Rabbit Polyclonal CERKL antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CERKL.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CERKL. Database link Q49MI3
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.
Alternative names
Ceramide kinase-like protein, CERKL
Recommended products
Rabbit Polyclonal CERKL antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CERKL.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CERKL. Database link Q49MI3
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
>95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein CERKL also called Ceramide Kinase-Like is significant in cellular functions. CERKL has a molecular weight of approximately 70 kDa and expresses mainly in retinal tissue and other neuronal tissues. It is a member of the ceramide kinase family though it lacks measurable kinase activity. CERKL participates in cellular signaling and survival pathways suggesting an important role in maintaining neuronal health.
- Biological function summary
CERKL plays a part in cellular protection against oxidative stress and apoptosis. It interacts with other proteins to form complexes that likely enhance cell survival under stressful conditions. Researchers suggest CERKL's function supports photoreceptor cells in the retinan important for light detection in vertebrate vision systems.
- Pathways
CERKL contributes to the balance of apoptotic and survival signals within the PI3K/Akt pathway. Its function ensures protection against cellular damage in oxidative environments. CERKL associates with proteins such as Akt a well-known kinase regulating cell survival and growth. Its role in these pathways highlights its impact on cellular resilience.
- Associated diseases and disorders
CERKL mutations link to cases of retinitis pigmentosa and cone-rod dystrophy. These are degenerative retinal diseases leading to vision loss. The connection between CERKL and these disorders indicates its critical role in retinal health. Additionally understanding CERKL's interaction with proteins like RDH12 can offer insights into photoreceptor cell survival and possibly aid in developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-CERKL antibody (ab222828)
All lanes: Western blot - Anti-CERKL antibody (ab222828) at 1/1000 dilution
All lanes: Mouse lung tissue lysate
SecondaryAll lanes: Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (ab222828)
Paraffin-embedded human prostate cancer tissue were stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CERKL antibody (ab222828)
Paraffin-embedded human pancreatic tissue stained for CERKL using ab222828 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-CERKL antibody (ab222828)
MCF7 (human breast adenocarcinoma cell line) cells stained for CERKL (green) using ab222828 at 1/100 dilution in ICC/IF.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com