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AB300767

Anti-CFHR4 antibody [EPR25760-31] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal CFHR4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CFHL4, FHR4, CFHR4, Complement factor H-related protein 4, FHR-4

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Sandwich ELISA - Anti-CFHR4 antibody [EPR25760-31] - BSA and Azide free (Capture) (AB300767)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CFHR4 antibody [EPR25760-31] - BSA and Azide free (Capture) (AB300767)

Sandwich ELISA of ab300766 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25760-31

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CFHR4 also known as Complement Factor H-Related 4 is a protein involved in the regulation of the complement system which plays an essential role in innate immunity. CFHR4 has a mass of approximately 86 kDa. The protein is part of the complement factor H protein family and expresses primarily in the liver and plasma. The protein features several short consensus repeat (SCR) domains that facilitate its interaction within the complement pathway therefore influencing immune system activity.
Biological function summary

CFHR4 modulates the activity of the complement system by binding to C3b a central component in complement activation. It is not a direct inhibitor but competes with Complement Factor H (CFH) for binding sites influencing factor H-mediated regulation. This positioning helps maintain a balance in complement activation and inhibition possibly preventing overactivation which could damage host tissues. CFHR4 does not form large complexes but operates closely with other complement regulators like CFH.

Pathways

CFHR4 participates in the alternative complement pathway. The alternative pathway acts as a continuous surveillance system that does not require antibody-antigen interactions for activation. CFHR4 interacts with complement components such as C3b and CFH within this pathway contributing to the control and fine-tuning of complement activity under normal physiological conditions. CFH and CFHR4 both play a role ensuring that the complement system responds accurately to threats without damaging host cells.

CFHR4 has implications in diseases like age-related macular degeneration (AMD) and atypical hemolytic uremic syndrome (aHUS). Variants or dysregulation of CFHR4 may disrupt complement control leading to inflammation and tissue damage associated with these conditions. The relationship between CFHR4 CFH and other complement components like C3b reveals the importance of balanced complement control in preventing these diseases. Understanding CFHR4's role assists researchers and clinicians in developing therapeutic strategies for conditions related to complement dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in complement regulation. Can associate with lipoproteins and may play a role in lipid metabolism.
See full target information CFHR4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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