AB72536

Anti-CERT antibody

Name: Anti-CERT antibody (ab72536) | Abcam
Brand: Abcam Limited
SKU: AB72536
Price: 545 USD
Availability: InStock

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(14 Publications)

Rabbit Polyclonal CERT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human CERT1 aa 300-350.

View Alternative Names

CERT, COL4A3BP, STARD11, CERT1, Ceramide transfer protein, hCERT, Collagen type IV alpha-3-binding protein, Goodpasture antigen-binding protein, START domain-containing protein 11, StAR-related lipid transfer protein 11, GPBP, StARD11

2 Images

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Immunoprecipitation - Anti-CERT antibody (AB72536)

Detection of Human CERT by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72536 at 3 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot detection.

All lanes:

Immunoprecipitation - Anti-CERT antibody (ab72536)

Predicted band size: 71 kDa

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Western blot - Anti-CERT antibody (AB72536)

All lanes:

Western blot - Anti-CERT antibody (ab72536) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 71 kDa

Observed band size: 100 kDa,28 kDa,60 kDa,70 kDa,71 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CERT1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5P4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/10000", "WB-species-notes": "<p></p>" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CERT also known as CERT protein or COL4A3BP functions in the intracellular transport of ceramide from the endoplasmic reticulum to the Golgi apparatus. CERT consists of a PH domain a middle domain and a START domain comprising a mass of approximately 68 kDa. It is expressed in various human tissues with notable expression in the brain and liver. CERT facilitates the synthesis of sphingomyelin an essential component of cellular membranes.

Biological function summary

CERT plays a central role in lipid metabolism and is part of the ceramide transfer complex. This complex regulates the balance of ceramide and sphingomyelin as these lipids influence cell signaling and apoptosis. The effective transfer of ceramide by CERT ensures proper cell function and integrity. Aberrations in CERT activity can disrupt lipid homeostasis impacting cell survival and signaling pathways.

Pathways

CERT is integrated into the sphingolipid and lipid signaling pathways. It interacts with proteins like protein kinase D which regulates its phosphorylation status and as a result its activity. In these pathways CERT's role is important for maintaining lipid composition within cell membranes ensuring the proper functioning of signaling processes that depend on lipid-mediated communication.

Disrupted CERT function associates with conditions like cancer and neurodegenerative disorders. In cancer altered ceramide levels due to CERT dysfunction can influence cell growth and survival contributing to tumor progression. For neurodegenerative disorders CERT's interaction with proteins like protein kinase C highlights its significance in maintaining neuronal cell stability and signaling disruption of which may relate to disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Shelters ceramides and diacylglycerol lipids inside its START domain and mediates the intracellular trafficking of ceramides and diacylglycerol lipids in a non-vesicular manner.

See full target information CERT1

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 298:102589 PubMed36243114

2022

Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the interorganelle transport protein CERT.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz H Benedyk,Viv Connor,Eve R Caroe,Maria Shamin,Dmitri I Svergun,Janet E Deane,Cy M Jeffries,Colin M Crump,Stephen C Graham

International journal of molecular sciences 23: PubMed35955719

2022

Involvement of a Cluster of Basic Amino Acids in Phosphorylation-Dependent Functional Repression of the Ceramide Transport Protein CERT.

Applications

Unspecified application

Species

Unspecified reactive species

Asako Goto,Daichi Egawa,Nario Tomishige,Toshiyuki Yamaji,Kentaro Shimasaki,Keigo Kumagai,Kentaro Hanada

Cell chemical biology 29:1419-1433.e5 PubMed35738280

2022

A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection.

Applications

Unspecified application

Species

Unspecified reactive species

Kamalakannan Vijayan,Nadia Arang,Ling Wei,Robert Morrison,Rechel Geiger,K Rachael Parks,Adam J Lewis,Fred D Mast,Alyse N Douglass,Heather S Kain,John D Aitchison,Jarrod S Johnson,Alan Aderem,Alexis Kaushansky

iScience 25:104624 PubMed35800758

2022

Compartmentalization of casein kinase 1 γ CSNK1G controls the intracellular trafficking of ceramide.

Applications

Unspecified application

Species

Unspecified reactive species

Asako Goto,Shota Sakai,Aya Mizuike,Toshiyuki Yamaji,Kentaro Hanada

Developmental cell 57:974-994.e8 PubMed35421371

2022

VAP-A and its binding partner CERT drive biogenesis of RNA-containing extracellular vesicles at ER membrane contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Bahnisikha Barman,Bong Hwan Sung,Evan Krystofiak,Jie Ping,Marisol Ramirez,Bryan Millis,Ryan Allen,Nripesh Prasad,Sergei Chetyrkin,M Wade Calcutt,Kasey Vickers,James G Patton,Qi Liu,Alissa M Weaver

International journal of molecular sciences 23: PubMed35409383

2022

Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Kentaro Shimasaki,Keigo Kumagai,Shota Sakai,Toshiyuki Yamaji,Kentaro Hanada

The Journal of biological chemistry 297:101338 PubMed34688657

2021

Intellectual-disability-associated mutations in the ceramide transport protein gene CERT1 lead to aberrant function and subcellular distribution.

Applications

Unspecified application

Species

Unspecified reactive species

Norito Tamura,Shota Sakai,Loreto Martorell,Roser Colomé,Aya Mizuike,Asako Goto,Juan Darío Ortigoza-Escobar,Kentaro Hanada

PLoS pathogens 17:e1009824 PubMed34398933

2021

pUL21 is a viral phosphatase adaptor that promotes herpes simplex virus replication and spread.

Applications

Unspecified application

Species

Unspecified reactive species

Tomasz H Benedyk,Julia Muenzner,Viv Connor,Yue Han,Katherine Brown,Kaveesha J Wijesinghe,Yunhui Zhuang,Susanna Colaco,Guido A Stoll,Owen S Tutt,Stanislava Svobodova,Dmitri I Svergun,Neil A Bryant,Janet E Deane,Andrew E Firth,Cy M Jeffries,Colin M Crump,Stephen C Graham

PloS one 15:e0243980 PubMed33347465

2020

Intellectual disability-associated gain-of-function mutations in CERT1 that encodes the ceramide transport protein CERT.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Murakami,Norito Tamura,Yumi Enomoto,Kentaro Shimasaki,Kenji Kurosawa,Kentaro Hanada

Nature communications 11:6173 PubMed33268771

2020

Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Ralph Götz,Tobias C Kunz,Julian Fink,Franziska Solger,Jan Schlegel,Jürgen Seibel,Vera Kozjak-Pavlovic,Thomas Rudel,Markus Sauer

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CERT antibody

Immunoprecipitation - Anti-CERT antibody (AB72536)

Western blot - Anti-CERT antibody (AB72536)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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