Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Product Insider Program
Be the first to know about our latest product launches - and unlock exclusive offers and discounts.
Mouse Recombinant Monoclonal c-Fos antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
IgG2a
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Tested | Tested |
Rat | Tested | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/250 | Notes - |
Species Mouse | Dilution info 1/250 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, G0S7, FOS
Mouse Recombinant Monoclonal c-Fos antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, G0S7, FOS
IgG2a
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
N486-32
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.
The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.
C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.
Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling c-Fos with ab302667 at 1/250 (3.4 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing increased nuclear staining in HeLa cells after starvation 16 hours then treated with TPA (200 nM) for 4 h is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling c-Fos with ab302667 at 1/250 (3.4 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing increased nuclear staining in NIH/3T3 cells after starvation 16 hours then treated with TPA (200 nM) and MG-132(10 ?M) for 4 h is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling c-Fos with ab302667 at 1/1000 (0.838 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on rat cerebrum.The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling c-Fos with ab302667 at 1/2000 (0.419 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on mouse cerebrum.The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling c-Fos with ab302667 at 1/500 (1.676 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Nuclear staining on human colon.The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Data of BM-Recombinant Anti-c-Fos antibody [EPR20769] (ab214672) was in the internal.Blocking and diluting buffer and concentration: 5% NFDM/TBST
The band(s) beneath the target band are likely to be degraded target fragments (PMID: 9737957 and PMID: 20498278).
Exposure time: Lanes 1-2: 26 seconds
Lanes 3-4: 81 seconds
Lanes 5-6: 136 seconds
All lanes: Western blot - Anti-c‐Fos antibody [N486/32] (AB302667) at 1/1000 dilution
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) starved overnight, whole cell lysate 20 µg
Lane 2: HeLa starved overnight, then treated with 200nM PMA (ab120297) for 4 hours, whole cell lysate 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) starved overnight, whole cell lysate 20 µg
Lane 4: NIH/3T3 starved overnight, then treated with 200nM PMA (ab120297) and 10uM MG-132 (ab141003) for 4 hours, whole cell lysate for 4 hours, whole cell lysate 20 µg
Lane 5: PC-12 (rat adrenal gland pheochromocytoma) starved overnight, whole cell lysate 20 µg
Lane 6: PC-12 starved overnight, then treated with /ml NGF (ab9796) and 10uM MG-132 (ab141003) for 2 hours, whole cell lysate 20 µg
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 37 kDa, 55 kDa
Exposure time: 26s
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling c-Fos with ab302667 at 1/500 (1.676 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining on human cervical cancer.The section was incubated with ab302667 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody (610-4141) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com