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AB155061

Anti-CERK antibody

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(2 Publications)

Rabbit Polyclonal CERK antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CERK aa 50-350.

View Alternative Names

KIAA1646, CERK, Ceramide kinase, hCERK, Acylsphingosine kinase, Lipid kinase 4, LK4

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERK antibody (AB155061)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERK antibody (AB155061)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling CERK with ab155061 at 1/500 dilution.

Western blot - Anti-CERK antibody (AB155061)
  • WB

Unknown

Western blot - Anti-CERK antibody (AB155061)

7.5% SDS-PAGE.

All lanes:

Western blot - Anti-CERK antibody (ab155061) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CERK aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

Q8TCT0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CERK or ceramide kinase is an enzyme responsible for the conversion of ceramide into ceramide-1-phosphate (C1P). This enzyme has a molecular mass of approximately 66 kDa. Researchers identify CERK as 'beta CERK' due to isoform differences in some cases. CERK expression is observed in several human tissues with significant levels in the brain lungs and spleen. Under physiological conditions CERK localizes mostly to the Golgi apparatus and other intracellular membranes.
Biological function summary

CERK plays a vital role in maintaining the balance of lipid signaling molecules within cells. It acts as an important mediator in the conversion process that produces C1P a molecule involved in signaling pathways that regulate cell survival proliferation and inflammatory responses. While not part of a larger protein complex CERK often interacts with other signaling lipids and proteins to execute its biological functions effectively.

Pathways

CERK is an important component of the ceramide signaling pathway and the sphingolipid metabolism pathway. Within these pathways CERK works alongside sphingosine kinase and other enzymes to regulate the balance of sphingolipids which are critical for cell signaling and membrane structure. The signaling pathway involving CERK influences cellular responses by altering lipid signaling molecules affecting cellular dynamics including apoptosis and growth processes.

CERK has been linked to inflammatory diseases and cancer. Inflammatory disorders like rheumatoid arthritis exhibit altered CERK activity and expression affecting C1P signaling in the inflammatory processes. In cancer aberrant regulation of CERK can modify tumor cell survival and proliferation. This connection highlights its interaction with proteins such as sphingosine-1-phosphate in the disease mechanism indicating potential therapeutic targets for interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed : 11956206, PubMed : 16269826, PubMed : 19168031). Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (PubMed : 11956206, PubMed : 16269826, PubMed : 19168031). Shows a greater preference for D-erythro isomer of ceramides (PubMed : 16269826). Binds phosphoinositides (PubMed : 19168031).
See full target information CERK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36555618

2022

Decreased Innate Migration of Pro-Inflammatory M1 Macrophages through the Mesothelial Membrane Is Affected by Ceramide Kinase and Ceramide 1-P.

Applications

Unspecified application

Species

Unspecified reactive species

Chee Wai Ku,Joan Yang,Hong Ying Tan,Jerry Kok Yen Chan,Yie Hou Lee

Cancer immunology research 7:1091-1105 PubMed31164356

2019

Tumor Cells Hijack Macrophage-Produced Complement C1q to Promote Tumor Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Lubka T Roumenina,Marie V Daugan,Rémi Noé,Florent Petitprez,Yann A Vano,Rafaël Sanchez-Salas,Etienne Becht,Julie Meilleroux,Bénédicte Le Clec'h,Nicolas A Giraldo,Nicolas S Merle,Cheng-Ming Sun,Virginie Verkarre,Pierre Validire,Janick Selves,Laetitia Lacroix,Olivier Delfour,Isabelle Vandenberghe,Celine Thuilliez,Sonia Keddani,Imene B Sakhi,Eric Barret,Pierre Ferré,Nathalie Corvaïa,Alexandre Passioukov,Eric Chetaille,Marina Botto,Aurélien de Reynies,Stephane Marie Oudard,Arnaud Mejean,Xavier Cathelineau,Catherine Sautès-Fridman,Wolf H Fridman
View all publications
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Product promise

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