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AB279372

Anti-CerS2 antibody [OTI3D9]

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(2 Publications )

Mouse Monoclonal CERS2 antibody. Suitable for WB, IHC-P and reacts with Human, Transfected cell lysate - Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CERS2 aa 50-150.

View Alternative Names

LASS2, TMSG1, CERS2, Ceramide synthase 2, CerS2, LAG1 longevity assurance homolog 2, SP260, Sphingosine N-acyltransferase CERS2, Tumor metastasis-suppressor gene 1 protein, Very-long-chain ceramide synthase CERS2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CerS2 antibody [OTI3D9] (AB279372)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CerS2 antibody [OTI3D9] (AB279372)

Paraffin embedded carcinoma of human lung tissue stained for CERS2 using ab279372 at 1/1000 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3 minutes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CerS2 antibody [OTI3D9] (AB279372)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CerS2 antibody [OTI3D9] (AB279372)

Paraffin embedded Human breast tissue stained for CERS2 using ab279372 at 1/1000 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer pH8.5 at 120°C for 3 minutes.

Western blot - Anti-CerS2 antibody [OTI3D9] (AB279372)
  • WB

Supplier Data

Western blot - Anti-CerS2 antibody [OTI3D9] (AB279372)

The blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody for loading control purposes.

Lanes 1 - 2:

Western blot - Anti-CerS2 antibody [OTI3D9] (ab279372) at 1/500 dilution

Lanes 3 - 4:

anti-HSP90AB1 at 1/500 dilution

Lanes 1 and 3:

Wild-type HEK-293T cells at 10 µg

Lanes 2 and 4:

CERS2-Knockout HEK-293T cells at 10 µg

Predicted band size: 45 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI3D9

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CERS2 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q96G23

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Secondary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ceramide synthase that catalyzes the transfer of the acyl chain from acyl-CoA to a sphingoid base, with high selectivity toward very-long-chain fatty acyl-CoA (chain length C22-C27) (PubMed : 17977534, PubMed : 18165233, PubMed : 18541923, PubMed : 19728861, PubMed : 20937905, PubMed : 22144673, PubMed : 22661289, PubMed : 26887952, PubMed : 29632068). N-acylates sphinganine and sphingosine bases to form dihydroceramides and ceramides in de novo synthesis and salvage pathways, respectively (By similarity) (PubMed : 17977534, PubMed : 18165233, PubMed : 18541923, PubMed : 19728861, PubMed : 20937905, PubMed : 22144673, PubMed : 22661289, PubMed : 26887952, PubMed : 29632068). Plays a non-redundant role in the synthesis of ceramides with very-long-chain fatty acids in kidney, liver and brain. Regulates the abundance of myelin-specific sphingolipids galactosylceramide and sulfatide that affects myelin sheath architecture and motor neuron functions (By similarity).
See full target information CERS2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of ovarian research 17:61 PubMed38486335

2024

Unraveling the molecular mechanisms of lymph node metastasis in ovarian cancer: focus on MEOX1.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Li,Yihua Sun,Xiuling Zhi,Yating Sun,Zulimire Abudousalamu,Qianhan Lin,Bin Li,Liangqing Yao,Mo Chen

BMC medicine 22:19 PubMed38191448

2024

LASS2 enhances chemosensitivity to cisplatin by inhibiting PP2A-mediated β-catenin dephosphorylation in a subset of stem-like bladder cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjin Shi,Zhiyong Tan,Bowen Duan,Chunming Guo,Chong Li,Ting Luan,Ning Li,Yinglong Huang,Shi Chen,Jixian Gao,Wei Feng,Haole Xu,Jiansong Wang,Shi Fu,Haifeng Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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