Anti-CFTR antibody [EPR29790-578]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [EPR29790-578] (AB325171)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CFTR with ab325171 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on apical pole of proximal tubule in rat kidney.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [EPR29790-578] (AB325171)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CFTR with ab325171 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on apical pole of proximal tubule in mouse kidney (PMID : 28701694).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-CFTR antibody [EPR29790-578] (AB325171)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse pancreas tissue staining CFTR with ab325171 at a 1 : 2000 (0.259 ug/ml) dilution, ab323181 anti-TMEM16A used at 1 : 1000 (0.532 ug/ml) dilution and ab181547 anti-Insulin used at a 1 : 20000 (0.053 ug/ml) dilution.

Panel A : anti-CFTR (green; Opal™520), anti-TMEM16A (magenta; Opal™690) and anti-Insulin (gray; Opal™570) on mouse pancreas.
Panel B : anti-CFTR staining centroacinar cells in mouse pancreas.
Panel C : anti-TMEM16A staining epithelium in mouse pancreas.
Panel D : anti-Insulin staining islet cells in mouse pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325171, ab323181 and ab181547 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [EPR29790-578] (AB325171)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling CFTR with ab325171 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on epithelial cells of mouse colon (PMID : 12923244).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [EPR29790-578] (AB325171)

Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling CFTR with ab325171 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on centroacinar cells of rat pancreas (PMID : 30389171).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [EPR29790-578] (AB325171)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling CFTR with ab325171 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on epithelial cells of rat colon.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [EPR29790-578] (AB325171)

Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling CFTR with ab325171 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on centroacinar cells of mouse pancreas.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-CFTR antibody [EPR29790-578] (AB325171)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

CFTR is a glycoprotein of approximately 130-220 kDa and is detected as a 150 kDa band after treatment with the deglycosylation enzyme Peptide : N-glycosidase F (PNGase F).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-CFTR antibody [EPR29790-578] (ab325171) at 1/1000 dilution

Lane 1:

Untreated mouse lung tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-220 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-CFTR antibody [EPR29790-578] (AB325171)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle, heart.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 38928073; PMID : 28242698 ).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lane 1 : 103 seconds; Lanes 2-6 : 180 seconds

All lanes:

Western blot - Anti-CFTR antibody [EPR29790-578] (ab325171) at 1/1000 dilution

Lane 1:

Mouse colon tissue lysate at 48 µg

Lane 2:

Mouse kidney tissue lysate at 48 µg

Lane 3:

Mouse testis tissue lysate at 48 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 48 µg

Lane 5:

Mouse heart tissue lysate at 48 µg

Lane 6:

Mouse lung tissue lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-220 kDa,124 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [EPR29790-578] (AB325171)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling CFTR with ab325171 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on rat skeletal muscle.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [EPR29790-578] (AB325171)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CFTR with ab325171 at 1/2000 (0.259 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on mouse skeletal muscle.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins