AB302792

Anti-CFHR3 antibody [EPR25759-72]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Complement factor H-related protein 3 antibody. Suitable for WB, ICC/IF, IP and reacts with Human, Recombinant fragment - Human samples.

View Alternative Names

CFHL3, FHR3, CFHR3, Complement factor H-related protein 3, FHR-3, DOWN16, H factor-like protein 3

4 Images

Supplier Data

Immunocytochemistry - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Raji (human Burkitt's lymphoma B lymphocyte) cells labelling CFHR3 with ab302792 at 1/50 (10.74 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in Raji cell line and weak staining in HepG2 cell line.Low expression : HepG2 is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Lab

Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The doublet bands represent two isoforms of CFHR3 (PMID : 8428964).

Low expression : HepG2 (Human Protein Atlas)

This blot was developed using a high sensitivity ECL substrate.

All lanes:

Western blot - Anti-CFHR3 antibody [EPR25759-72] (ab302792) at 1/1000 dilution

Lane 1:

Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 35 kDa,40 kDa

false

Exposure time: 37s

Lab

Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

CFHR3 is a glycoprotein of approximately 50 kDa and detected as 37/35 kDa bands after Protein Deglycosylation Mix II treatment.(PMID : 23830046)

All lanes:

Western blot - Anti-CFHR3 antibody [EPR25759-72] (ab302792) at 1/1000 dilution

Lane 1:

Untreated human plasma at 15 µg

Lane 2:

Human plasma treated with Protein Deglycosylation Mix II at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 37 kDa

Observed band size: 50 kDa,37 kDa,35 kDa

false

Exposure time: 103s

Lab

Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human CFHR4.

Both recombinant proteins were made in-house and expressed from a mammalian expression system.

All lanes:

Western blot - Anti-CFHR3 antibody [EPR25759-72] (ab302792) at 1/1000 dilution

Lane 1:

His-tagged human CFHR3 recombinant protein at 10 ng

Lane 2:

His-tagged human CFHR4 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 37 kDa

Observed band size: 45-50 kDa

false

Exposure time: 10s

Carrier free

Anti-CFHR3 antibody [EPR25759-72] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25759-72

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Observed Mass of target (kDa) 40, 35,50, 37, 35,45-50", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Observed Mass of target (kDa) 40, 35,50, 37, 35,45-50", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Observed Mass of target (kDa) 40, 35,50, 37, 35,45-50", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Observed Mass of target (kDa) 40, 35,50, 37, 35,45-50", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Complement factor H-related protein 3 (CFHR3) also known as FHR-3 plays a role in modulating the complement system. This protein has a mass of approximately 42 kDa. CFHR3 is expressed in tissues like the liver and can be found circulating in the plasma. It belongs to the factor H protein family which participates in maintaining immune homeostasis by regulating complement activation.

Biological function summary

CFHR3 acts as an antagonist to complement factor H by competing for binding sites on target surfaces and complement components. It does not function as a standalone protein but interacts closely within a family that includes CFH and other complement factor H-related proteins. CFHR3 can potentially form complexes with similar proteins to help finely tune the activity of the complement system to prevent excessive immune response.

Pathways

CFHR3 is a part of the complement cascade system specifically the alternative pathway. It contributes to the negative regulation of this pathway which is important for preventing overactivation that could harm host tissues. The protein influences the binding of complement proteins like C3b an essential component in the complement cascade and interacts with other factor H-related proteins such as CFHR1 to modulate pathway activities.

CFHR3's regulatory role connects it to conditions like atypical hemolytic uremic syndrome (aHUS) and age-related macular degeneration (AMD). In aHUS mutations or deletions in CFHR3 sometimes in conjunction with CFHR1 can lead to uncontrolled complement activation contributing to disease progression. Similarly variations in CFHR3's sequence can affect AMD risk often in association with other complement regulatory proteins including CFH indicating its significant position in disease susceptibility through its regulatory function within the immune system.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Might be involved in complement regulation.

See full target information CFHR3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CFHR3 antibody [EPR25759-72]

Immunocytochemistry - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

Western blot - Anti-CFHR3 antibody [EPR25759-72] (AB302792)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Assay Kits

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Sample Prep & Detection Kits

Product promise