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Rabbit Recombinant Monoclonal Ceruloplasmin antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (AB314669), expandable thumbnail
  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (AB314669), expandable thumbnail
  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (AB314669), expandable thumbnail
  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (AB314669), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-P
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Multifunctional blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane (PubMed:16150804). Copper ions provide a large number of enzymatic activites. Oxidizes highly toxic ferrous ions to the ferric state for further incorporation onto apo-transferrins, catalyzes Cu(+) oxidation and promotes the oxidation of biogenic amines such as norepinephrin and serotonin (PubMed:14623105, PubMed:4643313, PubMed:5912351). Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1 (By similarity). Has glutathione peroxidase-like activity, can remove both hydrogen peroxide and lipid hydroperoxide in the presence of thiols (PubMed:10481051). Also shows NO-oxidase and NO2 synthase activities that determine endocrine NO homeostasis (PubMed:16906150).

Alternative names

Ceruloplasmin, Cuproxidase ceruloplasmin, Ferroxidase ceruloplasmin, Glutathione peroxidase ceruloplasmin, Glutathione-dependent peroxiredoxin ceruloplasmin, CP

Recommended products

Rabbit Recombinant Monoclonal Ceruloplasmin antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28432-1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab314669 is the carrier-free version of Anti-Ceruloplasmin antibody [EPR28432-1] ab314668.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (ab314669), expandable thumbnail

    Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (ab314669)

    This data was developed using Anti-Ceruloplasmin antibody [EPR28432-1] ab314668, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Deglycosylation induced a shift in the observed molecular weight from 150 kDa and 135 kDa to 135 kDa and 115 kDa.

    All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (Anti-Ceruloplasmin antibody [EPR28432-1] ab314668) at 1/1000 dilution

    Lane 1: Untreated human plasma at 20 µg

    Lane 2: Human plasma treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa, 135 kDa, 115 kDa

    Exposure time: 3.25s

  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (ab314669), expandable thumbnail

    Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (ab314669)

    This data was developed using Anti-Ceruloplasmin antibody [EPR28432-1] ab314668, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: kidney (PMID: 8690795).
    The identity of the band around 37 kDa is unknown.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (Anti-Ceruloplasmin antibody [EPR28432-1] ab314668) at 1/1000 dilution

    Lane 1: Mouse serum at 20 µg

    Lane 2: Mouse kidney tissue lysate at 20 µg

    Lane 3: Rat serum at 20 µg

    Lane 4: Human serum at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa

    Exposure time: 3.25s

  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (ab314669), expandable thumbnail

    Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (ab314669)

    This data was developed using Anti-Ceruloplasmin antibody [EPR28432-1] ab314668, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: skeletal muscle(PMID: 8690795)
    The band (130 kDa) beneath the target band (150 kDa) may be additional transcript products (PMID: 36595688, 2355023, 19690651).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (Anti-Ceruloplasmin antibody [EPR28432-1] ab314668) at 1/1000 dilution

    Lane 1: Human plasma at 20 µg

    Lane 2: Human skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa

    Exposure time: 3.25s

  • Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (ab314669), expandable thumbnail

    Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] - BSA and Azide free (ab314669)

    This data was developed using Anti-Ceruloplasmin antibody [EPR28432-1] ab314668, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: small intestine, skeletal muscle(PMID: 8690795)
    The identity of the band around 37 kDa is unknown.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Ceruloplasmin antibody [EPR28432-1] (Anti-Ceruloplasmin antibody [EPR28432-1] ab314668) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: Mouse plasma at 20 µg

    Lane 3: Mouse small intestine tissue lysate at 20 µg

    Lane 4: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 5: Rat liver tissue lysate at 20 µg

    Lane 6: Rat plasma at 20 µg

    Lane 7: Rat skeletal muscle tissue lysate at 20 µg

    Lane 8: Rat small intestine tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 150 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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