS2, BBS5 and BBS8/TTC8 in the complex, and in ciliary targeting of selected BBSome cargos. May play a role in controlling entry of the BBSome complex to cilia possibly implicating IQCB1/NPHP5 (PubMed:25552655). Activates ATF4-mediated transcription (PubMed:16682973).

Alternative names

BBS14, KIAA0373, NPHP6, CEP290, Centrosomal protein of 290 kDa, Cep290, Bardet-Biedl syndrome 14 protein, Cancer/testis antigen 87, Nephrocystin-6, Tumor antigen se2-2, CT87

Recommended products

Rabbit Recombinant Monoclonal CEP290 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28924-76
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for mouse ICC

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab318279 is the carrier-free version of Anti-CEP290 antibody [EPR28924-76] ab318278.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CEP290 also known as centrosomal protein of 290 kDa acts as an important structural component in cells. It has an approximate mass of 290 kilodaltons and is primarily expressed in ciliated cells across various tissues including the retina and kidneys. CEP290 localizes at the centrosome and is integral to the formation and stability of cilia and flagella. The protein plays an important mechanic role in maintaining the structural integrity of cilia which are essential for cellular signaling and motility.

Biological function summary

CEP290 contributes significantly to the formation and maintenance of the primary cilium a cellular organelle. It forms part of the transition zone complex a critical region at the base of cilia. This zone functions as a barrier regulating the movement of molecules in and out of the cilia. As a result CEP290 supports the transport and localization of ciliary proteins affecting ciliary homeostasis and signal transduction mechanisms within the cell.

Pathways

CEP290 is intricately involved in the ciliary transport and the phototransduction pathway within photoreceptor cells. CEP290 operates in coordination with proteins such as RPGR in these pathways ensuring proper ciliary trafficking and function. This activity is important for sensory perception processes including vision. The disruption of CEP290 affects these pathways leading to compromised sensory functions.

Associated diseases and disorders

CEP290 mutations associate with specific ciliopathies such as Joubert syndrome and Leber congenital amaurosis. These conditions result from defective ciliary function and altered cellular pathways. In the context of Joubert syndrome CEP290 interacts with proteins such as AHI1 where both play roles in neurodevelopment and function. The understanding of these interactions provides insights into the mechanistic implications of CEP290-related disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (ab318279), expandable thumbnail

    Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (ab318279)

    CEP290 Western blot staining using rabbit Anti-CEP290 antibody

    This data was developed using Anti-CEP290 antibody [EPR28924-76] ab318278, the same antibody clone in a different buffer formulation.

    The lane 4 was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    Exposure time: Lanes 1-3: 92 seconds, lane 4: 180 seconds

    All lanes: Western blot - Anti-CEP290 antibody [EPR28924-76] (Anti-CEP290 antibody [EPR28924-76] ab318278) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HeLa transfected with siRNA specifically targeting CEP290 whole cell lysate at 20 µg with NFDM/TBST

    Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg with NFDM/TBST

    Lane 4: Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 40 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 290 kDa, 124 kDa

  • Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (ab318279), expandable thumbnail

    Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (ab318279)

    CEP290 Western blot staining using rabbit Anti-CEP290 antibody

    The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

    This data was developed using Anti-CEP290 antibody [EPR28924-76] ab318278, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-CEP290 antibody [EPR28924-76] (Anti-CEP290 antibody [EPR28924-76] ab318278) at 1/1000 dilution

    Lane 1: Human placenta tissue lysate at 50 µg with NFDM/TBST

    Lane 2: Human adrenal gland tissue lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Developed using the ECL technique.

    Observed band size: 290 kDa, 124 kDa

    Exposure time: 180s

  • Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (ab318279), expandable thumbnail

    Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (ab318279)

    CEP290 Western blot staining using rabbit Anti-CEP290 antibody

    The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

    This data was developed using Anti-CEP290 antibody [EPR28924-76] ab318278, the same antibody clone in a different buffer formulation.

    Low expression: skeletal muscle.

    The identity of the bands between 130 kDa and 250 kDa are unknown.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-CEP290 antibody [EPR28924-76] (Anti-CEP290 antibody [EPR28924-76] ab318278) at 1/1000 dilution

    Lane 1: Mouse olfactory bulb tissue lysate at 50 µg with NFDM/TBST

    Lane 2: Mouse brain tissue lysate at 50 µg with NFDM/TBST

    Lane 3: Mouse skeletal muscle lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 290 kDa, 124 kDa

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (ab318279), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (ab318279)

    This data was developed using Anti-CEP290 antibody [EPR28924-76] ab318278, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling CEP290 with Anti-CEP290 antibody [EPR28924-76] ab318278 at 1/50 (10.38 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing positive staining on the centrosome in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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