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AB220093

Anti-CEP350 antibody

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(1 Publication)

Rabbit Polyclonal CEP350 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CEP350 aa 1400-1550.

View Alternative Names

CAP350, KIAA0480, GM133, CEP350, Centrosome-associated protein 350, Cep350, Centrosome-associated protein of 350 kDa

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-CEP350 antibody (AB220093)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEP350 antibody (AB220093)

Immunofluorescent analysis of PFA fixed, Triton X-100 permeabilized U-251 MG cells labeling CEP350 with ab220093 at 4 μg/ml (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CEP350 aa 1400-1550. The exact immunogen used to generate this antibody is proprietary information.

Q5VT06

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEP350 also known as Centrosome-associated protein 350 is a large protein weighing approximately 350 kDa. It plays an important mechanical role in centrosome structure and function. Expressed mainly in tissues with high proliferative capacity CEP350 anchors microtubules to the centrosomes ensuring proper cell division. The protein localizes to the centrosome and is important for maintaining microtubule organization.
Biological function summary

CEP350 interacts with other centrosomal components to ensure proper spindle formation during mitosis. It is part of the pericentriolar material participating in complex formation with proteins such as ninein and AKAP450. These interactions stabilize the centrosome enabling effective microtubule nucleation and anchoring which are essential for cell cycle progression and cellular integrity.

Pathways

CEP350 contributes to the centrosome cycle and microtubule dynamics regulation. It plays a role in the interphase microtubule pathway coordinating with proteins like pericentrin to manage microtubule organization and stability. CEP350's interaction with the AKAP450 protein complex influences the microtubule-organizing center activity reflecting its role in essential cellular pathways.

CEP350 mutations or dysregulation link to microcephaly and retinal degeneration highlighting its role in neural development and visual function. Its association with pericentrin and AKAP450 can also impact ciliogenesis which when impaired may contribute to ciliopathies. Understanding CEP350's involvement in these conditions could lead to insights into their molecular mechanisms and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an essential role in centriole growth by stabilizing a procentriolar seed composed of at least, SASS6 and CENPJ (PubMed : 19052644). Required for anchoring microtubules to the centrosomes and for the integrity of the microtubule network (PubMed : 16314388, PubMed : 17878239, PubMed : 28659385). Recruits PPARA to discrete subcellular compartments and thereby modulates PPARA activity (PubMed : 15615782). Required for ciliation (PubMed : 28659385).
See full target information CEP350
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 17:23545-23567 PubMed37988765

2023

Single-Component Multilayered Self-Assembling Protein Nanoparticles Displaying Extracellular Domains of Matrix Protein 2 as a Pan-influenza A Vaccine.

Applications

Unspecified application

Species

Unspecified reactive species

Keegan Braz Gomes,Yi-Nan Zhang,Yi-Zong Lee,Mor Eldad,Alexander Lim,Garrett Ward,Sarah Auclair,Linling He,Jiang Zhu
View all publications
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