JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB235513

Anti-CEP63 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal CEP63 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CEP63 aa 1-500.

View Alternative Names

Centrosomal protein of 63 kDa, Cep63, CEP63

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP63 antibody (AB235513)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP63 antibody (AB235513)

Paraffin-embedded human prostate cancer tissue stained for CEP63 using ab235513 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-CEP63 antibody (AB235513)
  • WB

Supplier Data

Western blot - Anti-CEP63 antibody (AB235513)

All lanes:

Western blot - Anti-CEP63 antibody (ab235513) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 4:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CEP63 aa 1-500. The exact immunogen used to generate this antibody is proprietary information.

Q96MT8

style
left-column

Complementary Products

Primary Antibodies

AB4448

Anti-Pericentrin antibody - Centrosome Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (AB4448)

  • 5
  • 35
Primary Antibodies

AB267377

Anti-Wilms Tumor Protein antibody [EPR23963-116]

BOND RX™ Validated|20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wilms Tumor Protein antibody [EPR23963-116] (AB267377)

  • 0
  • 0
Primary Antibodies

AB234832

Anti-ZMPSTE24 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMPSTE24 antibody (AB234832)

  • 0
  • 0
Primary Antibodies

AB154193

Anti-Von Willebrand Factor antibody [EPSISR15]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody [EPSISR15] (AB154193)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEP63 also known as Centrosomal protein 63 or CT63 is a centrosomal protein weighing approximately 79 kDa. It localizes to the centrosome an important organelle involved in cell cycle regulation. CEP63 plays an important mechanical role in centrosomal duplication. It is expressed in various tissues with higher levels noted in rapidly dividing cells particularly in testes and the brain.
Biological function summary

The functions of CEP63 extend to nucleating microtubules and maintaining centrosome cohesion. It requires association with proteins like CEP152 as part of the centrosome complex. CEP63 stabilizes the centrosome ensuring adequate centrioles duplication which is essential for cell division and maintaining genomic stability.

Pathways

CEP63 integrates into the DNA damage response and cell cycle pathways. Its role in these pathways aligns with the proteins ATR and TP53 which are important for sensing DNA damage and initiating repair or apoptosis. CEP63's involvement in these pathways supports its function in preventing genomic instability and unchecked cell division.

CEP63 has strong links to microcephaly a condition characterized by reduced brain size due to ineffective cell division in neural tissues. CEP63 mutations also connect to Seckel syndrome another disorder impacting growth and development. These disorders reveal insights into the protein's relationship with centrosome-associated proteins and DNA repair components like ATR and BRCA1 underlining its role in neurodevelopmental and proliferative pathologies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for normal spindle assembly (PubMed : 21406398, PubMed : 21983783, PubMed : 26297806, PubMed : 35793002). Plays a key role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication (PubMed : 21983783, PubMed : 26297806). Reported to be required for centrosomal recruitment of CEP152; however, this function has been questioned (PubMed : 21983783, PubMed : 26297806). Also recruits CDK1 to centrosomes (PubMed : 21406398). Plays a role in DNA damage response (PubMed : 21406398). Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and delay in mitotic progression (PubMed : 21406398). Promotes stabilization of FXR1 protein by inhibiting FXR1 ubiquitination (PubMed : 35989368).
See full target information CEP63
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 46: PubMed34296302

2021

Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1.

Applications

Unspecified application

Species

Unspecified reactive species

Chenguang Liu,Fangqin Yu,Runsheng Ma,Lele Zhang,Gongbo Du,Dongpeng Niu,Detao Yin

Frontiers in cell and developmental biology 9:686886 PubMed34150781

2021

Platelet-Derived Growth Factor-D Activates Complement System to Propagate Macrophage Polarization and Neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Xiong,Qianqian Wang,Wanhong Li,Lijuan Huang,Jianing Zhang,Juanhua Zhu,Bingbing Xie,Shasha Wang,Haiqing Kuang,Xianchai Lin,Chunsik Lee,Anil Kumar,Xuri Li
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com