Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free (AB314306)

This data was developed using ab314305, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CFHR4 with ab314305 at 1/500 (1.038 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma of human colon. The section was incubated with ab314305 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free (AB314306)

This data was developed using ab314305, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human liver tissue labeling CFHR4 with ab314305 at 1/500 (1.038 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Weakly cytoplasmic staining on human liver (PMID : 36338737, PMID : 36338737). The section was incubated with ab314305 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free (AB314306)

This data was developed using ab314305, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human lung tissue labeling CFHR4 with ab314305 at 1/500 (1.038 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Low expression tissue : No staining on human lung. The section was incubated with ab314305 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free (AB314306)

This data was developed using ab314305, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CFHR4 with ab314305 at 1/500 (1.038 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on plasma of human kidney. The section was incubated with ab314305 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• WB

Supplier Data

Western blot - Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free (AB314306)

This data was developed using ab314305, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST CFHR4 detected a shift from 86 kDa to 75kDa band after deglycosylation treatment. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 15562282).

All lanes:

Western blot - Anti-CFHR4 antibody [EPR25760-116] (<a href='/en-us/products/primary-antibodies/cfhr4-antibody-epr25760-116-ab314305'>ab314305</a>) at 1/1000 dilution

Lane 1:

Untreated human plasma at 20 µg

Lane 2:

Human plasma treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 75 kDa,86 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free (AB314306)

This data was developed using ab314305, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : lung. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 15562282). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CFHR4 antibody [EPR25760-116] (<a href='/en-us/products/primary-antibodies/cfhr4-antibody-epr25760-116-ab314305'>ab314305</a>) at 1/1000 dilution

Lane 1:

Human serum at 20 µg

Lane 2:

Human lung lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 86 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-CFHR4 antibody [EPR25760-116] - BSA and Azide free (AB314306)

This data was developed using ab314305, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not react with human CFHR3. The recombinant proteins of CFHR4 and CFHR3 were made in house. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution

All lanes:

Western blot - Anti-CFHR4 antibody [EPR25760-116] (<a href='/en-us/products/primary-antibodies/cfhr4-antibody-epr25760-116-ab314305'>ab314305</a>) at 1/1000 dilution

Lane 1:

His-tagged human CFHR4 recombinant fragment at 20 ng

Lane 2:

His-tagged human CFHR3 recombinant protein at 20 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 86 kDa

false

Exposure time: 15s