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AB249291

Anti-CETP antibody [EPR13] - BSA and Azide free

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Rabbit Recombinant Monoclonal CETP antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

Cholesteryl ester transfer protein, Lipid transfer protein I, CETP

2 Images
Immunoprecipitation - Anti-CETP antibody [EPR13] - BSA and Azide free (AB249291)
  • IP

Unknown

Immunoprecipitation - Anti-CETP antibody [EPR13] - BSA and Azide free (AB249291)

This data was developed using ab157183, the same antibody clone in a different buffer formulation.

Detection of CETP by Western Blot of Immunprecipitate. Human plasma lysates immunoprecipitated using ab157183 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-CETP antibody [EPR13] (<a href='/en-us/products/primary-antibodies/cetp-antibody-epr13-ab157183'>ab157183</a>) at 1/1000 dilution

All lanes:

immunoprecipitation pellets from Human plasma at 10 µg

Predicted band size: 181 kDa,33 kDa,38 kDa,55 kDa

Observed band size: 50 kDa

false

Western blot - Anti-CETP antibody [EPR13] - BSA and Azide free (AB249291)
  • WB

Unknown

Western blot - Anti-CETP antibody [EPR13] - BSA and Azide free (AB249291)

This data was developed using ab157183, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CETP antibody [EPR13] (<a href='/en-us/products/primary-antibodies/cetp-antibody-epr13-ab157183'>ab157183</a>) at 1/1000 dilution

All lanes:

Human plasma lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab249291 is the carrier-free version of ab157183.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CETP also known as Cholesteryl Ester Transfer Protein is a plasma glycoprotein with a molecular mass of approximately 74 kDa. It is primarily expressed in the liver and circulates in the bloodstream. CETP mediates the transfer of cholesteryl esters and triglycerides between high-density lipoproteins (HDL) and low-density lipoproteins (LDL) influencing lipid balance in human plasma. This protein is essential for regulating the distribution and composition of HDL and LDL particles impacting lipid homeostasis.
Biological function summary

CETP plays a role in lipid metabolism by facilitating the redistribution of lipids among various lipoprotein particles. CETP does not function as part of a larger molecular complex but actively interacts with lipoproteins in a reversible manner. Its activity affects the levels of HDL cholesterol often referred to as "good cholesterol" and contributes to maintaining lipid levels within physiological limits. By modulating the lipid exchanges CETP influences atherosclerosis progression and cardiovascular health.

Pathways

CETP interacts significantly with lipoprotein metabolism and reverse cholesterol transport pathways. It works alongside key proteins such as apolipoprotein A-I and apolipoprotein B which are present in HDL and LDL particles respectively. CETP activity in these pathways modulates the cholesterol efflux capability of HDL affecting lipid clearance from tissues back to the liver. These interactions ensure the maintenance of cholesterol equilibrium and are vital in lipid recycling processes.

CETP is closely linked to cardiovascular diseases and atherosclerosis. Elevated CETP activity correlates with low HDL cholesterol levels and higher risk of coronary artery disease. CETP inhibitors have been researched for their potential to increase HDL levels and reduce cardiovascular risk. Furthermore CETP activity influences the pathology of metabolic syndrome where lipid imbalances exacerbate the condition. Proteins related to CETP in these contexts include HDL and LDL which are altered in their distributions by CETP's activity impacting disease progression and management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the transfer of neutral lipids, including cholesteryl ester and triglyceride, among lipoprotein particles. Allows the net movement of cholesteryl ester from high density lipoproteins/HDL to triglyceride-rich very low density lipoproteins/VLDL, and the equimolar transport of triglyceride from VLDL to HDL (PubMed : 24293641, PubMed : 3281933, PubMed : 3600759). Regulates the reverse cholesterol transport, by which excess cholesterol is removed from peripheral tissues and returned to the liver for elimination (PubMed : 17237796).
See full target information CETP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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