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AB204500

Anti-CGNL1/Paracingulin antibody

3

(1 Review)

|

(2 Publications)

Rabbit Polyclonal CGNL1/Paracingulin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CGNL1 aa 650-750.

View Alternative Names

JACOP, KIAA1749, CGNL1, Cingulin-like protein 1, Junction-associated coiled-coil protein, Paracingulin

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGNL1/Paracingulin antibody (AB204500)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGNL1/Paracingulin antibody (AB204500)

Paraffin embedded human prostate tissue stained for CGNL1/Paracingulin using ab204500 at 1/2500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGNL1/Paracingulin antibody (AB204500)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGNL1/Paracingulin antibody (AB204500)

Paraffin embedded human kidney tissue stained for CGNL1/Paracingulin using ab204500 at 1/2500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGNL1/Paracingulin antibody (AB204500)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGNL1/Paracingulin antibody (AB204500)

Paraffin embedded human skeletal muscle tissue stained for CGNL1/Paracingulin using ab204500 at 1/2500 dilution in immunohistochemical analysis. Shows no positivity in myocytes cells as expected.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGNL1/Paracingulin antibody (AB204500)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CGNL1/Paracingulin antibody (AB204500)

Paraffin embedded human stomach tissue stained for CGNL1/Paracingulin using ab204500 at 1/2500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.

Western blot - Anti-CGNL1/Paracingulin antibody (AB204500)
  • WB

Supplier Data

Western blot - Anti-CGNL1/Paracingulin antibody (AB204500)

All lanes:

Western blot - Anti-CGNL1/Paracingulin antibody (ab204500) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U251 MG cell lysate

Predicted band size: 149 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CGNL1 aa 650-750. The exact immunogen used to generate this antibody is proprietary information.

Q0VF96

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CGNL1 also known as Paracingulin is a protein that plays a significant mechanical role in cellular junction assembly and maintenance. It is approximately 155 kDa in mass and functions as a cytoplasmic protein. CGNL1 localizes at tight and adherens junctions and is widely expressed in epithelial tissues. This target is involved in the stabilization of cell-cell interactions contributing to the structural integrity of cellular sheets important for epithelial function.
Biological function summary

CGNL1 contributes to the control of cell junction dynamics and is part of the cingulin family complex. CGNL1 is important in linking cytoskeletal components to junctional complexes allowing cells to maintain their polarity and adhesion. It interacts with various proteins such as ZO-1 and JAM-A to coordinate the assembly and disassembly of junctional structures. These interactions facilitate the proper functioning of tight junctions which are essential for the paracellular barrier and selective permeability.

Pathways

CGNL1 participates in the Rho GTPase signaling and Wnt signaling pathways. In the Rho GTPase pathway CGNL1 interacts with the small GTPases including RhoA and Rac1 to regulate cytoskeletal organization and junction formation. The Wnt signaling pathway connects CGNL1 to the regulation of cell proliferation and differentiation where it collaborates with proteins like beta-catenin to ensure proper signal transduction. This emphasizes its role in maintaining cellular structural integrity and communication within tissues.

CGNL1 has been linked to cancer and inflammatory bowel disease (IBD). Alterations in CGNL1 expression or function can disrupt junctional complexes leading to increased permeability and a compromised epithelial barrier which are observed in IBD. In cancer abnormal CGNL1 levels can influence cell migration and invasion contributing to tumor progression. CGNL1 interacts with proteins such as occludin and claudins connecting it to these disorders and emphasizing its impact on cellular junction integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in anchoring the apical junctional complex, especially tight junctions, to actin-based cytoskeletons.
See full target information CGNL1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:101277 PubMed37944531

2023

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxue Ren,Mingle Huang,Weixiang Weng,Yubin Xie,Yifan Wu,Shenghua Zhu,Ying Zhang,Dongming Li,Jiaming Lai,Shunli Shen,Jie Lin,Ming Kuang,Xiaoxing Li,Jun Yu,Lixia Xu

Cells 11: PubMed35681428

2022

Therapeutic Validation of GEF-H1 Using a De Novo Designed Inhibitor in Models of Retinal Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Clare Mills,Sandra A Hemkemeyer,Zerin Alimajstorovic,Chantelle Bowers,Malihe Eskandarpour,John Greenwood,Virginia Calder,A W Edith Chan,Paul J Gane,David L Selwood,Karl Matter,Maria S Balda
View all publications
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Product promise

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