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AB204410

Anti-CEP89 antibody

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(4 Publications)

Rabbit Polyclonal CEP89 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CEP89 aa 650 to C-terminus.

View Alternative Names

CCDC123, CEP89, Centrosomal protein of 89 kDa, Cep89, Centrosomal protein 123, Coiled-coil domain-containing protein 123, Cep123

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP89 antibody (AB204410)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEP89 antibody (AB204410)

Immunohistochemical analysis of paraffin embedded Human lateral ventricle tissue labeling CEP89 with ab204410 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CEP89 antibody (AB204410)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CEP89 antibody (AB204410)

Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized A431 cells labeling CEP89 with ab204410 at 4 μg/mL (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CEP89 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96ST8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CEP89 also known as Centrosomal Protein 89 weighs around 89 kilodaltons. It functions as a centrosome-associated protein mainly expressed in tissues with rapidly dividing cells such as testis liver and certain brain regions. The protein localizes at the centrosome acting as a scaffold that ensures structural integrity and function during the cell cycle. Its role in centrosome dynamics highlights its importance in cellular division and proliferation processes.
Biological function summary

The protein regulates centrosome maturation and spindle assembly. It forms part of a larger molecular complex that stabilizes centrosomes during mitosis. The protein's interactions within this complex help in the recruitment of other centrosomal elements which are necessary for the proper orientation and separation of chromosomes. These interactions imply its influence in cell cycle progression and cellular integrity.

Pathways

The Centrosomal Protein 89 integrates into critical pathways in cell cycle control and mitotic spindle organization. It aligns closely with the spindle checkpoint pathway which involves proteins like Aurora A and CENP-E that ensure accurate chromosomal segregation. CEP89's influence ensures that these pathways operate smoothly contributing to chromosomal stability and preventing aneuploidy.

CEP89 has connections to disorders like primary microcephaly and some cancer types. Disruption in CEP89's function or expression can lead to abnormal centrosome numbers which affect cell division's fidelity and result in conditions like microcephaly due to improper brain development. In cancer CEP89 may intersect with proteins like p53 where aberrations contribute to unregulated cell proliferation and tumorigenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for ciliogenesis. Also plays a role in mitochondrial metabolism where it may modulate complex IV activity.
See full target information CEP89
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308820 PubMed38634253

2024

The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers.

Applications

Unspecified application

Species

Unspecified reactive species

Huicheng Chen,Zhimao Wu,Ziwei Yan,Chuan Chen,Yingying Zhang,Qiaoling Wang,Yuqing Gao,Kun Ling,Jinghua Hu,Qing Wei

Frontiers in neurology 13:1024869 PubMed36408514

2022

Structuring and validating a prognostic model for low-grade gliomas based on the genes for plasma membrane tension.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Li,Fangfang Lv,Ting Jin

Nature communications 11:2196 PubMed32366837

2020

TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Yan,Chuan Chen,Huicheng Chen,Hui Hong,Yan Huang,Kun Ling,Jinghua Hu,Qing Wei

Nature 580:106-112 PubMed32238932

2020

Centrosome anchoring regulates progenitor properties and cortical formation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Shao,Jiajun Yang,Ming He,Xiang-Yu Yu,Choong Heon Lee,Zhaohui Yang,Alexandra L Joyner,Kathryn V Anderson,Jiangyang Zhang,Meng-Fu Bryan Tsou,Hang Shi,Song-Hai Shi
View all publications
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