Rabbit Polyclonal CEP63 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CEP63 aa 1-500.
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Publications
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- Oncology reports 46:2021Cep63 knockout inhibits the malignant phenotypes of papillary thyroid cancer cell line TPC‑1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenguang Liu,Fangqin Yu,Runsheng Ma,Lele Zhang,Gongbo Du,Dongpeng Niu,Detao YinPubMed 34296302
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CEP63 aa 1-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q96MT8
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Target data
Function
Required for normal spindle assembly (PubMed:21406398, PubMed:21983783, PubMed:26297806, PubMed:35793002). Plays a key role in mother-centriole-dependent centriole duplication; the function seems also to involve CEP152, CDK5RAP2 and WDR62 through a stepwise assembled complex at the centrosome that recruits CDK2 required for centriole duplication (PubMed:21983783, PubMed:26297806). Reported to be required for centrosomal recruitment of CEP152; however, this function has been questioned (PubMed:21983783, PubMed:26297806). Also recruits CDK1 to centrosomes (PubMed:21406398). Plays a role in DNA damage response (PubMed:21406398). Following DNA damage, such as double-strand breaks (DSBs), is removed from centrosomes; this leads to the inactivation of spindle assembly and delay in mitotic progression (PubMed:21406398). Promotes stabilization of FXR1 protein by inhibiting FXR1 ubiquitination (PubMed:35989368).
Alternative names
Centrosomal protein of 63 kDa, Cep63, CEP63
Recommended products
Rabbit Polyclonal CEP63 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CEP63 aa 1-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CEP63 aa 1-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q96MT8
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CEP63 also known as Centrosomal protein 63 or CT63 is a centrosomal protein weighing approximately 79 kDa. It localizes to the centrosome an important organelle involved in cell cycle regulation. CEP63 plays an important mechanical role in centrosomal duplication. It is expressed in various tissues with higher levels noted in rapidly dividing cells particularly in testes and the brain.
- Biological function summary
The functions of CEP63 extend to nucleating microtubules and maintaining centrosome cohesion. It requires association with proteins like CEP152 as part of the centrosome complex. CEP63 stabilizes the centrosome ensuring adequate centrioles duplication which is essential for cell division and maintaining genomic stability.
- Pathways
CEP63 integrates into the DNA damage response and cell cycle pathways. Its role in these pathways aligns with the proteins ATR and TP53 which are important for sensing DNA damage and initiating repair or apoptosis. CEP63's involvement in these pathways supports its function in preventing genomic instability and unchecked cell division.
- Associated diseases and disorders
CEP63 has strong links to microcephaly a condition characterized by reduced brain size due to ineffective cell division in neural tissues. CEP63 mutations also connect to Seckel syndrome another disorder impacting growth and development. These disorders reveal insights into the protein's relationship with centrosome-associated proteins and DNA repair components like ATR and BRCA1 underlining its role in neurodevelopmental and proliferative pathologies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CEP63 antibody (ab235513)
All lanes: Western blot - Anti-CEP63 antibody (ab235513) at 1/500 dilution
Lane 1: K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 4: Raji (human Burkitt's lymphoma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP63 antibody (ab235513)
Paraffin-embedded human prostate cancer tissue stained for CEP63 using ab235513 at 1/100 dilution in immunohistochemical analysis.
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