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AB122490

Anti-CEP295 antibody

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(7 Publications)

Rabbit Polyclonal CEP295 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CEP295 aa 1850-2000.

View Alternative Names

KIAA1731, CEP295, Centrosomal protein of 295 kDa

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CEP295 antibody (AB122490)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEP295 antibody (AB122490)

Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labeling CEP295 with ab12249 shows positivity in the plasma membrane and cytoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP295 antibody (AB122490)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP295 antibody (AB122490)

ab122490 at 1/200 dilution staining CEP295 in paraffin-embedded human prostate tissue by Immunohistochemistry.

Western blot - Anti-CEP295 antibody (AB122490)
  • WB

Unknown

Western blot - Anti-CEP295 antibody (AB122490)

All lanes:

Western blot - Anti-CEP295 antibody (ab122490) at 1/250 dilution

Lane 1:

RT 4 cell lysate

Lane 2:

U 251 MG cell lysate

Lane 3:

Human Plasma lysate

Lane 4:

Human Liver lysate

Lane 5:

Human Tonsil lysate

Predicted band size: 295 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CEP295 aa 1850-2000. The exact immunogen used to generate this antibody is proprietary information.

Q9C0D2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|Store undiluted
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEP295 also known as centrosomal protein 295 plays a critical role in centrosome function and dynamics. This protein has a molecular mass of approximately 294 kDa. CEP295 localizes at the centrosome and is heavily expressed in tissues with rapid cell division such as the brain and testes. The protein is important for proper centrosomal structure and allows for the recruitment of other centrosomal components necessary for its function.
Biological function summary

CEP295 participates in the organization and stability of centrosomes ensuring correct spindle pole formation during mitosis. The protein functions as part of a larger centrosomal complex that includes other essential structural proteins contributing to the centrosomal microtubule-nucleating activity. By facilitating centrosome cohesion CEP295 supports proper chromosome segregation and cell cycle progression.

Pathways

CEP295 engages in critical cellular processes such as the cell cycle and mitotic spindle assembly pathways. It interacts closely with proteins like PLK4 and CEP152 both involved in centrosome duplication and mitotic progression. Through these pathways CEP295 ensures that centrosomes duplicate only once per cycle maintaining genomic stability and preventing aneuploidy.

Improper functioning of CEP295 links to conditions such as microcephaly and certain cancers. The disruption of CEP295 function can lead to defective centrosomes giving rise to abnormal cell divisions that are implicated in these disorders. Proteins like CEP152 also exhibit association with microcephaly emphasizing the importance of centrosome integrity for proper neural development and cell proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Centriole-enriched microtubule-binding protein involved in centriole biogenesis (PubMed : 20844083, PubMed : 25131205, PubMed : 27185865, PubMed : 38154379). Essential for the generation of the distal portion of new-born centrioles in a CENPJ- and CEP120-mediated elongation dependent manner during the cell cycle S/G2 phase after formation of the initiating cartwheel structure (PubMed : 27185865). Required for the recruitment of centriolar proteins, such as POC1B, POC5 and CEP135, into the distal portion of centrioles (PubMed : 27185865). Also required for centriole-to-centrosome conversion during mitotic progression, but is dispensable for cartwheel removal or centriole disengagement (PubMed : 25131205). Binds to and stabilizes centriolar microtubule (PubMed : 27185865). May be involved in ciliogenesis (PubMed : 38154379).
See full target information CEP295
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9857 PubMed39543170

2024

An interaction network of inner centriole proteins organised by POC1A-POC1B heterodimer crosslinks ensures centriolar integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Cornelia Sala,Martin Würtz,Enrico Salvatore Atorino,Annett Neuner,Patrick Partscht,Thomas Hoffmann,Sebastian Eustermann,Elmar Schiebel

Nature communications 11:903 PubMed32060285

2020

CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Enrico S Atorino,Shoji Hata,Charlotta Funaya,Annett Neuner,Elmar Schiebel

Journal of cell science 132: PubMed30814333

2019

PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jaeyoun Kim,Jeongjin Kim,Kunsoo Rhee

Molecular biology of the cell 29:2801-2808 PubMed30230954

2018

PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Chii Shyang Fong,Kanako Ozaki,Meng-Fu Bryan Tsou

eLife 7: PubMed30168418

2018

PPP1R35 is a novel centrosomal protein that regulates centriole length in concert with the microcephaly protein RTTN.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Michael Sydor,Etienne Coyaud,Cristina Rovelli,Estelle Laurent,Helen Liu,Brian Raught,Vito Mennella

Cell death & disease 9:571 PubMed29752437

2018

Caspase-mediated cleavage of the centrosomal proteins during apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mi Young Seo,Kunsoo Rhee

Nature cell biology 18:87-99 PubMed26595382

2015

Conserved molecular interactions in centriole-to-centrosome conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyan Fu,Zoltan Lipinszki,Hélène Rangone,Mingwei Min,Charlotte Mykura,Jennifer Chao-Chu,Sandra Schneider,Nikola S Dzhindzhev,Marco Gottardo,Maria Giovanna Riparbelli,Giuliano Callaini,David M Glover
View all publications
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