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Mouse Monoclonal CD9 antibody - conjugated to CF®405M. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Tissue preparation containing CD9 protein.

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Images

Flow Cytometry - CF405M Anti-CD9 antibody [VJ1/20] (AB123624), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Conjugation
CF®405M
Storage buffer

pH: 7.2
Preservative: 0.09% Sodium azide

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Tissue preparation containing CD9 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P21926

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
5 µL for 106 Cells
Notes

ab126036 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Associated Products

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1 product for Alternative Version

Target data

Function

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:14575715, PubMed:18541721, PubMed:8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed:12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed:18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed:7511626, PubMed:8478605).

Alternative names

CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29

Recommended products

Mouse Monoclonal CD9 antibody - conjugated to CF®405M. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Tissue preparation containing CD9 protein.

Key facts

Isotype
IgG2a
Conjugation
CF®405M
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Tissue preparation containing CD9 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P21926
Clone number
VJ1/20
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.

Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

Associated diseases and disorders

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.

Product promise

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1 product image

  • Flow Cytometry - CF405M Anti-CD9 antibody [VJ1/20] (ab123624), expandable thumbnail

    Flow Cytometry - CF405M Anti-CD9 antibody [VJ1/20] (ab123624)

    ab123624, at 5 μl/106 cells, staining CD9 in normal Human peripheral blood platelets by Flow Cytometry (yellow). Unstained cells in blue.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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