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AB269757

Anti-CGA/hCG alpha antibody [LHa/756] - BSA and Azide free

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Mouse Monoclonal GLHA antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CGA.

View Alternative Names

Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha, CGA

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [LHa/756] - BSA and Azide free (AB269757)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [LHa/756] - BSA and Azide free (AB269757)

Immunohistochemical analysis of paraffin embedded, formalin fixed Human pituitary tissue labeling CGA/hCG alpha using ab219296 at 2 μg/mL.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219296).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LHa/756

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CGA.

P01215

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Alternative Products

Primary Antibodies

AB219296

Anti-CGA/hCG alpha antibody [LHa/756]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGA/hCG alpha antibody [LHa/756] (AB219296)

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Complementary Products

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Human hCG ELISA Kit

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Primary Antibodies

AB155958

Anti-TSH beta antibody [EPR8198]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH beta antibody [EPR8198] (AB155958)

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Primary Antibodies

AB131170

Anti-hCG beta antibody [EPHCGR2]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hCG beta antibody [EPHCGR2] (AB131170)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab269757 is a carrier free version of ab219296.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CGA also known as the hCG alpha subunit is a glycoprotein hormone free alpha subunit. Its molecular mass is approximately 15-22 kDa. It is a part of the human chorionic gonadotropin (hCG) protein. It is expressed in the placenta and various neoplastic tissues. CGA along with the beta subunit forms the complete structure of hCG which plays a significant role in signaling for the reproductive processes.
Biological function summary

This alpha subunit plays a fundamental role in forming the heterodimer with different beta subunits of hCG TSH LH and FSH resulting in various glycoprotein hormones. These hormones are essential for maintaining pregnancy fetal development and regulating the menstrual cycle. The CGA protein is key to the activity of the hCG protein which significantly impacts fetal and trophoblastic growth during pregnancy. The heterodimeric complex to which it contributes assigns it unique biological roles that differ depending on the linked beta subunit.

Pathways

The CGA subunit functions within the reproductive hormone signaling pathway. The uniform alpha subunit pairs with distinct beta subunits such as LH beta or FSH beta influencing different signaling pathways. It plays a vital part in the hormonal regulation pathway modulating endocrine and reproductive processes. The related proteins include the beta subunits like hCG beta LH beta and FSH beta which pair with CGA to perform distinct biological functions in different tissues.

CGA's expression relates to trophoblastic diseases and certain types of cancer such as choriocarcinoma. Aberrant expression or regulation can be seen in these conditions linking it to tumorigenesis and abnormal reproductive disorders. Choriocarcinoma significantly involves hCG protein and its subunits where CGA influences the progression by contributing to this hormone's abnormal activity. Elevated levels of CGA can correlate with the progression of these diseases serving as a clinical marker for diagnosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Shared alpha chain of the active heterodimeric glycoprotein hormones thyrotropin/thyroid stimulating hormone/TSH, lutropin/luteinizing hormone/LH, follitropin/follicle stimulating hormone/FSH and choriogonadotropin/CG. These hormones bind specific receptors on target cells that in turn activate downstream signaling pathways.
See full target information CGA
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Product promise

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