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AB217888

Anti-CFTR antibody [CFTR/1643]

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(1 Publication)

Mouse Monoclonal CFTR antibody. Suitable for Protein Array, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CFTR aa 250-400.

View Alternative Names

ABCC7, CFTR, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, Channel conductance-controlling ATPase, cAMP-dependent chloride channel

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [CFTR/1643] (AB217888)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [CFTR/1643] (AB217888)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human pancreas tissue labeling CFTR with ab217888 at 2 μg/mL.

Protein Array - Anti-CFTR antibody [CFTR/1643] (AB217888)
  • Protein Array

Unknown

Protein Array - Anti-CFTR antibody [CFTR/1643] (AB217888)

ab217888 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CFTR/1643

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P, Protein Array

applications

Immunogen

Recombinant Fragment Protein within Human CFTR aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

P13569

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
ab217888 is purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CFTR protein also known as the cystic fibrosis transmembrane conductance regulator acts as a channel that facilitates the transport of chloride ions across cell membranes. This protein plays an essential role in maintaining ion balance and hydration in various tissues. CFTR is a large glycoprotein with a molecular mass of approximately 170 kDa. It is expressed in the epithelial cells of organs such as the lungs pancreas and intestines. Its location and function underpin its significance in cellular activities and interactions.
Biological function summary

CFTR impacts the regulation of epithelial fluid movement and mucus consistency. It integrates into the cellular membrane interacting with other protein channels to form a regulatory complex. The CFTR protein's ability to transport chloride ions aids in maintaining the osmotic gradients required for proper mucus hydration. This helps prevent the build-up of thick mucus particularly in the respiratory system where it can impact breathing and infection resistance.

Pathways

The CFTR protein functions critically in electrolyte transport pathways that are connected to the regulation of ion channels and cellular signaling processes. It interacts closely with other protein channels and transporters such as ENaC forming part of the epithelial sodium channels pathway. This pathway contributes significantly to the balance of salt and water transport across epithelial surfaces. Another related pathway involves its interaction with the cAMP-dependent protein kinase (PKA) which phosphorylates CFTR modulating its activity and stability in response to cellular signals.

CFTR mutations are directly linked to cystic fibrosis a genetic condition that affects the lungs and digestive system. In cystic fibrosis dysfunctional CFTR proteins lead to the production of thick and sticky mucus resulting in respiratory and digestive complications. CFTR is also associated with congenital bilateral absence of the vas deferens (CBAVD) often related to male infertility. In both conditions protein interactions and chloride transport disruption serve as critical influences on disease progression and symptom manifestation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis (PubMed : 26823428). Mediates the transport of chloride ions across the cell membrane (PubMed : 10792060, PubMed : 11524016, PubMed : 11707463, PubMed : 12519745, PubMed : 12529365, PubMed : 12588899, PubMed : 12727866, PubMed : 15010471, PubMed : 17036051, PubMed : 1712898, PubMed : 17182731, PubMed : 19398555, PubMed : 19621064, PubMed : 22178883, PubMed : 25330774, PubMed : 26846474, PubMed : 28087700, PubMed : 8910473, PubMed : 9804160). Possesses an intrinsic ATPase activity and utilizes ATP to gate its channel; the passive flow of anions through the channel is gated by cycles of ATP binding and hydrolysis by the ATP-binding domains (PubMed : 11524016, PubMed : 15284228, PubMed : 26627831, PubMed : 8910473). The ion channel is also permeable to HCO(3)(-); selectivity depends on the extracellular chloride concentration (PubMed : 15010471, PubMed : 19019741). In vitro, mediates ATP-dependent glutathione flux (PubMed : 12727866). Exerts its function also by modulating the activity of other ion channels and transporters (PubMed : 12403779, PubMed : 22121115, PubMed : 22178883, PubMed : 27941075). Plays an important role in airway fluid homeostasis (PubMed : 16645176, PubMed : 19621064, PubMed : 26823428). Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens (PubMed : 14668433, PubMed : 16645176, PubMed : 26823428). Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex (PubMed : 17182731, PubMed : 17434346, PubMed : 27941075). Inhibits the activity of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed : 17182731). Inhibits the activity of the ENaC channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed : 17182731, PubMed : 27941075). May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7 (PubMed : 12403779). Can inhibit the chloride channel activity of ANO1 (PubMed : 22178883). Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (PubMed : 19923167, PubMed : 27714810).
See full target information CFTR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational pediatrics 13:1190-1200 PubMed39144436

2024

Screening for biomarkers of tuberous sclerosis complex-associated epilepsy: a bioinformatics analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongsheng Sun,Haonan Ji,Liqin Xu,Ruiyin Gu,Pasquale Striano,Gavin P Winston,Bin Li,Hui Zhou
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