Rabbit Polyclonal CERK antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CERK aa 50-350.
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Publications
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- International journal of molecular sciences 23:2022Decreased Innate Migration of Pro-Inflammatory M1 Macrophages through the Mesothelial Membrane Is Affected by Ceramide Kinase and Ceramide 1-P.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChee Wai Ku,Joan Yang,Hong Ying Tan,Jerry Kok Yen Chan,Yie Hou LeePubMed 36555618
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CERK aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TCT0
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed:11956206, PubMed:16269826, PubMed:19168031). Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (PubMed:11956206, PubMed:16269826, PubMed:19168031). Shows a greater preference for D-erythro isomer of ceramides (PubMed:16269826). Binds phosphoinositides (PubMed:19168031).
Alternative names
KIAA1646, CERK, Ceramide kinase, hCERK, Acylsphingosine kinase, Lipid kinase 4, LK4
Recommended products
Rabbit Polyclonal CERK antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CERK aa 50-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CERK aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TCT0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CERK or ceramide kinase is an enzyme responsible for the conversion of ceramide into ceramide-1-phosphate (C1P). This enzyme has a molecular mass of approximately 66 kDa. Researchers identify CERK as 'beta CERK' due to isoform differences in some cases. CERK expression is observed in several human tissues with significant levels in the brain lungs and spleen. Under physiological conditions CERK localizes mostly to the Golgi apparatus and other intracellular membranes.
- Biological function summary
CERK plays a vital role in maintaining the balance of lipid signaling molecules within cells. It acts as an important mediator in the conversion process that produces C1P a molecule involved in signaling pathways that regulate cell survival proliferation and inflammatory responses. While not part of a larger protein complex CERK often interacts with other signaling lipids and proteins to execute its biological functions effectively.
- Pathways
CERK is an important component of the ceramide signaling pathway and the sphingolipid metabolism pathway. Within these pathways CERK works alongside sphingosine kinase and other enzymes to regulate the balance of sphingolipids which are critical for cell signaling and membrane structure. The signaling pathway involving CERK influences cellular responses by altering lipid signaling molecules affecting cellular dynamics including apoptosis and growth processes.
- Associated diseases and disorders
CERK has been linked to inflammatory diseases and cancer. Inflammatory disorders like rheumatoid arthritis exhibit altered CERK activity and expression affecting C1P signaling in the inflammatory processes. In cancer aberrant regulation of CERK can modify tumor cell survival and proliferation. This connection highlights its interaction with proteins such as sphingosine-1-phosphate in the disease mechanism indicating potential therapeutic targets for interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CERK antibody (ab155061)
7.5% SDS-PAGE.
All lanes: Western blot - Anti-CERK antibody (ab155061) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERK antibody (ab155061)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CERK with ab155061 at 1/500 dilution.
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