JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB137606

Anti-CES2 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal EST2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CES2 aa 50 to C-terminus.

View Alternative Names

ICE, CES2, Cocaine esterase, Carboxylesterase 2, Methylumbelliferyl-acetate deacetylase 2, CE-2, hCE-2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody (AB137606)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody (AB137606)

Immunohistochemical analysis of paraffin-embedded H1299 xenograft labelling CES2 with ab137606 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CES2 antibody (AB137606)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CES2 antibody (AB137606)

Immunofluorescent analysis of HepG2 cells fixed in iced-cold MeOH for 5 minutes, labelling CES2 with ab137606 at 1/500 dilution (Green).
Blue : Hoechst 33342 staining.

Western blot - Anti-CES2 antibody (AB137606)
  • WB

Unknown

Western blot - Anti-CES2 antibody (AB137606)

7.5% SDS PAGE

All lanes:

Western blot - Anti-CES2 antibody (ab137606) at 1/5000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg/mL

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CES2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00748

style
left-column

Alternative Products

Primary Antibodies

AB126970

Anti-CES2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody (AB126970)

  • 0
  • 0
Primary Antibodies

AB88439

Anti-CES2 antibody

Western blot - Anti-CES2 antibody (AB88439)

  • 0
  • 0
Primary Antibodies

AB215042

Anti-CES2 antibody

Western blot - Anti-CES2 antibody (AB215042)

  • 0
  • 0
Primary Antibodies

AB184957

Anti-CES2 antibody [EPR14856]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CES2 antibody [EPR14856] (AB184957)

  • 1
  • 1

Complementary Products

Assay Kits

AB313529

Human CES2 ELISA Kit

Sandwich ELISA - Human CES2 ELISA Kit (AB313529)

  • 0
  • 0
Primary Antibodies

AB51067

Anti-Met (c-Met) antibody [EP1454Y] - N-terminal

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Met (c-Met) antibody [EP1454Y] - N-terminal (AB51067)

  • 5
  • 13
Primary Antibodies

AB211017

Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR20374] (AB211017)

  • 5
  • 1
Primary Antibodies

AB32152

Anti-VEGF Receptor 1 antibody [Y103]

RabMAb|Recombinant|20ul selling size

Immunoprecipitation - Anti-VEGF Receptor 1 antibody [Y103] (AB32152)

  • 5
  • 21
Primary Antibodies

AB32063

Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|Advanced Validation|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (AB32063)

  • 4
  • 21
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Carboxylesterase 2 (CES2) also known as intestinal carboxylesterase is an enzyme with a molecular mass of approximately 60 kDa. CES2 hydrolyzes esters and amides affecting metabolism of drugs and xenobiotics. It is found mainly in the liver and small intestine with lesser expression in the colon kidney and brain. This distribution enables CES2 to play a role in both intestinal and systemic drug metabolism.
Biological function summary

CES2 acts in the hydrolysis process converting prodrugs into active compounds. It functions independently and is not typically part of a larger complex. This enzyme demonstrates broad substrate specificity impacting the metabolic activation of several therapeutic agents. Its activity affects the bioavailability and activation of medicines such as irinotecan and capecitabine which are used in cancer treatments.

Pathways

CES2 takes part in the drug metabolism pathway involving cytochrome P450 enzymes. It interacts with CYP3A4 one of the key enzymes in this system and facilitates the conversion of chemical compounds into pharmacologically active or inactive metabolites. CES2 also participates in lipid metabolism affecting the ester and triglyceride hydrolysis alongside lipases and other related enzymes.

CES2 has implications in colorectal cancer and liver disease conditions where altered drug metabolism influences disease progression and treatment efficacy. It shows associations with irinotecan activation a drug used for treating colorectal cancer and links with UDP-glucuronosyltransferases (UGTs) that also engage in drug metabolism. Understanding CES2's function aids in predicting patient responses to drug therapies and refining treatment strategies for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed : 9169443). Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine (PubMed : 9169443). Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol (PubMed : 28677105). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed : 21049984).
See full target information CES2
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 292:146-160 PubMed27881673

2016

Sortilin 1 Modulates Hepatic Cholesterol Lipotoxicity in Mice via Functional Interaction with Liver Carboxylesterase 1.

Applications

Unspecified application

Species

Unspecified reactive species

Jibiao Li,Yifeng Wang,David J Matye,Hemantkumar Chavan,Partha Krishnamurthy,Feng Li,Tiangang Li

Drug metabolism and disposition: the biological fate of chemicals 44:1313-8 PubMed27130352

2016

Biotransformation Capacity of Carboxylesterase in Skin and Keratinocytes for the Penta-Ethyl Ester Prodrug of DTPA.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Fu,Matthew Sadgrove,Lesley Marson,Michael Jay
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com