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AB123624

CF405M Anti-CD9 antibody [VJ1/20]

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(2 Publications)

Mouse Monoclonal CD9 antibody - conjugated to CF®405M. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Tissue preparation containing CD9 protein.

View Alternative Names

CD9, MIC3, TSPAN29, GIG2, CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, Tetraspanin-29, p24, MRP-1, Tspan-29

1 Images
Flow Cytometry - CF405M Anti-CD9 antibody [VJ1/20] (AB123624)
  • Flow Cyt

Unknown

Flow Cytometry - CF405M Anti-CD9 antibody [VJ1/20] (AB123624)

ab123624, at 5 μl/106 cells, staining CD9 in normal Human peripheral blood platelets by Flow Cytometry (yellow). Unstained cells in blue.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

VJ1/20

Isotype

IgG2a

Conjugation

CF®405M

Excitation/Emission

Ex: 408nm, Em: 452nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Tissue preparation containing CD9 protein. The exact immunogen used to generate this antibody is proprietary information.

P21926

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/cf405m-mouse-igg2a-kappa-monoclonal-b12-8-isotype-control-ab126036'>ab126036</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

CF405M (Abs/Em Max: 408/450nm). Direct replacement for Pacific Blue dye®, BD Horizon™ V450.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD9 also known as motility-related protein-1 (MRP-1) is a transmembrane glycoprotein with a molecular weight of approximately 24 kDa. This protein is part of the tetraspanin family and shows widespread expression throughout different tissues including hematopoietic and non-hematopoietic cells. CD9 participates in numerous cellular processes by interacting with integrins and other cell surface receptors facilitating the organization of molecular complexes within the cell membrane.
Biological function summary

The CD9 protein is integral to cell adhesion migration and fusion. It forms complexes with other tetraspanins and proteins like EWI-2 and EWI-F contributing to cellular signaling and membrane compartmentalization. Additionally CD9 plays an important role in the formation and secretion of exosomes tiny vesicles emitted by cells that mediate cell-to-cell communication. These exosomes can be analyzed using tools like exosome assays and detection kits that target CD9 proteins.

Pathways

CD9 is involved in the regulation of immune response and cell morphology. It participates in pathways such as the integrin signaling pathway and the phosphatidylinositol 3-kinase (PI3K) signaling pathway. CD9 modulates these interactions by associating with proteins such as integrins and other members of the tetraspanin family influencing cellular movement and proliferation.

CD9 has connections to various conditions including cancer and infectious diseases. In cancer CD9 can influence tumor progression and metastasis while its altered expression levels have been associated with different cancer types. CD9 interacts with proteins such as integrins and CD81 in these contexts affecting cellular adhesion and migration mechanisms. In infectious diseases CD9 is involved in viral entry processes as some viruses utilize CD9 and its associated tetraspanins for entry into host cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed : 14575715, PubMed : 18541721, PubMed : 8478605). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (By similarity). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (By similarity). In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles (PubMed : 12796480). Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (By similarity). Acts as a receptor for PSG17 (By similarity). Involved in platelet activation and aggregation (PubMed : 18541721). Regulates paranodal junction formation (By similarity). Involved in cell adhesion, cell motility and tumor metastasis (PubMed : 7511626, PubMed : 8478605).
See full target information CD9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nanoscale advances 5:4435-4446 PubMed37638157

2023

Separation and isolation of CD9-positive extracellular vesicles from plasma using flow cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Karan Khanna,Nikki Salmond,Sina Halvaei,Andrew Johnson,Karla C Williams

Cell death & disease 11:217 PubMed32245975

2020

Cross organelle stress response disruption promotes gentamicin-induced proteotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Chinaemere Igwebuike,Julia Yaglom,Leah Huiting,Hui Feng,Joshua D Campbell,Zhiyong Wang,Andrea Havasi,David Pimentel,Michael Y Sherman,Steven C Borkan
View all publications
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