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AB259503

Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal GLHA antibody. Carrier free. Suitable for sELISA, IHC-P, WB and reacts with Mouse samples.

View Alternative Names

Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, FSH-alpha, LSH-alpha, TSH-alpha, Cga

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture) (AB259503)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture) (AB259503)

Immunohistochemical analysis of paraffin-embedded mouse pituitary labeling CGA/hCG alpha with ab259503 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse pituitary.
The section was incubated with ab259503 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture) (AB259503)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture) (AB259503)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle labeling CGA/hCG alpha with ab259503 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative Control : no staining on mouse skeletal muscle.
The section was incubated with ab259503 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Western blot - Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture) (AB259503)
  • WB

Lab

Western blot - Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture) (AB259503)

Negative controls :  ovary, pancreas, brain, liver and heart.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture) (ab259503) at 1/1000 dilution

Lane 1:

Mouse pituitary tissue lysate at 20 µg

Lane 2:

Mouse ovary tissue lysate at 20 µg

Lane 3:

Mouse pancreas tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa

false

Exposure time: 1s

Sandwich ELISA - Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture) (AB259503)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CGA/hCG alpha antibody [EPR19238-39] - BSA and Azide free (Capture) (AB259503)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab243671).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19238-39

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

AB272371

Anti-Chorionic Gonadotropin antibody [EPR23060-32] - BSA and Azide free (Detector)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

ab259503 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse hCG alpha. The recommended pair for sandwich ELISA is:
Capture: ab259503, Mouse hCG alpha Capture Antibody (unconjugated)
Detector: ab259504, Mouse hCG alpha Detector Antibody (unconjugated)
The reference range value is 78 - 5000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CGA also known as the hCG alpha subunit is a glycoprotein hormone free alpha subunit. Its molecular mass is approximately 15-22 kDa. It is a part of the human chorionic gonadotropin (hCG) protein. It is expressed in the placenta and various neoplastic tissues. CGA along with the beta subunit forms the complete structure of hCG which plays a significant role in signaling for the reproductive processes.
Biological function summary

This alpha subunit plays a fundamental role in forming the heterodimer with different beta subunits of hCG TSH LH and FSH resulting in various glycoprotein hormones. These hormones are essential for maintaining pregnancy fetal development and regulating the menstrual cycle. The CGA protein is key to the activity of the hCG protein which significantly impacts fetal and trophoblastic growth during pregnancy. The heterodimeric complex to which it contributes assigns it unique biological roles that differ depending on the linked beta subunit.

Pathways

The CGA subunit functions within the reproductive hormone signaling pathway. The uniform alpha subunit pairs with distinct beta subunits such as LH beta or FSH beta influencing different signaling pathways. It plays a vital part in the hormonal regulation pathway modulating endocrine and reproductive processes. The related proteins include the beta subunits like hCG beta LH beta and FSH beta which pair with CGA to perform distinct biological functions in different tissues.

CGA's expression relates to trophoblastic diseases and certain types of cancer such as choriocarcinoma. Aberrant expression or regulation can be seen in these conditions linking it to tumorigenesis and abnormal reproductive disorders. Choriocarcinoma significantly involves hCG protein and its subunits where CGA influences the progression by contributing to this hormone's abnormal activity. Elevated levels of CGA can correlate with the progression of these diseases serving as a clinical marker for diagnosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH and follitropin/follicle stimulating hormone/FSH. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.
See full target information Cga
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com