Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (AB318279)

This data was developed using ab318278, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling CEP290 with ab318278 at 1/50 (10.38 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

Confocal image showing positive staining on the centrosome in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• WB

Supplier Data

Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (AB318279)

The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

This data was developed using ab318278, the same antibody clone in a different buffer formulation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-CEP290 antibody [EPR28924-76] (<a href='/en-us/products/primary-antibodies/cep290-antibody-epr28924-76-ab318278'>ab318278</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 50 µg

Lane 2:

Human adrenal gland tissue lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 290 kDa,124 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (AB318279)

This data was developed using ab318278, the same antibody clone in a different buffer formulation.

The lane 4 was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lanes 1-3 : 92 seconds, lane 4 : 180 seconds

All lanes:

Western blot - Anti-CEP290 antibody [EPR28924-76] (<a href='/en-us/products/primary-antibodies/cep290-antibody-epr28924-76-ab318278'>ab318278</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting CEP290 whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 40 µg

Lane 4:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 290 kDa,124 kDa

false

• WB

Supplier Data

Western blot - Anti-CEP290 antibody [EPR28924-76] - BSA and Azide free (AB318279)

The high-sensitivity ECL substrate allows for the detection of proteins in the mid-femtogram range.

This data was developed using ab318278, the same antibody clone in a different buffer formulation.

Low expression : skeletal muscle.

The identity of the bands between 130 kDa and 250 kDa are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-CEP290 antibody [EPR28924-76] (<a href='/en-us/products/primary-antibodies/cep290-antibody-epr28924-76-ab318278'>ab318278</a>) at 1/1000 dilution

Lane 1:

Mouse olfactory bulb tissue lysate at 50 µg

Lane 2:

Mouse brain tissue lysate at 50 µg

Lane 3:

Mouse skeletal muscle lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 290 kDa,124 kDa

true

Exposure time: 180s