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Rabbit Recombinant Monoclonal Ceruloplasmin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (AB314668), expandable thumbnail
  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (AB314668), expandable thumbnail
  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (AB314668), expandable thumbnail
  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (AB314668), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-P
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane (PubMed:16150804). Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin (PubMed:14623105, PubMed:4643313, PubMed:5912351). Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1 (By similarity). Has glutathione peroxidase-like activity, can remove both hydrogen peroxide and lipid hydroperoxide in the presence of thiols (PubMed:10481051). Also shows NO-oxidase and NO2 synthase activities that determine endocrine NO homeostasis (PubMed:16906150).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Ceruloplasmin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28432-1

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668), expandable thumbnail

    Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Deglycosylation induced a shift in the observed molecular weight from 150 kDa and 135 kDa to 135 kDa and 115 kDa.

    All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668) at 1/1000 dilution

    Lane 1: Untreated human plasma at 20 µg

    Lane 2: Human plasma treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa, 135 kDa, 115 kDa

    Exposure time: 3.25s

  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668), expandable thumbnail

    Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: kidney (PMID: 8690795).
    The identity of the band around 37 kDa is unknown.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668) at 1/1000 dilution

    Lane 1: Mouse serum at 20 µg

    Lane 2: Mouse kidney tissue lysate at 20 µg

    Lane 3: Rat serum at 20 µg

    Lane 4: Human serum at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa

    Exposure time: 3.25s

  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668), expandable thumbnail

    Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: skeletal muscle(PMID: 8690795)
    The band (130 kDa) beneath the target band (150 kDa) may be additional transcript products (PMID: 36595688, 2355023, 19690651).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668) at 1/1000 dilution

    Lane 1: Human plasma at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa

    Exposure time: 3.25s

  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668), expandable thumbnail

    Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: small intestine, skeletal muscle(PMID: 8690795)
    The identity of the band around 37 kDa is unknown.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (ab314668) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: Mouse plasma at 20 µg

    Lane 3: Mouse small intestine tissue lysate at 20 µg

    Lane 4: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 5: Rat liver tissue lysate at 20 µg

    Lane 6: Rat plasma at 20 µg

    Lane 7: Rat skeletal muscle tissue lysate at 20 µg

    Lane 8: Rat small intestine tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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