AB181782

Anti-CFTR antibody

Name: Anti-CFTR antibody (ab181782) | Abcam
Brand: Abcam Limited
SKU: AB181782
Price: 430 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal CFTR antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Human, Transfected cell line - Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human CFTR.

View Alternative Names

ABCC7, CFTR, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, Channel conductance-controlling ATPase, cAMP-dependent chloride channel

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (AB181782)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (AB181782)

Immunofluorescent analysis of BHK cells expressing wild-type (wt) or mutated (rΔF508) CFTR, labeling CFTR with ab181782.

Supplier Data

Western blot - Anti-CFTR antibody (AB181782)

All lanes:

Western blot - Anti-CFTR antibody (ab181782) at 1/500 dilution

Lane 1:

BHK cells

Lane 2:

BHK cells transfected with CFTR-3HA tag

Lane 3:

Immunprecipitated wt CFTR from BHK cells

Predicted band size: 168 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human CFTR. The exact immunogen used to generate this antibody is proprietary information.

P13569

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CFTR protein also known as the cystic fibrosis transmembrane conductance regulator acts as a channel that facilitates the transport of chloride ions across cell membranes. This protein plays an essential role in maintaining ion balance and hydration in various tissues. CFTR is a large glycoprotein with a molecular mass of approximately 170 kDa. It is expressed in the epithelial cells of organs such as the lungs pancreas and intestines. Its location and function underpin its significance in cellular activities and interactions.

Biological function summary

CFTR impacts the regulation of epithelial fluid movement and mucus consistency. It integrates into the cellular membrane interacting with other protein channels to form a regulatory complex. The CFTR protein's ability to transport chloride ions aids in maintaining the osmotic gradients required for proper mucus hydration. This helps prevent the build-up of thick mucus particularly in the respiratory system where it can impact breathing and infection resistance.

Pathways

The CFTR protein functions critically in electrolyte transport pathways that are connected to the regulation of ion channels and cellular signaling processes. It interacts closely with other protein channels and transporters such as ENaC forming part of the epithelial sodium channels pathway. This pathway contributes significantly to the balance of salt and water transport across epithelial surfaces. Another related pathway involves its interaction with the cAMP-dependent protein kinase (PKA) which phosphorylates CFTR modulating its activity and stability in response to cellular signals.

CFTR mutations are directly linked to cystic fibrosis a genetic condition that affects the lungs and digestive system. In cystic fibrosis dysfunctional CFTR proteins lead to the production of thick and sticky mucus resulting in respiratory and digestive complications. CFTR is also associated with congenital bilateral absence of the vas deferens (CBAVD) often related to male infertility. In both conditions protein interactions and chloride transport disruption serve as critical influences on disease progression and symptom manifestation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis (PubMed : 26823428). Mediates the transport of chloride ions across the cell membrane (PubMed : 10792060, PubMed : 11524016, PubMed : 11707463, PubMed : 12519745, PubMed : 12529365, PubMed : 12588899, PubMed : 12727866, PubMed : 15010471, PubMed : 17036051, PubMed : 1712898, PubMed : 17182731, PubMed : 19398555, PubMed : 19621064, PubMed : 22178883, PubMed : 25330774, PubMed : 26846474, PubMed : 28087700, PubMed : 8910473, PubMed : 9804160). Possesses an intrinsic ATPase activity and utilizes ATP to gate its channel; the passive flow of anions through the channel is gated by cycles of ATP binding and hydrolysis by the ATP-binding domains (PubMed : 11524016, PubMed : 15284228, PubMed : 26627831, PubMed : 8910473). The ion channel is also permeable to HCO(3)(-); selectivity depends on the extracellular chloride concentration (PubMed : 15010471, PubMed : 19019741). In vitro, mediates ATP-dependent glutathione flux (PubMed : 12727866). Exerts its function also by modulating the activity of other ion channels and transporters (PubMed : 12403779, PubMed : 22121115, PubMed : 22178883, PubMed : 27941075). Plays an important role in airway fluid homeostasis (PubMed : 16645176, PubMed : 19621064, PubMed : 26823428). Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens (PubMed : 14668433, PubMed : 16645176, PubMed : 26823428). Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex (PubMed : 17182731, PubMed : 17434346, PubMed : 27941075). Inhibits the activity of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed : 17182731). Inhibits the activity of the ENaC channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed : 17182731, PubMed : 27941075). May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7 (PubMed : 12403779). Can inhibit the chloride channel activity of ANO1 (PubMed : 22178883). Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (PubMed : 19923167, PubMed : 27714810, PubMed : 29393851).

See full target information CFTR

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 23:259-270 PubMed36226596

2023

Deletion of sphingosine kinase 2 attenuates cigarette smoke-mediated chronic obstructive pulmonary disease-like symptoms by reducing lung inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanhui Chen,Yongrong Zhang,Cheng Rao,Jieyun Huang,Qiong Qing

Frontiers in pharmacology 13:804723 PubMed35496291

2022

Effects of Maren Pills on the Intestinal Microflora and Short-Chain Fatty Acid Profile in Drug-Induced Slow Transit Constipation Model Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhan,Yong Wen,Li-Juan Du,Xiao-Xiang Wang,Shi-Yu Tang,Peng-Fei Kong,Wei-Guo Huang,Xue-Gui Tang

Computational and mathematical methods in medicine 2022:8268067 PubMed35126637

2022

A Hyperglycemic Microenvironment Inhibits Tendon-to-Bone Healing through the let-7b-5p/CFTR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Cao,Junyi Hong,Feicheng Qi,Bo Zheng,Guofang Chen,Binjia Yu,Fusheng Ye

Biochemical and biophysical research communications 551:21-26 PubMed33714755

2021

LncRNA MITA1 promotes gefitinib resistance by inducing autophagy in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Hu,Shu-Wen Dong,Yinghua Pei,Juan Wang,Jie Zhang,Xiu-Ping Wei

The American journal of pathology 190:977-993 PubMed32084371

2020

Phenotypic Characterization and Comparison of Cystic Fibrosis Rat Models Generated Using CRISPR/Cas9 Gene Editing.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra McCarron,Patricia Cmielewski,Nicole Reyne,Chantelle McIntyre,John Finnie,Fiona Craig,Nathan Rout-Pitt,Juliette Delhove,John E Schjenken,Hon Y Chan,Bernadette Boog,Emma Knight,Rodney C Gilmore,Wanda K O'Neal,Richard C Boucher,David Parsons,Martin Donnelley

Bioscience reports 40: PubMed31894837

2020

Effect of lentivirus-mediated CFTR overexpression on oxidative stress injury and inflammatory response in the lung tissue of COPD mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Xu,Huimin Huang,Xiangyi Yin,Hongmei Fang,Xiaoyue Shen

Molecular therapy : the journal of the American So 27:1749-1757 PubMed31351782

2019

Steric Inhibition of 5' UTR Regulatory Elements Results in Upregulation of Human CFTR.

Applications

Unspecified application

Species

Unspecified reactive species

Shruti Sasaki,Rachel Sun,Huynh-Hoa Bui,Jeff R Crosby,Brett P Monia,Shuling Guo

Journal of the Association for Research in Otolary 19:381-399 PubMed29869046

2018

Characterization of Adult Vestibular Organs in 11 CreER Mouse Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer S Stone,Serena R Wisner,Stephanie A Bucks,Marcia M Mellado Lagarde,Brandon C Cox

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-CFTR antibody

Immunocytochemistry/ Immunofluorescence - Anti-CFTR antibody (AB181782)

Western blot - Anti-CFTR antibody (AB181782)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise