AB276054

Anti-CES3/TGH antibody [372] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal EST3 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CES3 aa 1 to C-terminus.

View Alternative Names

UNQ869/PRO1887, CES3, Carboxylesterase 3, Liver carboxylesterase 31 homolog

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Western blot - Anti-CES3/TGH antibody [372] - BSA and Azide free (AB276054)

Developed using the Odyssey technique.
Performed under reducing conditions.

All lanes:

Western blot - Anti-CES3/TGH antibody [372] - BSA and Azide free (ab276054) at 1/500 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 62 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

372

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human CES3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6UWW8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CES3 also known as carboxylesterase 3 or TG hydrolase (TGH) is an enzyme with a molecular mass of approximately 60 kDa. This protein functions by breaking down ester bonds effectively hydrolyzing lipids into free fatty acids and glycerol. CES3 is mainly expressed in the liver but you can also find it in other tissues including the intestine and adipose tissue. It can act on a broad range of substrates due to its versatile enzymatic activity.

Biological function summary

CES3 plays an integral role in lipid metabolism. It is not typically a part of multi-protein complexes but works independently to hydrolyze triglycerides. By doing so CES3 helps release stored energy in the form of free fatty acids which are important for various metabolic processes. This enzyme regulates energy balance and lipid storage impacting lipid signaling pathways within cells.

Pathways

CES3 participates actively in lipid metabolism and energy homeostasis pathways. It ties closely to the lipolysis pathway where it works along with proteins like hormone-sensitive lipase to mobilize stored fats for energy. Moreover CES3 interacts with lipid biosynthesis pathways linking lipid storage with breakdown and playing a significant role in maintaining lipid equilibrium in the body.

Alterations in CES3 function relate to conditions such as obesity and non-alcoholic fatty liver disease (NAFLD). Reduced or excessive CES3 activity might affect lipid metabolism thereby contributing to abnormal fat accumulation. CES3 is connected to hepatic lipase which also influences lipid processing in the liver demonstrating the enzyme’s potential impact on liver health and systemic lipid balance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows low catalytic efficiency for hydrolysis of CPT-11 (7-ethyl-10-[4-(1-piperidino)-1-piperidino]-carbonyloxycamptothecin), a prodrug for camptothecin used in cancer therapeutics.

See full target information CES3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CES3/TGH antibody [372] - BSA and Azide free

Western blot - Anti-CES3/TGH antibody [372] - BSA and Azide free (AB276054)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise