AB270238

Anti-CFTR antibody [M3A7]

Recombinant
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(3 Publications)

Mouse Recombinant Monoclonal CFTR antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CFTR.

View Alternative Names

ABCC7, CFTR, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, Channel conductance-controlling ATPase, cAMP-dependent chloride channel

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [M3A7] (AB270238)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [M3A7] (AB270238)

Formalin-fixed, paraffin embedded human pancreas tissue stained for CFTR using ab270238 at 2 μg/ml in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M3A7

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CFTR. The exact immunogen used to generate this antibody is proprietary information.

P13569

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from Bioreactor Concentrate.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The CFTR protein also known as the cystic fibrosis transmembrane conductance regulator acts as a channel that facilitates the transport of chloride ions across cell membranes. This protein plays an essential role in maintaining ion balance and hydration in various tissues. CFTR is a large glycoprotein with a molecular mass of approximately 170 kDa. It is expressed in the epithelial cells of organs such as the lungs pancreas and intestines. Its location and function underpin its significance in cellular activities and interactions.

Biological function summary

CFTR impacts the regulation of epithelial fluid movement and mucus consistency. It integrates into the cellular membrane interacting with other protein channels to form a regulatory complex. The CFTR protein's ability to transport chloride ions aids in maintaining the osmotic gradients required for proper mucus hydration. This helps prevent the build-up of thick mucus particularly in the respiratory system where it can impact breathing and infection resistance.

Pathways

The CFTR protein functions critically in electrolyte transport pathways that are connected to the regulation of ion channels and cellular signaling processes. It interacts closely with other protein channels and transporters such as ENaC forming part of the epithelial sodium channels pathway. This pathway contributes significantly to the balance of salt and water transport across epithelial surfaces. Another related pathway involves its interaction with the cAMP-dependent protein kinase (PKA) which phosphorylates CFTR modulating its activity and stability in response to cellular signals.

CFTR mutations are directly linked to cystic fibrosis a genetic condition that affects the lungs and digestive system. In cystic fibrosis dysfunctional CFTR proteins lead to the production of thick and sticky mucus resulting in respiratory and digestive complications. CFTR is also associated with congenital bilateral absence of the vas deferens (CBAVD) often related to male infertility. In both conditions protein interactions and chloride transport disruption serve as critical influences on disease progression and symptom manifestation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Epithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis (PubMed : 26823428). Mediates the transport of chloride ions across the cell membrane (PubMed : 10792060, PubMed : 11524016, PubMed : 11707463, PubMed : 12519745, PubMed : 12529365, PubMed : 12588899, PubMed : 12727866, PubMed : 15010471, PubMed : 17036051, PubMed : 1712898, PubMed : 17182731, PubMed : 19398555, PubMed : 19621064, PubMed : 22178883, PubMed : 25330774, PubMed : 26846474, PubMed : 28087700, PubMed : 8910473, PubMed : 9804160). Possesses an intrinsic ATPase activity and utilizes ATP to gate its channel; the passive flow of anions through the channel is gated by cycles of ATP binding and hydrolysis by the ATP-binding domains (PubMed : 11524016, PubMed : 15284228, PubMed : 26627831, PubMed : 8910473). The ion channel is also permeable to HCO(3)(-); selectivity depends on the extracellular chloride concentration (PubMed : 15010471, PubMed : 19019741). In vitro, mediates ATP-dependent glutathione flux (PubMed : 12727866). Exerts its function also by modulating the activity of other ion channels and transporters (PubMed : 12403779, PubMed : 22121115, PubMed : 22178883, PubMed : 27941075). Plays an important role in airway fluid homeostasis (PubMed : 16645176, PubMed : 19621064, PubMed : 26823428). Contributes to the regulation of the pH and the ion content of the airway surface fluid layer and thereby plays an important role in defense against pathogens (PubMed : 14668433, PubMed : 16645176, PubMed : 26823428). Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex (PubMed : 17182731, PubMed : 17434346, PubMed : 27941075). Inhibits the activity of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed : 17182731). Inhibits the activity of the ENaC channel containing subunits SCNN1D, SCNN1B and SCNN1G, but not of the ENaC channel containing subunits SCNN1A, SCNN1B and SCNN1G (PubMed : 17182731, PubMed : 27941075). May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7 (PubMed : 12403779). Can inhibit the chloride channel activity of ANO1 (PubMed : 22178883). Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (PubMed : 19923167, PubMed : 27714810, PubMed : 29393851).

See full target information CFTR

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Hepatology communications 9: PubMed40366121

2025

Single-cell transcription reveals hepatocyte-to-cholangiocyte reprogramming and biliary gene profile in biliary atresia.

Applications

Unspecified application

Species

Unspecified reactive species

Lingdu Meng,Min Du,Haodong Li,Fanyang Kong,Jiajian Yang,Rui Dong,Shan Zheng,Gong Chen,Zhen Shen,Junfeng Wang

Communications biology 7:1062 PubMed39215205

2024

UNSEG: unsupervised segmentation of cells and their nuclei in complex tissue samples.

Applications

Unspecified application

Species

Unspecified reactive species

Bogdan Kochetov,Phoenix D Bell,Paulo S Garcia,Akram S Shalaby,Rebecca Raphael,Benjamin Raymond,Brian J Leibowitz,Karen Schoedel,Rhonda M Brand,Randall E Brand,Jian Yu,Lin Zhang,Brenda Diergaarde,Robert E Schoen,Aatur Singhi,Shikhar Uttam

Nature communications 13:3586 PubMed35739107

2022

Macrocycle-stabilization of its interaction with 14-3-3 increases plasma membrane localization and activity of CFTR.

Applications

Unspecified application

Species

Unspecified reactive species

Loes M Stevers,Madita Wolter,Graeme W Carlile,Dwight Macdonald,Luc Richard,Frank Gielkens,John W Hanrahan,David Y Thomas,Sai Kumar Chakka,Mark L Peterson,Helmut Thomas,Luc Brunsveld,Christian Ottmann

View all publications

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Product promise

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Anti-CFTR antibody [M3A7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CFTR antibody [M3A7] (AB270238)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise