Anti-cGAS antibody [EPR26492-84]
- 20ul selling size
- RabMAb
- Recombinant
- What is this?
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(7 Publications)
Anti-cGAS antibody [EPR26492-84] (ab302617) is a rabbit monoclonal antibody detecting cGAS in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
View Alternative Names
C6orf150, MB21D1, CGAS, Cyclic GMP-AMP synthase, cGAMP synthase, cGAS, h-cGAS, 2'3'-cGAMP synthase, Mab-21 domain-containing protein 1
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-cGAS antibody [EPR26492-84] (AB302617)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized No-GFP-CD16.NK-92 (human lymphoblast natural killer cell; nk cell) cells labelling cGAS with ab302617 at 1/50 (10.0 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic and membranous staining in No-GFP-CD16.NK-92 cell lineNegative control : HCT 116 (PMID : 33790360) is observed. was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-cGAS antibody [EPR26492-84] (AB302617)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell, Left) / No-GFP-CD16.NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell, Right) cells labelling cGAS with ab302617 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : HCT 116 (PMID : 33790360)
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-cGAS antibody [EPR26492-84] (AB302617)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HT-29 (Human colorectal adenocarcinoma epithelial cell) cells labelling cGAS with ab302617 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
- Flow Cyt (Intra)
Supplier Data
Flow Cytometry (Intracellular) - Anti-cGAS antibody [EPR26492-84] (AB302617)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling cGAS with ab302617 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
- WB
Supplier Data
Western blot - Anti-cGAS antibody [EPR26492-84] (AB302617)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : HCT 116 (PMID : 33790360) HT-29 lysate was freshly made and used for Western blotting immediately to minimize protein degradation. The bands between 40 kDa and 23 kDa are likely to be degraded target fragments. Exposure time : 26 seconds
All lanes:
Western blot - Anti-cGAS antibody [EPR26492-84] (ab302617) at 1/1000 dilution
Lane 1:
HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate 20 µg
Lane 2:
HCT 116 (human colorectal carcinoma epithelial cell), whole cell lysate 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 62 kDa
false
Exposure time: 26s
- WB
Supplier Data
Western blot - Anti-cGAS antibody [EPR26492-84] (AB302617)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysate was freshly made and used for Western blotting immediately to minimize protein degradation. The identity of the bands above 250 kDa are unknown. Exposure time : 37 seconds
All lanes:
Western blot - Anti-cGAS antibody [EPR26492-84] (ab302617) at 1/1000 dilution
All lanes:
Hela (human cervix adenocarcinoma epithelial cell), whole cell lysate 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Observed band size: 62 kDa
false
Exposure time: 37s
- WB
Supplier Data
Western blot - Anti-cGAS antibody [EPR26492-84] (AB302617)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysate was freshly made and used for Western blotting immediately to minimize protein degradation. The identity of the higher MW band at approximately 250 kDa is unknown. Exposure time : 15 seconds
All lanes:
Western blot - Anti-cGAS antibody [EPR26492-84] (ab302617) at 1/1000 dilution
All lanes:
No-GFP-CD16.NK-92 (human malignant non-Hodgkins lymphoma natural killer cell), whole cell lysate 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Observed band size: 62 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-cGAS antibody [EPR26492-84] (AB302617)
Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysate was freshly made and used for Western blotting immediately to minimize protein degradation. The band beneath the target band (23 kDa) is likely to be degraded target fragment. Exposure time : 59 seconds
All lanes:
Western blot - Anti-cGAS antibody [EPR26492-84] (ab302617) at 1/1000 dilution
All lanes:
MCF-7 (human breast adenocarcinoma epithelial cell), whole cell lysate 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 62 kDa
false
Exposure time: 59s
Related conjugates and formulations (1)
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Anti-cGAS antibody [EPR26492-84] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-cGAS antibody [EPR26492-84] (ab302617) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
What is the molecular weight of cGAS?
Anti-cGAS [EPR26492-84] (ab302617) specifically detects a band for cGAS (UniProt: Q8N884) at a molecular weight of 59kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR26492-84] also available for your convenience: ab302617, Carrier free - ab302618
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CGAS plays a fundamental role in the immune response by activating the STING pathway. cGAS when bound to DNA forms a complex with DNA as part of its mechanistic function which then catalyzes the synthesis of cGAMP. The cGAMP produced acts as a signaling molecule that activates the STING protein leading to subsequent immune signaling cascades. This activation results in the production of type I interferons and other inflammatory cytokines essential for mounting an effective antiviral response.
Pathways
CGAS operates within important signaling pathways such as the cGAS-STING pathway and the type I interferon pathway. This protein works closely with STING located on the endoplasmic reticulum as a direct effector. The connection between cGAS and STING is important in detecting and responding to cytosolic DNA and initiating immune signaling leading to activation of downstream transcription factors like IRF3 and NF-kB which promote gene expression driving immune responses.
Product protocols
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Target data
Publications (7)
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Cell communication and signaling : CCS 23:306 PubMed40597187
2025
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iScience 28:112391 PubMed40322081
2025
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Journal of neuroinflammation 22:11 PubMed39833906
2025
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Experimental hematology & oncology 13:77 PubMed39103908
2024
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Frontiers in cell and developmental biology 12:1422746 PubMed39050892
2024
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Acta pharmaceutica Sinica. B 14:1742-1758 PubMed38572099
2024
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Journal of cancer research and clinical oncology 149:6315-6328 PubMed36745223
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com