Rabbit Polyclonal CERT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human CERT1 aa 300-350.
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- The Journal of biological chemistry 298:1025892022Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomasz H Benedyk,Viv Connor,Eve R Caroe,Maria Shamin,Dmitri I Svergun,Janet E Deane,Cy M Jeffries,Colin M Crump,Stephen C GrahamPubMed 36243114
- International journal of molecular sciences 23:2022Involvement of a Cluster of Basic Amino Acids in Phosphorylation-Dependent Functional Repression of the Ceramide Transport Protein CERT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsako Goto,Daichi Egawa,Nario Tomishige,Toshiyuki Yamaji,Kentaro Shimasaki,Keigo Kumagai,Kentaro HanadaPubMed 35955719
- Cell chemical biology 29:1419-1433.e52022A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKamalakannan Vijayan,Nadia Arang,Ling Wei,Robert Morrison,Rechel Geiger,K Rachael Parks,Adam J Lewis,Fred D Mast,Alyse N Douglass,Heather S Kain,John D Aitchison,Jarrod S Johnson,Alan Aderem,Alexis KaushanskyPubMed 35738280
- iScience 25:1046242022Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsako Goto,Shota Sakai,Aya Mizuike,Toshiyuki Yamaji,Kentaro HanadaPubMed 35800758
- Developmental cell 57:974-994.e82022VAP-A and its binding partner CERT drive biogenesis of RNA-containing extracellular vesicles at ER membrane contact sites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBahnisikha Barman,Bong Hwan Sung,Evan Krystofiak,Jie Ping,Marisol Ramirez,Bryan Millis,Ryan Allen,Nripesh Prasad,Sergei Chetyrkin,M Wade Calcutt,Kasey Vickers,James G Patton,Qi Liu,Alissa M WeaverPubMed 35421371
- International journal of molecular sciences 23:2022Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKentaro Shimasaki,Keigo Kumagai,Shota Sakai,Toshiyuki Yamaji,Kentaro HanadaPubMed 35409383
- The Journal of biological chemistry 297:1013382021Intellectual-disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNorito Tamura,Shota Sakai,Loreto Martorell,Roser Colomé,Aya Mizuike,Asako Goto,Juan Darío Ortigoza-Escobar,Kentaro HanadaPubMed 34688657
- PLoS pathogens 17:e10098242021pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomasz H Benedyk,Julia Muenzner,Viv Connor,Yue Han,Katherine Brown,Kaveesha J Wijesinghe,Yunhui Zhuang,Susanna Colaco,Guido A Stoll,Owen S Tutt,Stanislava Svobodova,Dmitri I Svergun,Neil A Bryant,Janet E Deane,Andrew E Firth,Cy M Jeffries,Colin M Crump,Stephen C GrahamPubMed 34398933
- PloS one 15:e02439802020Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroaki Murakami et. al.PubMed 33347465
- Nature communications 11:61732020Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRalph Götz,Tobias C Kunz,Julian Fink,Franziska Solger,Jan Schlegel,Jürgen Seibel,Vera Kozjak-Pavlovic,Thomas Rudel,Markus SauerPubMed 33268771
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CERT1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5P4
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Turkey | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Guinea pig, Chimpanzee | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2500.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Guinea pig, Chimpanzee | Dilution info - | Notes - |
Associated Products
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Target data
Function
Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.
Alternative names
CERT, COL4A3BP, STARD11, CERT1, Ceramide transfer protein, hCERT, Collagen type IV alpha-3-binding protein, Goodpasture antigen-binding protein, START domain-containing protein 11, StAR-related lipid transfer protein 11, GPBP, StARD11
Recommended products
Rabbit Polyclonal CERT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human CERT1 aa 300-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CERT1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y5P4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CERT also known as CERT protein or COL4A3BP functions in the intracellular transport of ceramide from the endoplasmic reticulum to the Golgi apparatus. CERT consists of a PH domain a middle domain and a START domain comprising a mass of approximately 68 kDa. It is expressed in various human tissues with notable expression in the brain and liver. CERT facilitates the synthesis of sphingomyelin an essential component of cellular membranes.
- Biological function summary
CERT plays a central role in lipid metabolism and is part of the ceramide transfer complex. This complex regulates the balance of ceramide and sphingomyelin as these lipids influence cell signaling and apoptosis. The effective transfer of ceramide by CERT ensures proper cell function and integrity. Aberrations in CERT activity can disrupt lipid homeostasis impacting cell survival and signaling pathways.
- Pathways
CERT is integrated into the sphingolipid and lipid signaling pathways. It interacts with proteins like protein kinase D which regulates its phosphorylation status and as a result its activity. In these pathways CERT's role is important for maintaining lipid composition within cell membranes ensuring the proper functioning of signaling processes that depend on lipid-mediated communication.
- Associated diseases and disorders
Disrupted CERT function associates with conditions like cancer and neurodegenerative disorders. In cancer altered ceramide levels due to CERT dysfunction can influence cell growth and survival contributing to tumor progression. For neurodegenerative disorders CERT's interaction with proteins like protein kinase C highlights its significance in maintaining neuronal cell stability and signaling disruption of which may relate to disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-CERT antibody (ab72536)
All lanes: Western blot - Anti-CERT antibody (ab72536) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Predicted band size: 71 kDa
Observed band size: 100 kDa, 28 kDa, 60 kDa, 70 kDa, 71 kDa
Immunoprecipitation - Anti-CERT antibody (ab72536)
Detection of Human CERT by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72536 at 3 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot detection.
All lanes: Immunoprecipitation - Anti-CERT antibody (ab72536)
Predicted band size: 71 kDa
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