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AB169301

Anti-CEP57 antibody

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(3 Publications)

Mouse Polyclonal CEP57 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CEP57.

View Alternative Names

KIAA0092, TSP57, CEP57, Centrosomal protein of 57 kDa, Cep57, FGF2-interacting protein, Testis-specific protein 57, Translokin

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CEP57 antibody (AB169301)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CEP57 antibody (AB169301)

Immunofluorescent analysis of HeLa cells labeling CEP57 with ab169301 at 10 μg/ml.

Western blot - Anti-CEP57 antibody (AB169301)
  • WB

Unknown

Western blot - Anti-CEP57 antibody (AB169301)

All lanes:

Western blot - Anti-CEP57 antibody (ab169301) at 1 µg/mL

Lane 1:

CEP57-transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 57 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CEP57.

Q86XR8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEP57 also known as Centrosomal Protein 57 or Translokin is a protein with a molecular mass of approximately 57 kDa. CEP57 plays an important mechanical role in the centrosome which is important for the organization of microtubules during cell division. This protein localizes in the centrosome area within the cell cytoplasm. Expression of CEP57 is widespread occurring in various tissues which suggests its function is significant in different cellular contexts.
Biological function summary

CEP57 is important for proper cell division and spindle pole formation. It interacts within a complex of centrosomal proteins playing roles in microtubule nucleation and stabilization. CEP57 also functions as a microtubule-binding protein aiding the accurate segregation of chromosomes during mitosis. By stabilizing microtubule-kinetochore attachments CEP57 ensures effective chromosomal distribution hence maintaining genomic integrity and preventing potential cellular abnormalities.

Pathways

CEP57 contributes significantly to the cell cycle and mitotic spindle organization pathways. It works alongside other centrosomal proteins such as CEP192 and γ-tubulin enhancing their interaction and stabilization of microtubules. CEP57 is also linked to the microtubule-organizing center integrating into pathways important for cell division and duplication processes which are central to maintaining cellular homeostasis and function.

Researchers have associated CEP57 with certain cancers and mosaic variegated aneuploidy syndrome. In cancer overexpression or altered function of CEP57 might lead to improper cell division contributing to tumorigenesis. Furthermore in mosaic variegated aneuploidy syndrome improper function of CEP57 leads to chromosomal instability and abnormal cell growth. CEP57 involvement in these diseases highlights its interactions with other proteins such as TP53 which also manages cell cycle regulation and genomic stability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Centrosomal protein which may be required for microtubule attachment to centrosomes. May act by forming ring-like structures around microtubules. Mediates nuclear translocation and mitogenic activity of the internalized growth factor FGF2, but that of FGF1.
See full target information CEP57
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 44:1294-1321 PubMed39905228

2025

Multimodal mechanisms of human centriole engagement and disengagement.

Applications

Unspecified application

Species

Unspecified reactive species

Kei K Ito,Kasuga Takumi,Kyohei Matsuhashi,Hirokazu Sakamoto,Kaho Nagai,Masamitsu Fukuyama,Shohei Yamamoto,Takumi Chinen,Shoji Hata,Daiju Kitagawa

The Journal of cell biology 220: PubMed33492359

2021

Cep57 and Cep57L1 maintain centriole engagement in interphase to ensure centriole duplication cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Kei K Ito,Koki Watanabe,Haruki Ishida,Kyohei Matsuhashi,Takumi Chinen,Shoji Hata,Daiju Kitagawa

Nature communications 10:931 PubMed30804344

2019

The Cep57-pericentrin module organizes PCM expansion and centriole engagement.

Applications

Unspecified application

Species

Unspecified reactive species

Koki Watanabe,Daisuke Takao,Kei K Ito,Mikiko Takahashi,Daiju Kitagawa
View all publications
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