Rabbit Polyclonal CFA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TBCA aa 1-100.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes - |
Select an associated product type
Tubulin-folding protein; involved in the early step of the tubulin folding pathway.
Tubulin-specific chaperone A, TCP1-chaperonin cofactor A, Tubulin-folding cofactor A, CFA, TBCA
Rabbit Polyclonal CFA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TBCA aa 1-100.
IgG
Rabbit
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The CFA target often referred to as Complement Factor A is a component of the immune system with significant mechanical functions. As an integral protein it forms part of the larger complement system responsible for host defense against pathogens. Although the exact mass varies depending on post-translational modifications typical forms range between 80-100 kDa. Expression of CFA occurs predominantly within the liver and it gets secreted into the bloodstream where it participates in immune responses.
Complement Factor A participates in important immune defense processes activating the complement cascade that leads to pathogen elimination. It plays a role in the assembly of the C3 convertase complex which amplifies complement activation. Complement Factor A works in tandem with other components like C3 C4 and C5 enhancing opsonization and clearing pathogens. This connectivity within the immune response demonstrates its essential role in maintaining homeostasis and guarding against infectious agents.
Complement Factor A integrates into the classical and lectin pathways of the complement system. These pathways activate complement through antibody-antigen complexes or microbial sugars respectively. In these pathways CFA associates with proteins such as Complement Factor B and C2 where it forms active enzymatic complexes. Such interactions allow effective progression of the signaling cascade leading to potentiated immune responses and pathogen clearance.
Anomalies in Complement Factor A activity relate to conditions like autoimmune disorders and hereditary angioedema. In autoimmune disorders overactivation may lead to tissue damage while reduced function can result in infections. The protein interconnects with proteins such as Complement C3 and C1 which also influence disease pathology. Understanding CFA's detailed role provides insights into mitigating complement-related disorders through targeted interventions.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Paraffin-embedded human duodenum tissue stained for CFA using ab244456 at 1/200 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for CFA (green) using ab244456 at 4 μg/ml in ICC/IF.
All lanes: Western blot - Anti-CFA antibody (ab244456) at 0.4 µg/mL
Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2: CFA over-expression HEK-293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 13 kDa
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