JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB131553

Anti-CFTR antibody

5

(1 Review)

|

(9 Publications)

Rabbit Polyclonal CFTR antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 9 publications.

View Alternative Names

ABCC7, CFTR, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, Channel conductance-controlling ATPase, cAMP-dependent chloride channel

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (AB131553)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (AB131553)

ab131553 staining Cystic fibrosis transmembrane conductance regulator in MOLT4 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab131553 at 5 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab219337.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Macaque monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Have you thought about this alternative?

AB219337

Anti-CFTR antibody [CFTR/1775R]

0
0 Reviews
View product

We’re constantly upgrading our range to offer the highest quality products. We have selected this alternative product for your review because it gives improved performance, is easier and more convenient to use, or is available faster.

style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CFTR protein also known as the cystic fibrosis transmembrane conductance regulator acts as a channel that facilitates the transport of chloride ions across cell membranes. This protein plays an essential role in maintaining ion balance and hydration in various tissues. CFTR is a large glycoprotein with a molecular mass of approximately 170 kDa. It is expressed in the epithelial cells of organs such as the lungs pancreas and intestines. Its location and function underpin its significance in cellular activities and interactions.
Biological function summary

CFTR impacts the regulation of epithelial fluid movement and mucus consistency. It integrates into the cellular membrane interacting with other protein channels to form a regulatory complex. The CFTR protein's ability to transport chloride ions aids in maintaining the osmotic gradients required for proper mucus hydration. This helps prevent the build-up of thick mucus particularly in the respiratory system where it can impact breathing and infection resistance.

Pathways

The CFTR protein functions critically in electrolyte transport pathways that are connected to the regulation of ion channels and cellular signaling processes. It interacts closely with other protein channels and transporters such as ENaC forming part of the epithelial sodium channels pathway. This pathway contributes significantly to the balance of salt and water transport across epithelial surfaces. Another related pathway involves its interaction with the cAMP-dependent protein kinase (PKA) which phosphorylates CFTR modulating its activity and stability in response to cellular signals.

CFTR mutations are directly linked to cystic fibrosis a genetic condition that affects the lungs and digestive system. In cystic fibrosis dysfunctional CFTR proteins lead to the production of thick and sticky mucus resulting in respiratory and digestive complications. CFTR is also associated with congenital bilateral absence of the vas deferens (CBAVD) often related to male infertility. In both conditions protein interactions and chloride transport disruption serve as critical influences on disease progression and symptom manifestation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis (PubMed : 26823428). Mediates the transport of chloride ions across the cell membrane (PubMed : 10792060, PubMed : 11524016, PubMed : 11707463, PubMed : 12519745, PubMed : 12529365, PubMed : 12588899, PubMed : 12727866, PubMed : 15010471, PubMed : 17036051, PubMed : 1712898, PubMed : 17182731, PubMed : 19398555, PubMed : 19621064, PubMed : 22178883, PubMed : 25330774, PubMed : 26846474, PubMed : 28087700, PubMed : 8910473, PubMed : 9804160). Possesses an intrinsic ATPase activity and utilizes ATP to gate its channel; the passive flow of anions through the channel is gated by cycles of ATP binding and hydrolysis by the ATP-binding domains (PubMed : 11524016, PubMed : 15284228, PubMed : 26627831, PubMed : 8910473). The ion channel is also permeable to HCO(3)(-); selectivity depends on the extracellular chloride concentration (PubMed : 15010471, PubMed : 19019741). In vitro, mediates ATP-dependent glutathione flux (PubMed : 12727866). Exerts its function also by modulating the activity of other ion channels and transporters (PubMed : 12403779, PubMed : 22121115, PubMed : 22178883, PubMed : 27941075). Plays an important role in airway fluid homeostasis (PubMed : 16645176, PubMed : 19621064, PubMed : 26823428). Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens (PubMed : 14668433, PubMed : 16645176, PubMed : 26823428). Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex (PubMed : 17182731, PubMed : 17434346, PubMed : 27941075). Inhibits the activity of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed : 17182731). Inhibits the activity of the ENaC channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed : 17182731, PubMed : 27941075). May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7 (PubMed : 12403779). Can inhibit the chloride channel activity of ANO1 (PubMed : 22178883). Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (PubMed : 19923167, PubMed : 27714810).
See full target information CFTR
style
left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 16:937060 PubMed35966201

2022

Reduced Expression of TMEM16A Impairs Nitric Oxide-Dependent Cl Transport in Retinal Amacrine Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tyler Christopher Rodriguez,Li Zhong,Hailey Simpson,Evanna Gleason

Frontiers in physiology 13:892112 PubMed35928564

2022

Identification of Intestinal NaCl Absorptive-Anion Secretory Cells: Potential Functional Significance.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Donowitz,Rafiquel Sarker,Ruxian Lin,George McNamara,Chung Ming Tse,Varsha Singh

Frontiers in pharmacology 13:804723 PubMed35496291

2022

Effects of Maren Pills on the Intestinal Microflora and Short-Chain Fatty Acid Profile in Drug-Induced Slow Transit Constipation Model Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhan,Yong Wen,Li-Juan Du,Xiao-Xiang Wang,Shi-Yu Tang,Peng-Fei Kong,Wei-Guo Huang,Xue-Gui Tang

Scientific reports 12:1866 PubMed35115637

2022

Identification and selection of healthy spermatozoa in heterozygous carriers of the Phe508del-variant of the CFTR-gene in assisted reproduction.

Applications

Unspecified application

Species

Unspecified reactive species

Julie De Geyter,Sabina Gallati-Kraemer,Hong Zhang,Christian De Geyter

Stem cells international 2019:1024614 PubMed31191661

2019

CFTR Expression Analysis for Subtyping of Human Pancreatic Cancer Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Hennig,Laura Wolf,Beatrix Jahnke,Heike Polster,Therese Seidlitz,Kristin Werner,Daniela E Aust,Jochen Hampe,Marius Distler,Jürgen Weitz,Daniel E Stange,Thilo Welsch

PloS one 13:e0201184 PubMed30044876

2018

Inhibition of endocytosis suppresses the nitric oxide-dependent release of Cl- in retinal amacrine cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vernon K Dunn,Evanna Gleason

Journal of neurophysiology 118:2842-2852 PubMed28835528

2017

A role for the cystic fibrosis transmembrane conductance regulator in the nitric oxide-dependent release of Cl from acidic organelles in amacrine cells.

Applications

Unspecified application

Species

Unspecified reactive species

Vijai Krishnan,J Wesley Maddox,Tyler Rodriguez,Evanna Gleason

JCI insight 2:e86492 PubMed28097227

2017

A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hajime Uchida,Masakazu Machida,Takumi Miura,Tomoyuki Kawasaki,Takuya Okazaki,Kengo Sasaki,Seisuke Sakamoto,Noriaki Ohuchi,Mureo Kasahara,Akihiro Umezawa,Hidenori Akutsu

Biochimica et biophysica acta 1853:892-903 PubMed25661196

2015

SERCA and PMCA pumps contribute to the deregulation of Ca2+ homeostasis in human CF epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Réginald Philippe,Fabrice Antigny,Paul Buscaglia,Caroline Norez,Frédéric Becq,Maud Frieden,Olivier Mignen
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB217888

Anti-CFTR antibody [CFTR/1643]

primary-antibodies

cftr-antibody-cftr-1643-ab217888

0

(0 reviews)

Alternative Product
Primary Antibodies

AB269738

Anti-CFTR antibody [CFTR/1643] - BSA and Azide free

primary-antibodies

cftr-antibody-cftr-1643-bsa-and-azide-free-ab269738

0

(0 reviews)

Alternative Product
Primary Antibodies

AB234037

Anti-CFTR antibody [SPM176] - BSA and Azide free

primary-antibodies

cftr-antibody-spm176-bsa-and-azide-free-ab234037

0

(0 reviews)

Alternative Product
Primary Antibodies

AB181782

Anti-CFTR antibody

primary-antibodies

cftr-antibody-ab181782

3

(1 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com