Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (AB314669)

This data was developed using ab314668, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Deglycosylation induced a shift in the observed molecular weight from 150 kDa and 135 kDa to 135 kDa and 115 kDa.

All lanes:

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (<a href='/en-us/products/primary-antibodies/ceruloplasmin-antibody-epr28432-1-ab314668'>ab314668</a>) at 1/1000 dilution

Lane 1:

Untreated human plasma at 20 µg

Lane 2:

Human plasma treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa,135 kDa,115 kDa

false

Exposure time: 3.25s

• WB

Supplier Data

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (AB314669)

This data was developed using ab314668, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : skeletal muscle(PMID : 8690795) The band (130 kDa) beneath the target band (150 kDa) may be additional transcript products (PMID : 36595688, 2355023, 19690651). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (<a href='/en-us/products/primary-antibodies/ceruloplasmin-antibody-epr28432-1-ab314668'>ab314668</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa

false

Exposure time: 3.25s

• WB

Supplier Data

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (AB314669)

This data was developed using ab314668, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : kidney (PMID : 8690795). The identity of the band around 37 kDa is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (<a href='/en-us/products/primary-antibodies/ceruloplasmin-antibody-epr28432-1-ab314668'>ab314668</a>) at 1/1000 dilution

Lane 1:

Mouse serum at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Rat serum at 20 µg

Lane 4:

Human serum at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa

false

Exposure time: 3.25s

• WB

Supplier Data

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (AB314669)

This data was developed using ab314668, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : small intestine, skeletal muscle(PMID : 8690795) The identity of the band around 37 kDa is unknown. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (<a href='/en-us/products/primary-antibodies/ceruloplasmin-antibody-epr28432-1-ab314668'>ab314668</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse plasma at 20 µg

Lane 3:

Mouse small intestine tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 5:

Rat liver tissue lysate at 20 µg

Lane 6:

Rat plasma at 20 µg

Lane 7:

Rat skeletal muscle tissue lysate at 20 µg

Lane 8:

Rat small intestine tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa

false

Exposure time: 180s