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AB222828

Anti-CERKL antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal CERKL antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CERKL.

View Alternative Names

Ceramide kinase-like protein, CERKL

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (AB222828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (AB222828)

Paraffin-embedded human pancreatic tissue stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CERKL antibody (AB222828)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CERKL antibody (AB222828)

MCF7 (human breast adenocarcinoma cell line) cells stained for CERKL (green) using ab222828 at 1/100 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (AB222828)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (AB222828)

Paraffin-embedded human prostate cancer tissue were stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-CERKL antibody (AB222828)
  • WB

Unknown

Western blot - Anti-CERKL antibody (AB222828)

All lanes:

Western blot - Anti-CERKL antibody (ab222828) at 1/1000 dilution

All lanes:

Mouse lung tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/10000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CERKL.

Q49MI3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
>95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein CERKL also called Ceramide Kinase-Like is significant in cellular functions. CERKL has a molecular weight of approximately 70 kDa and expresses mainly in retinal tissue and other neuronal tissues. It is a member of the ceramide kinase family though it lacks measurable kinase activity. CERKL participates in cellular signaling and survival pathways suggesting an important role in maintaining neuronal health.
Biological function summary

CERKL plays a part in cellular protection against oxidative stress and apoptosis. It interacts with other proteins to form complexes that likely enhance cell survival under stressful conditions. Researchers suggest CERKL's function supports photoreceptor cells in the retinan important for light detection in vertebrate vision systems.

Pathways

CERKL contributes to the balance of apoptotic and survival signals within the PI3K/Akt pathway. Its function ensures protection against cellular damage in oxidative environments. CERKL associates with proteins such as Akt a well-known kinase regulating cell survival and growth. Its role in these pathways highlights its impact on cellular resilience.

CERKL mutations link to cases of retinitis pigmentosa and cone-rod dystrophy. These are degenerative retinal diseases leading to vision loss. The connection between CERKL and these disorders indicates its critical role in retinal health. Additionally understanding CERKL's interaction with proteins like RDH12 can offer insights into photoreceptor cell survival and possibly aid in developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
See full target information CERKL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 16:2835-2852 PubMed33061800

2020

Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Dong,Mei Feng,Hui Yang,Hengkang Liu,Hua Guo,Xianshu Gao,Yucun Liu,Rong Liu,Ning Zhang,Ruibing Chen,Ruirui Kong
View all publications
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Product promise

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