Rabbit Recombinant Monoclonal Ceruloplasmin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IP | IHC-P | |
---|---|---|---|
Human | Tested | Not recommended | Not recommended |
Mouse | Tested | Not recommended | Not recommended |
Rat | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Select an associated product type
Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane (PubMed:16150804). Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin (PubMed:14623105, PubMed:4643313, PubMed:5912351). Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1 (By similarity). Has glutathione peroxidase-like activity, can remove both hydrogen peroxide and lipid hydroperoxide in the presence of thiols (PubMed:10481051). Also shows NO-oxidase and NO2 synthase activities that determine endocrine NO homeostasis (PubMed:16906150).
Ceruloplasmin, Cuproxidase ceruloplasmin, Ferroxidase ceruloplasmin, Glutathione peroxidase ceruloplasmin, Glutathione-dependent peroxiredoxin ceruloplasmin, CP
Rabbit Recombinant Monoclonal Ceruloplasmin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR28432-1
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Deglycosylation induced a shift in the observed molecular weight from 150 kDa and 135 kDa to 135 kDa and 115 kDa.
All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668) at 1/1000 dilution
Lane 1: Untreated human plasma at 20 µg
Lane 2: Human plasma treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa, 135 kDa, 115 kDa
Exposure time: 3.25s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: kidney (PMID: 8690795).
The identity of the band around 37 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668) at 1/1000 dilution
Lane 1: Mouse serum at 20 µg
Lane 2: Mouse kidney tissue lysate at 20 µg
Lane 3: Rat serum at 20 µg
Lane 4: Human serum at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa
Exposure time: 3.25s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: skeletal muscle(PMID: 8690795)
The band (130 kDa) beneath the target band (150 kDa) may be additional transcript products (PMID: 36595688, 2355023, 19690651).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668) at 1/1000 dilution
Lane 1: Human plasma at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa
Exposure time: 3.25s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: small intestine, skeletal muscle(PMID: 8690795)
The identity of the band around 37 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse plasma at 20 µg
Lane 3: Mouse small intestine tissue lysate at 20 µg
Lane 4: Mouse skeletal muscle tissue lysate at 20 µg
Lane 5: Rat liver tissue lysate at 20 µg
Lane 6: Rat plasma at 20 µg
Lane 7: Rat skeletal muscle tissue lysate at 20 µg
Lane 8: Rat small intestine tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 150 kDa
Exposure time: 180s
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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