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AB249503

Anti-cGMP antibody [EPR10355] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Guanosine, cyclic 3',5'-(hydrogen phosphate), monosodium salt antibody. Carrier free. Suitable for cELISA and reacts with Chemical samples. Cited in 1 publication.

View Alternative Names

Cyclic GMP, Cyclic guanosine monophosphate, Guanosine 3 5 Cyclic Monophosphate, cGMP

2 Images
Competitive ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (AB249503)
  • cELISA

Unknown

Competitive ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (AB249503)

This data was developed using ab169753, the same antibody clone in a different buffer formulation.2 μg/ml of Goat anti-rabbit IgG (50 μl/well) was coated into 96-wells. After washing with 0.1 % Tween-20, 0.1 μg/ml of ab169753 (50 μl/well) was added and incubated for 1 hour. After washing, 0, 8, 48, 64, 96, 128, 256 and 1000 ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP and cCMP (25 μl/well) were added along with 25 μl of the 1/50 diluted HRP conjugated cGMP. After washing, TMB was used to develop the color.

Competitive ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (AB249503)
  • cELISA

Unknown

Competitive ELISA - Anti-cGMP antibody [EPR10355] - BSA and Azide free (AB249503)

This data was developed using ab169753, the same antibody clone in a different buffer formulation.2μg/ml of goat-anti-rabbit IgG (50μl/well) was coated into 96-wells. After washing with 0.1% Tween-20, 0.2μg/ml of ab169753 (50 μl / well) was added and incubated for 1hr. Then 62.5ng/ml of cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP, cIMP, cCMP and H2O (25 μl / well each) were added, respectively. Finally 25 μl of 1/50 diluted HRP conjugated cGMP was added in each well. TMB was used to develop the color.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10355

Isotype

IgG

Carrier free

Yes

Applications

cELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "cELISA" : {"fullname" : "Competitive ELISA", "shortname":"cELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "cELISA-species-checked": "testedAndGuaranteed", "cELISA-species-dilution-info": "", "cELISA-species-notes": "<p></p>" } } }
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Product details

ab249503 is the carrier-free version of ab169753.

The product is specific for cGMP and as tested it does not cross-react with cAMP, AMP, ADP, ATP, cGMP, GMP, GDP, GTP and cCMP.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cyclic guanosine monophosphate commonly known as cGMP is a second messenger important in various physiological processes. It has a molecular mass of 345.2 g/mol. cGMP is produced as a result of the conversion of GTP by the enzyme guanylate cyclase and is degraded by phosphodiesterases. cGMP is expressed in several tissues including the brain heart and vascular smooth muscle cells. It plays significant roles in cell signaling and cellular communication.
Biological function summary

Cyclic guanosine monophosphate influences vasodilation and neurotransmission. It functions within cellular signaling complexes and often partners with proteins such as protein kinases to achieve its biological effects. cGMP facilitates the relaxation of smooth muscle tissues impacting cardiovascular health by regulating blood vessel dilation. Similarly it modulates neural signaling affecting processes like memory formation and vision.

Pathways

CGMP plays an important role in the nitric oxide signaling pathway and the atrial natriuretic peptide (ANP) pathway. Within the nitric oxide signaling pathway cGMP works in coordination with nitric oxide synthase and protein kinase G to mediate vasodilation. In the ANP pathway cGMP acts downstream of guanylate cyclase-linked receptors in maintaining cardiovascular and renal functions interconnecting with proteins like atrial natriuretic peptide to regulate blood pressure and volume.

Cyclic guanosine monophosphate is associated with hypertension and erectile dysfunction. Its role in vascular regulation links it to hypertension where decreased cGMP levels can elevate blood pressure. Additionally in erectile dysfunction cGMP imbalance disrupts proper blood flow regulation necessary for erection. Phosphodiesterase inhibitors such as sildenafil target cGMP pathways to restore normal function in these conditions highlighting the protein’s involvement in disease treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Guanosine, cyclic 3',5'-(hydrogen phosphate), monosodium salt
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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