Rabbit Polyclonal CEP295 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CEP295 aa 1850-2000.
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Publications
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- Nature communications 11:9032020CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnrico S Atorino et. al.PubMed 32060285
- Journal of cell science 132:2019PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaeyoun Kim et. al.PubMed 30814333
- Molecular biology of the cell 29:2801-28082018PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChii Shyang Fong et. al.PubMed 30230954
- eLife 7:2018PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew Michael Sydor et. al.PubMed 30168418
- Cell death & disease 9:5712018Caspase-mediated cleavage of the centrosomal proteins during apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMi Young Seo et. al.PubMed 29752437
- Nature cell biology 18:87-992015Conserved molecular interactions in centriole-to-centrosome conversion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingyan Fu,Zoltan Lipinszki,Hélène Rangone,Mingwei Min,Charlotte Mykura,Jennifer Chao-Chu,Sandra Schneider,Nikola S Dzhindzhev,Marco Gottardo,Maria Giovanna Riparbelli,Giuliano Callaini,David M GloverPubMed 26595382
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CEP295 aa 1850-2000. The exact immunogen used to generate this antibody is proprietary information. Database link Q9C0D2
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250.00000 - 1/500.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Centriole-enriched microtubule-binding protein involved in centriole biogenesis (PubMed:20844083, PubMed:25131205, PubMed:27185865, PubMed:38154379). Essential for the generation of the distal portion of new-born centrioles in a CENPJ- and CEP120-mediated elongation dependent manner during the cell cycle S/G2 phase after formation of the initiating cartwheel structure (PubMed:27185865). Required for the recruitment of centriolar proteins, such as POC1B, POC5 and CEP135, into the distal portion of centrioles (PubMed:27185865). Also required for centriole-to-centrosome conversion during mitotic progression, but is dispensable for cartwheel removal or centriole disengagement (PubMed:25131205). Binds to and stabilizes centriolar microtubule (PubMed:27185865). May be involved in ciliogenesis (PubMed:38154379).
Alternative names
KIAA1731, KIAA1731, CEP295, Centrosomal protein of 295 kDa
Recommended products
Rabbit Polyclonal CEP295 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CEP295 aa 1850-2000.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CEP295 aa 1850-2000. The exact immunogen used to generate this antibody is proprietary information. Database link Q9C0D2
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Avoid freeze / thaw cycle, Store undiluted
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CEP295 also known as centrosomal protein 295 plays a critical role in centrosome function and dynamics. This protein has a molecular mass of approximately 294 kDa. CEP295 localizes at the centrosome and is heavily expressed in tissues with rapid cell division such as the brain and testes. The protein is important for proper centrosomal structure and allows for the recruitment of other centrosomal components necessary for its function.
- Biological function summary
CEP295 participates in the organization and stability of centrosomes ensuring correct spindle pole formation during mitosis. The protein functions as part of a larger centrosomal complex that includes other essential structural proteins contributing to the centrosomal microtubule-nucleating activity. By facilitating centrosome cohesion CEP295 supports proper chromosome segregation and cell cycle progression.
- Pathways
CEP295 engages in critical cellular processes such as the cell cycle and mitotic spindle assembly pathways. It interacts closely with proteins like PLK4 and CEP152 both involved in centrosome duplication and mitotic progression. Through these pathways CEP295 ensures that centrosomes duplicate only once per cycle maintaining genomic stability and preventing aneuploidy.
- Associated diseases and disorders
Improper functioning of CEP295 links to conditions such as microcephaly and certain cancers. The disruption of CEP295 function can lead to defective centrosomes giving rise to abnormal cell divisions that are implicated in these disorders. Proteins like CEP152 also exhibit association with microcephaly emphasizing the importance of centrosome integrity for proper neural development and cell proliferation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-CEP295 antibody (ab122490)
Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labeling CEP295 with ab12249 shows positivity in the plasma membrane and cytoplasm.
Western blot - Anti-CEP295 antibody (ab122490)
All lanes: Western blot - Anti-CEP295 antibody (ab122490) at 1/250 dilution
Lane 1: RT 4 cell lysate
Lane 2: U 251 MG cell lysate
Lane 3: Human Plasma lysate
Lane 4: Human Liver lysate
Lane 5: Human Tonsil lysate
Developed using the ECL technique.
Predicted band size: 295 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP295 antibody (ab122490)
ab122490 at 1/200 dilution staining CEP295 in paraffin-embedded human prostate tissue by Immunohistochemistry.
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