AB124296

Anti-Ceramide glucosyltransferase antibody

Name: Anti-Ceramide glucosyltransferase antibody (ab124296) | Abcam
Brand: Abcam Limited
SKU: AB124296
Price: 190 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal Ceramide glucosyltransferase antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

View Alternative Names

Ceramide glucosyltransferase, GLCT-1, Glucosylceramide synthase, Glycosylceramide synthase, UDP-glucose ceramide glucosyltransferase, UDP-glucose:N-acylsphingosine D-glucosyltransferase, GCS, UGCG

1 Images

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Western blot - Anti-Ceramide glucosyltransferase antibody (AB124296)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124296 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Ceramide glucosyltransferase antibody (ab124296) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Lane 4:

Spleen (Mouse) Tissue Lysate at 10 µg

Lane 5:

Spleen (Rat) Tissue Lysate at 10 µg

Lane 6:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

A375 (Human melanoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 45 kDa

Observed band size: 12 kDa,36 kDa,48 kDa,68 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in the initial step of the glucosylceramide-based glycosphingolipid/GSL synthetic pathway at the cytosolic surface of the Golgi (PubMed : 1532799, PubMed : 8643456). Catalyzes the transfer of glucose from UDP-glucose to ceramide to produce glucosylceramide/GlcCer (such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine) (PubMed : 1532799, PubMed : 8643456). GlcCer is the core component of glycosphingolipids/GSLs, amphipathic molecules consisting of a ceramide lipid moiety embedded in the outer leaflet of the membrane, linked to one of hundreds of different externally oriented oligosaccharide structures (PubMed : 8643456). Glycosphingolipids are essential components of membrane microdomains that mediate membrane trafficking and signal transduction, implicated in many fundamental cellular processes, including growth, differentiation, migration, morphogenesis, cell-to-cell and cell-to-matrix interactions (By similarity). They are required for instance in the proper development and functioning of the nervous system (By similarity). As an example of their role in signal transduction, they regulate the leptin receptor/LEPR in the leptin-mediated signaling pathway (By similarity). They also play an important role in the establishment of the skin barrier regulating keratinocyte differentiation and the proper assembly of the cornified envelope (By similarity). The biosynthesis of GSLs is also required for the proper intestinal endocytic uptake of nutritional lipids (By similarity). Catalyzes the synthesis of xylosylceramide/XylCer (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) using UDP-Xyl as xylose donor (PubMed : 33361282).

See full target information Ceramide glucosyltransferase

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3003362 PubMed40934285

2025

The mTORC2 subunit RICTOR drives breast cancer progression by promoting ganglioside biosynthesis through transcriptional and epigenetic mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Nafees Ansari,Somesh K Jha,Ali Khan,Kajal Rajput,Nishant Pandey,Dolly Jain,Rajeshwari Tripathi,Nihal Medatwal,Pankaj Sharma,Sudeshna Datta,Animesh Kar,Trishna Pani,Sk Asif Ali,Kaushavi Cholke,Kajal Rana,Valiya P Snijesh,Geetashree Mukherjee,Suryanarayana V S Deo,Soumen Basak,Ashutosh Mishra,Jyothi S Prabhu,Arnab Mukhopadhyay,Avinash Bajaj,Ujjaini Dasgupta

Science advances 9:eadf2746 PubMed37390205

2023

A localized hydrogel-mediated chemotherapy causes immunogenic cell death via activation of ceramide-mediated unfolded protein response.

Applications

Unspecified application

Species

Unspecified reactive species

Animesh Kar,Dolly Jain,Sandeep Kumar,Kajal Rajput,Sanjay Pal,Kajal Rana,Raunak Kar,Somesh K Jha,Nihal Medatwal,Prabhu Srinivas Yavvari,Nishant Pandey,Devashish Mehta,Harsh Sharma,Debanjan Bhattacharya,Manas K Pradhan,Ravi Datta Sharma,Aasheesh Srivastava,Usha Agrawal,Arnab Mukhopadhyay,Sagar Sengupta,Veena S Patil,Avinash Bajaj,Ujjaini Dasgupta

Life (Basel, Switzerland) 12: PubMed35330102

2022

Inhibition of Ceramide Glycosylation Enhances Cisplatin Sensitivity in Cholangiocarcinoma by Limiting the Activation of the ERK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Piyasiri Chueakwon,Peeranat Jatooratthawichot,Krajang Talabnin,James R Ketudat Cairns,Chutima Talabnin

The Journal of biological chemistry 293:17505-17522 PubMed30242129

2018

Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuhiro Hayashi,Yoko Nemoto-Sasaki,Naoki Matsumoto,Kotaro Hama,Takashi Tanikawa,Saori Oka,Tadaaki Saeki,Tatsuya Kumasaka,Takanori Koizumi,Seisuke Arai,Ikuo Wada,Kazuaki Yokoyama,Takayuki Sugiura,Atsushi Yamashita

Tissue engineering. Part C, Methods 21:15-22 PubMed24803151

2014

Lowered humidity produces human epidermal equivalents with enhanced barrier properties.

Applications

Unspecified application

Species

Unspecified reactive species

Richard Sun,Anna Celli,Debra Crumrine,Melanie Hupe,Lillian C Adame,Sally D Pennypacker,Kyungho Park,Yoshikazu Uchida,Kenneth R Feingold,Peter M Elias,Dusko Ilic,Theodora M Mauro

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Ceramide glucosyltransferase antibody

Western blot - Anti-Ceramide glucosyltransferase antibody (AB124296)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise